Aerosolized Epigenetic Therapy for Metastatic Lung Cancer

雾化表观遗传疗法治疗转移性肺癌

• 批准号: 10760630
• 负责人: Steven A Belinsky
• 金额: $ 86.11万
• 依托单位: NOB HILL THERAPEUTICS, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-19 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10760630
• 关键词: Acute leukemia; Aerosols; Air; Air Movements; Azacitidine; Brain; Breathing; Cancer Etiology; Cancer Model; Cancer Patient; Catabolism; Cells; Cessation of life; Characteristics; Chemicals; Chronic Obstructive Pulmonary Disease; Clinic; Clinical Research; Combination Drug Therapy; Cytidine Deaminase; Cytosine; Data; Deoxycytidine; Devices; Diagnosis; Disease; Documentation; Dose; Drug Kinetics; Dryness; Dysmyelopoietic Syndromes; Emulsions; Enzyme Inhibition; Epigenetic Process; Europe; Evaluation; FDA approved; Forced expiratory volume function; Formulation; Gene Expression; Gene Silencing; Genes; Genetic; Genetic Transcription; Half-Life; Hour; Human; Hypermethylation; Immune; Immunotherapy; Inhalation; Inhalation Toxicology; Inhalators; Injections; Investigational Drugs; Label; Legal patent; Liver; Lung; Lung Neoplasms; Malignant Neoplasms; Malignant neoplasm of lung; Methylation; Modeling; Nebulizer; Particle Size; Patients; Performance; Pharmaceutical Preparations; Phase; Phase I Clinical Trials; Powder dose form; Practice Guidelines; Progression-Free Survivals; Promoter Regions; Rattus; Regulatory Pathway; Relapse; Reporting; Research; Resistance; Rodent; Rodent Model; Safety; Signal Pathway; Small Business Innovation Research Grant; Solid Neoplasm; Stream; Technology; Testing; Therapeutic; Tissues; Toxicology; Tumor Burden; Work; absorption; aerosolized; aqueous; chemotherapy; epigenetic therapy; first-in-human; good laboratory practice; immune activation; improved; in vivo; innovation; manufacture; novel; nucleoside analog; phase III trial; pre-clinical; pressure; programs; promoter; pulmonary function; quality assurance; research clinical testing; response; safety study; scale up; standard of care; success; transcriptome; transcriptome sequencing; tumor; tumor heterogeneity

Project Summary Lung cancer (LC) with over 2 million cases/year worldwide also remains the leading cause of cancer- related death in the US and Europe. Treating LC remains challenging due to ~60% of patients presenting with Stage IV disease, the heterogeneity of tumors with respect to genetic and epigenetic mechanisms that alter gene expression, and treatment-related resistance. Combining chemotherapy with immunotherapy as the first treatment option for advanced LC has significantly improved response rates and survival; however, median duration for progression free survival (PFS) was still 8.8 versus 4.9 months for Stage IV LC patients receiving chemo/immune versus chemotherapy with 69% and 49% alive at one year, respectively. Second line drugs after relapse provide minimal benefit. Epigenetic deregulation involves cytosine methylation in the promoter region of hundreds of genes to impede transcription leading to loss of expression. Thus, a therapy that could reverse methylation and awaken these genes could produce durable and sustained tumor regression. The cytosine nucleoside analogs 5-azacytidine (5AZA) and 5-aza-2’-deoxycytidine (DAC) inhibit the enzyme responsible for cytosine methylation resulting in re-expression of genes silenced through cytosine promoter hypermethylation. These FDA approved drugs are serving as potent therapy for blood-borne cancers, myelodysplasia and acute leukemia. Epigenetic therapy for solid tumors including LC has been impacted by the poor stability in an aqueous solution of 5AZA and DAC and catabolism by cytidine deaminase in liver and GI. Lovelace Biomedical working with Bend Research/Lonza have overcome these barriers by developing a stable dry powder (DP) formulation of 5AZA (5AZA-DP). The novel dry powder nebulizer (DryNeb) developed by Nob Hill Therapeutics generates optimal aerosol particle sizes for local lung delivery-activity and optimal systemic absorption characteristics while facilitating patients’ “easy” tidal breathing inhalation with no forced pulmonary function maneuvers. In studies in which 5AZA-DP was delivered to tidally breathing rats, superior pharmacokinetics were achieved in the liver, and most impressively to the brain with a half-life of 4 hours when compared with injected aqueous solutions of 5AZA. Using a rat lung tumor model developed from instilling cells obtained from human lung tumors, tidally inhaled 5AZA-DP reduced tumor burden by 70–95% for the 4 different non-small cell lung tumors evaluated, far exceeding the 32% reduction seen with systemic injection of 5AZA. A proof-of-concept clinical study showed stable localized lung cancer in 3 of 8 Phase I patients treated with nebulized aqueous 5AZA. This Phase II SBIR through three specific aims builds on these exciting data by focusing on performing formulation scale up and stability optimization of 5AZA-DP, aerosol performance in the DryNeb, and GLP toxicology studies. Completion of these aims will support filing an investigational drug application (IND) to the FDA to allow evaluation of 5AZA-DP using DryNeb in a Phase I dose escalation safety study followed by a Phase IB study in LC patients who have relapsed on standard-of-care therapy.

项目总结