SFB 807: Transport and Communication across Biological Membrans

SFB 807：跨生物膜的运输和通讯

• 批准号: 57566863
• 金额: --
• 依托单位: Goethe-Universität Frankfurt am Main
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/57566863?language=en
• 关键词: SFB; 807; Transport; Communication; across

Biological membranes are intimately associated with the evolution of life, providing a barrier to or within the cell that allows for compartmentalisation and structure formation as well as concentration of molecules. However, any living cell has to communicate via this barrier with its environment and, therefore, membranes are equipped with a multitude of membrane proteins that are required for uptake and export of molecules and for communication across this barrier (sensing of stimuli and signal transduction to intracellular targets). This pivotal role of membrane proteins is exemplified in genomes from all kingdoms of life, in which 25-30 percent of the genetic information encodes for membrane proteins. Despite their importance for the physiology of cells, a precise understanding of membrane proteins and processes are only rarely available. In particular, transport processes and information transfer are often not well resolved, even though they are of paramount interest from a biomedical standpoint of view as reflected by the fact that roughly 60 percent of the currently used medical drugs target transmembrane processes. This discrepancy between our knowledge and the importance of transmembrane processes will ensure that this research area will stay in the focus both of academic research as well as the pharmaceutical industry for years to come. The Collaborative Research Centre is designed to be a comprehensive multidisciplinary approach to study the principles and molecular mechanisms of transport of molecules and information across membranes in different cellular systems and subcellular compartments. The consortium combines a large set of biochemical, biophysical, cell and structural biological as well as computational approaches to elucidate these processes in molecular detail. This entails the determination of the chronological order of key events during a transfer cycle, their timescales and their structural bases. In particular, the presumed ability of transporters and receptors to adopt multiple conformations, which are required for completion of a full activity cycle, requires the identification of these essential conformational states and an understanding of how interchange between them occurs. We will address how these events are integrated into macromolecular complexes and signalling networks by analysing their crosstalk with other membrane proteins as well as with intra- and extracellular factors.

生物膜与生命的进化密切相关，为细胞或细胞内提供屏障，允许分隔和结构形成以及分子浓缩。然而，任何活细胞都必须通过该屏障与其环境进行通信，因此，膜配备有多种膜蛋白，这些膜蛋白是分子摄取和输出以及跨越该屏障的通信（感知刺激和向细胞内靶标进行信号转导）所必需的。膜蛋白的这种关键作用在所有生命王国的基因组中都有体现，其中 25-30% 的遗传信息编码膜蛋白。尽管它们对细胞生理学很重要，但对膜蛋白和过程的精确理解却很少。特别是，运输过程和信息传递通常没有得到很好的解决，尽管从生物医学的角度来看它们是最重要的，目前使用的医疗药物中大约 60% 都针对跨膜过程，这一事实就反映了这一点。我们的知识与跨膜过程的重要性之间的这种差异将确保该研究领域在未来几年将继续成为学术研究和制药行业的焦点。该合作研究中心旨在成为一个综合性的多学科方法，研究不同细胞系统和亚细胞区室中分子和信息跨膜运输的原理和分子机制。该联盟结合了大量的生物化学、生物物理、细胞和结构生物学以及计算方法，以分子细节阐明这些过程。这需要确定传输周期中关键事件的时间顺序、它们的时间尺度和它们的结构基础。特别是，假定转运蛋白和受体具有完成完整活动周期所需的多种构象的能力，需要识别这些基本的构象状态并了解它们之间的交换是如何发生的。我们将通过分析这些事件与其他膜蛋白以及细胞内和细胞外因子的串扰来解决这些事件如何整合到大分子复合物和信号网络中。

项目成果

The c-ring stoichiometry of ATP synthase is adapted to cell physiological requirements of alkaliphilic Bacillus pseudofirmus OF4

• DOI: 10.1073/pnas.1303333110
• 发表时间: 2013-05-07
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Preiss, Laura;Klyszejko, Adriana L.;Meier, Thomas
• 通讯作者: Meier, Thomas

Photocycle-dependent conformational changes in the proteorhodopsin cross-protomer Asp–His–Trp triad revealed by DNP-enhanced MAS-NMR

• DOI: 10.1073/pnas.1817665116
• 发表时间: 2019-04
• 期刊: Proceedings of the National Academy of Sciences
• 作者: Jakob Maciejko;J. Kaur;Johanna Becker‐Baldus;C. Glaubitz

Alternating-access mechanism in conformationally asymmetric trimers of the betaine transporter BetP

• DOI: 10.1038/nature11403
• 发表时间: 2012-10-04
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Perez, Camilo;Koshy, Caroline;Ziegler, Christine
• 通讯作者: Ziegler, Christine

Promiscuous archaeal ATP synthase concurrently coupled to Na+ and H+ translocation

• DOI: 10.1073/pnas.1115796109
• 发表时间: 2012-01-17
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Schlegel, Katharina;Leone, Vanessa;Mueller, Volker
• 通讯作者: Mueller, Volker

Ultrafast infrared spectroscopy on channelrhodopsin-2 reveals efficient energy transfer from the retinal chromophore to the protein.

• DOI: 10.1021/ja400554y
• 发表时间: 2013-05
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Mirka Neumann-Verhoefen;Karsten Neumann;C. Bamann;I. Radu;J. Heberle;E. Bamberg;J. Wachtveitl