Molecular phylogeny, biogeography and evolution of tropical African Thelephorales

热带非洲泰勒目的分子系统发育、生物地理学和进化

• 批准号: 71370276
• 负责人: Professor Dr. Reinhard Agerer
• 金额: --
• 依托单位: Bereich Systematik, Biodiversität & Evolution der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/71370276?language=en
• 关键词: Molecular; phylogeny; biogeography; evolution; tropical

Thelephorales (Agaricomycetes, Basidiomycota) constitute a monophyletic ectomycorrhizal fungal group that encompasses presently a total of 177 species and two families. Though they have a worldwide distribution, Thelephorales from the tropics are scantily documented. Using a combination of anatomical and molecular studies of fruit bodies and ectomycorrhizae (EcM), the present project aims at addressing the phylogenetic positions of tropical African species and their relevance for the evolutionary interpretation of Thelephorales in general. Specimens (fruit bodies and EcM) will be collected within tropical African woodlands and forests dominated by ectomycorrhizal trees following two major megatransects. We select Megatransects in respect of changes in vegetation and floristic composition in order to better assess the influence of both parameters on diversity and distribution of Thelephorales. Collected fruit bodies and EcM will be conserved for further anatomical and molecular investigations. In addition, dried material of fruit bodies will be borrowed from international herbaria. For intra-generic phylogenetic assessment, we will amplify the Internal Transcribed Spacers of rDNA, and for generic-level phylogeny, partial nuclear rDNA Large SubUnit (LSU). Phylogenetic analysis will be undertaken on sequences we will generate against those obtained from temperate and boreal thelephoroid species (available in public Genbanks). Anatomical description will be given for representative fruit bodies and EcM; following internationally recognised methods. We aim at a monograph of common tropical African Thelephorales coupled with an on-line interactive identification key, molecular and anatomical characterisation of EcM, description of new species, and resolving their phylogenetic positions.

Thelephorales（琼脂菌，基菌）构成了一个单系的外生科体真菌群，该集团涵盖目前总共有177种和两个家庭。尽管它们具有全球分布，但来自热带地区的Thelephorales却有很少的记录。本项目结合了水果体的解剖学和分子研究（ECM），目前的项目旨在解决热带非洲物种的系统发育位置及其与一般而言的塞勒普拉群岛进化解释的相关性。标本（水果体和ECM）将在热带非洲林地内收集，以及两种主要的大型坦率的森林，由外生菌树占主导地位。我们选择大趋化的植被和植物组成的变化，以更好地评估这两个参数对thephorales多样性和分布的影响。收集的水果体和ECM将被保存，以进行进一步的解剖和分子研究。此外，将从国际草药中借用水果体的干材料。为了进行基因内的系统发育评估，我们将放大rDNA的内部转录间隔物，以及用于通用的系统发育，部分核RDNA大型亚基（LSU）。系统发育分析将对我们将针对从温带和北方thephoroid物种获得的序列进行（可在公共GenBanks中使用）。将为代表性的水果体和ECM提供解剖学描述；遵循国际公认的方法。我们旨在将普通的热带非洲thephorales的专着以及在线互动识别键，ECM的分子和解剖学表征，新物种的描述以及解决其系统发育位置的典范。