Interaction of bacterial actin-like proteins with the cell membrane

细菌肌动蛋白样蛋白与细胞膜的相互作用

• 批准号: 61723844
• 负责人: Professor Dr. Peter Graumann
• 金额: --
• 依托单位: École Supérieure de Biotechnologie (ESBS)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/61723844?language=en
• 关键词: Interaction; bacterial; actin; like; proteins

Many prokaryotic cells contain a homolog of actin, called MreB, which shares many conserved properties with actin, and is essential for cell shape maintenance and viability in many bacteria. We have found that it localizes as dynamic helical filaments underneath the cell membrane, together with its two paralogs, Mbl and MreBH. MreB and Mbl interact with two membrane proteins, MreC and RodA, involved in cell shape maintenance, as well as with cytosolic proteins, many of which have an enzymatic function. MreB also interacts with translation factor EF-Tu along the helical tracks, and EF-Tu is also important for cell shape maintenance, showing that EF-Tu serves a dual role in a bacterial cell. EF-Tu shows a similar spectrum of interactions as MreB, suggesting that both proteins set up an interaction network underneath the bacterial cell membrane. Within the FOR, we will search for further evidence for this network and will investigate its role in bacterial physiology. We will also study in detail how MreB and Mbl direct the positioning of membrane proteins, which is the case for RodA, whose function in cell morphology we have begun to study and want to continue to elucidate, amongst others by determining its 3D structure. We will also study the function of membrane protein RodZ, which affects cell shape in an unknown manner in many bacteria.

许多原核细胞含有肌动蛋白的同源物，称为MreB，它与肌动蛋白具有许多保守特性，对许多细菌的细胞形状维持和活力至关重要。我们发现它与它的两个类似物Mbl和MreBH一起定位于细胞膜下的动态螺旋状细丝。MreB和Mbl与参与细胞形状维持的两种膜蛋白MreC和RodA以及细胞质蛋白相互作用，其中许多具有酶促功能。MreB还沿着螺旋轨道与翻译因子EF-Tu相互作用，EF-Tu对细胞形状维持也很重要，这表明EF-Tu在细菌细胞中起双重作用。EF-Tu显示出与MreB相似的相互作用谱，这表明这两种蛋白质在细菌细胞膜下建立了一个相互作用网络。在FOR中，我们将寻找该网络的进一步证据，并将研究其在细菌生理学中的作用。我们还将详细研究MreB和Mbl如何指导膜蛋白的定位，这是RodA的情况，我们已经开始研究其在细胞形态中的功能，并希望通过确定其3D结构等继续阐明。我们还将研究膜蛋白RodZ的功能，它以一种未知的方式影响许多细菌的细胞形状。

项目成果

Superresolution imaging of dynamic MreB filaments in B. subtilis--a multiple-motor-driven transport?

枯草芽孢杆菌中动态 MreB 丝的超分辨率成像——多电机驱动的运输？

• DOI: 10.1016/j.bpj.2013.07.038
• 发表时间: 2013
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: v. Olshausen P;H. J. Defeu Soufo;K. Wicker;R. Heintzmann;P. L. Graumann;A. Rohrbach
• 通讯作者: A. Rohrbach

Translation elongation factor EF-Tu modulates filament formation of actin-like MreB protein in vitro.

翻译延伸因子 EF-Tu 体外调节肌动蛋白样 MreB 蛋白丝的形成

• DOI: 10.1016/j.jmb.2015.01.025
• 发表时间: 2015
• 期刊: Journal of molecular biology
• 影响因子: 5.6
• 作者: Defeu Soufo HJ;Reimold C;Breddermann H;Mannherz HG;Graumann PL
• 通讯作者: Graumann PL