FOR 1061: Dynamic Storage Functions of Plant Vacuoles During Cold and Osmotic Stress

FOR 1061：植物液泡在冷和渗透胁迫下的动态储存功能

• 批准号: 61498647
• 金额: --
• 依托单位: Fachgebiet Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/61498647?language=en
• 关键词: 1061; Dynamic; Storage; Functions; Plant

The vacuole represents the largest plant cell organell with several storage functions. In general, plants cannot escape from their location and, therefore, have to adapt to permanently changing environmental conditions. During the onset of cold or osmotic stress, many solutes like, e.g., sugars, sugar alcohols, organic acids, amino acids, potassium and other ions accumulate in the vacuole. This process contributes to the development of cellular stress tolerance. Especially the cell-internal compartimentation of individual molecules is changed and the rate of biosyntheses of suitable solutes must be rapidly adapted. The underlying transport processes and the biosynthesis, respectively degradation pathways will be in detail analysed. In mutant plants lacking one or several proteins, or in which the activity of one or several proteins are overexpressed, changes in the stress tolerance will be tested. In the special focus are vacuolar sugar- and sugar-alcohol transporter, malate carrier as well as channel proteins. In addition, stress induced changes of the vacuolar energisation by the tonoplast V-ATPase or V-pyrophosphatase will be enlightened. The methods used enclose molecular techniques as well as biochemical and biophysical technologies, recombinant proteins, mass-spectrometric proteome analysis and microscopic techniques. We expect the outcome of our research contributes to the understanding of molecular stress physiology.

液泡是植物细胞中最大的细胞器，具有多种贮藏功能。一般来说，植物无法逃离它们的位置，因此必须适应不断变化的环境条件。在寒冷或渗透胁迫的开始期间，许多溶质，例如，糖、糖醇、有机酸、氨基酸、钾和其它离子在液泡中积累。这一过程有助于细胞应激耐受性的发展。特别是细胞内单个分子的比较发生了变化，必须迅速调整合适溶质的生物合成速率。将详细分析潜在的运输过程和生物合成，分别降解途径。在缺乏一种或几种蛋白质或其中一种或几种蛋白质的活性过表达的突变体植物中，将测试胁迫耐受性的变化。特别关注的是液泡糖和糖醇转运蛋白，苹果酸载体以及通道蛋白。此外，对液泡膜V-ATPase或V-Pyrophosphatase在胁迫诱导的液泡供能过程中的变化也有一定的启示。所使用的方法包括分子技术以及生物化学和生物物理技术、重组蛋白、质谱蛋白质组分析和显微镜技术。我们希望我们的研究结果有助于分子应激生理学的理解。