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Allelopathic effects of submerged macrophytes on phytoplankton: In situ evidence, bacterial influence and new modes of action

沉水植物对浮游植物的化感作用：原位证据、细菌影响和新的作用模式

基本信息

批准号：
63690330
负责人：
Professorin Dr. Sabine Hilt
金额：
--
依托单位：
Abteilung 2: Ökosystemforschung
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2008
资助国家：
德国
起止时间：
2007-12-31 至 2012-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/63690330?language=en
关键词：
Allelopathic effects submerged macrophytes phytoplankton

项目摘要

Inhibition of phytoplankton by allelochemicals released by submerged macrophytes is thought to be one of the mechanisms that contribute to the stabilisation of clear-water states in shallow lakes. Despite numerous studies showing the inhibiting effect of plant extracts on phytoplankton, the relevance of this process at ecosystem level is still debated. In situ evidence is difficult to achieve and a number of important details of allelopathic interactions are unknown. The proposed project aims to provide in situ evidence of alielopathic effects of the macrophyte species Myriophyllum verticillatum, which is known to contain allelopathically active substances, on phytoplankton using dialysis bag experiments in a small lake. Field and laboratory experiments will be carried out to test whether specific algae-associated bacteria metabolise allelochemicals and thus contribute to the observed differential sensitivity of algal species. New modes of action of polyphenols, a common group of allelochemicals, will be investigated in different algae species using fluorescent dye staining flow cytometry. Purified compounds from M. verticillatum will be chemically identified and their effects on phytoplankton tested using PAM fluorometry and flow cytometry. We expect a significant contribution to the understanding of alielopathic interactions in the aquatic environment.

沉水植物释放的化感物质对浮游植物的抑制作用被认为是浅水湖泊保持清澈状态的机制之一。尽管许多研究表明植物提取物对浮游植物的抑制作用，但这一过程在生态系统水平上的相关性仍有争议。原位证据是很难实现的，一些重要的细节化感作用是未知的。拟议的项目旨在利用一个小湖的透析袋实验，提供已知含有化感活性物质的大型植物物种狐尾藻对浮游植物的化感作用的现场证据。将进行实地和实验室实验，以测试特定的藻类相关细菌是否代谢化感物质，从而有助于观察到的藻类物种的差异敏感性。利用荧光染料染色流式细胞术研究多酚类物质在不同藻类中的新作用方式。从M.将用化学方法鉴定轮枝藻，并使用PAM荧光测定法和流式细胞术测试它们对浮游植物的影响。我们期待着一个重大的贡献，了解alielopathic在水生环境中的相互作用。