Physiological function of nucleoside / nucleobase transport and catabolism

核苷/核碱基转运和分解代谢的生理功能

• 批准号: 66598109
• 负责人: Dr. Torsten Möhlmann
• 金额: --
• 依托单位: Fachgebiet Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/66598109?language=en
• 关键词: Physiological; function; nucleoside; nucleobase; transport

In humans nucleoside transporters execute diverse functions, inter alia through interaction with purinergic receptors. In addition, nucleoside analogs are used to treat cancer. In plants, Equilibrative Nucleoside Transporter 1 (ENT1) function was found to be critical for pollen development, seed germination and maintaining the overall nucleotide balance. We propose to unravel the physiological reason leading to the observed phenotype in more detail. A focus will be on the question to what extend ENT1 function is involved in extracellular ATP signalling. Unexpectedly, ENT1 activity was found to be connected to vacuolar RNA metabolism. Therefore, this aspect shall be highlighted by analyzing vacuolar RNA (contents, nature of RNA, import mechanism) in Arabidopsis. In addition, Barley will be introduced as model system in our lab with respect to vacuolar RNA metabolism. Further aspects of the proposed work are related to nucleoside catabolism. In the last funding period novel proteins in this pathway could be identified. Among these, the extracellular nucleoside hydrolase NSH3 and the plastidic uracil importer Pluto have so far only been analyzed preliminary. A more detailed analysis of the impact of these proteins will be performed to complete our understanding of extracellular and intracellular nucleoside catabolism.

在人类中，核苷转运蛋白执行不同的功能，阿利亚是通过与嘌呤能受体的相互作用。此外，核苷类似物用于治疗癌症。在植物中，平衡核苷转运蛋白1（ENT1）的功能被发现是至关重要的花粉发育，种子萌发和维持整体核苷酸平衡。我们建议解开的生理原因，导致观察到的表型更详细。一个重点将是什么扩展ENT1功能参与细胞外ATP信号传导的问题。出乎意料的是，ENT1活性被发现与液泡RNA代谢有关。因此，通过对拟南芥液泡RNA的含量、RNA的性质、导入机制等方面的分析，可以突出这一点。此外，大麦将作为本实验室研究液泡RNA代谢的模型系统。所提出的工作的进一步方面与核苷催化剂有关。在上一个资助期内，可以鉴定出该途径中的新蛋白质。其中，细胞外核苷水解酶NSH 3和质体尿嘧啶输入者Pluto迄今为止仅进行了初步分析。对这些蛋白质的影响进行更详细的分析，以完成我们对细胞外和细胞内核苷催化剂的理解。