Effects of prenatal perfluorinated chemicals exposure and genetic vulnerability on glucocorticoids hormone profiles, growth and risk of infectious diseases in next generation

产前全氟化学品暴露和遗传脆弱性对下一代糖皮质激素谱、生长和传染病风险的影响

• 批准号: 26740028
• 负责人: GOUDARZI Houman
• 金额: $ 2.66万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2014
• 资助国家: 日本
• 起止时间: 2014-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26740028/
• 关键词: perfluorinated chemicals; Glucocorticoid hormones

In this study, we are trying to clarify the effects of prenatal exposure to perfluorinated chemicals on Glucocorticoid hormones in next generation.Among whole participants in Toho cohort (n=514), participants with available perinatal PFOS/PFOA levels in serum samples obtained in Sapporo Toho cohort were included in this study (n=295) after exclusion of twins and preterm births. Maternal PFOS and PFOA have been measured after the second trimester of pregnancy in serum samples by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Baseline questionnaires, birth records and follow-up questionnaires were collected during pregnancy until 7 years old including information on parental demographic characteristics, dietary habits, socioeconomic status, birth size, children’s growth and infectious diseases (data cleaning has been performed). Genetic polymorphisms were assessed in last summer to determine of maternal (blood samples taken at delivery) and infant (cord blood samples) single nucleotide polymorphisms (SNPs) of glucocorticoid receptors. Genetic analysis were performed by EP1 system (Fluidigm). Cortisol and cortisone levels in cord blood samples of 130 randomly selected subjects have been measured by LC-MS-MS, and I started to do data analysis.

在这项研究中，我们试图澄清产前接触全氟化学品对下一代糖皮质激素的影响。在Toho队列的全部参与者（n=514）中，排除双胞胎和早产后，在札幌Toho队列中获得围产期PFOS/PFOA血清样本的参与者（n=295）被纳入本研究。采用液相色谱-串联质谱法（LC-MS-MS）测定了妊娠中期后产妇血清样品中的全氟辛烷磺酸和全氟辛烷磺酸。在怀孕至7岁期间收集了基线问卷、出生记录和后续问卷，包括父母的人口特征、饮食习惯、社会经济地位、出生规模、儿童成长和传染病等信息（已进行数据清理）。去年夏天对遗传多态性进行了评估，以确定母体（分娩时采集的血液样本）和婴儿（脐带血样本）糖皮质激素受体的单核苷酸多态性（snp）。采用EP1系统（Fluidigm）进行遗传分析。用LC-MS-MS测量了随机选取的130名受试者脐带血样本中的皮质醇和可的松水平，并开始进行数据分析。