Molecular phylogeny, biogeography and evolution of tropical African Thelephorales

热带非洲泰勒目的分子系统发育、生物地理学和进化

• 批准号: 71370276
• 负责人: Professor Dr. Reinhard Agerer
• 金额: --
• 依托单位: Bereich Systematik, Biodiversität & Evolution der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/71370276?language=en
• 关键词: Molecular; phylogeny; biogeography; evolution; tropical

Thelephorales (Agaricomycetes, Basidiomycota) constitute a monophyletic ectomycorrhizal fungal group that encompasses presently a total of 177 species and two families. Though they have a worldwide distribution, Thelephorales from the tropics are scantily documented. Using a combination of anatomical and molecular studies of fruit bodies and ectomycorrhizae (EcM), the present project aims at addressing the phylogenetic positions of tropical African species and their relevance for the evolutionary interpretation of Thelephorales in general. Specimens (fruit bodies and EcM) will be collected within tropical African woodlands and forests dominated by ectomycorrhizal trees following two major megatransects. We select Megatransects in respect of changes in vegetation and floristic composition in order to better assess the influence of both parameters on diversity and distribution of Thelephorales. Collected fruit bodies and EcM will be conserved for further anatomical and molecular investigations. In addition, dried material of fruit bodies will be borrowed from international herbaria. For intra-generic phylogenetic assessment, we will amplify the Internal Transcribed Spacers of rDNA, and for generic-level phylogeny, partial nuclear rDNA Large SubUnit (LSU). Phylogenetic analysis will be undertaken on sequences we will generate against those obtained from temperate and boreal thelephoroid species (available in public Genbanks). Anatomical description will be given for representative fruit bodies and EcM; following internationally recognised methods. We aim at a monograph of common tropical African Thelephorales coupled with an on-line interactive identification key, molecular and anatomical characterisation of EcM, description of new species, and resolving their phylogenetic positions.

革菌目（Thelephorales）是伞菌纲（Agaricomycetes）、担子菌门（Basidiomycota）的一个单系外生菌根真菌类群，目前共有2科177种。虽然它们有一个世界性的分布，Thelephorales从热带地区是很少的文件。利用子实体和外生菌（EcM）的解剖和分子研究相结合，本项目的目的是解决热带非洲物种的系统发育位置和Thelephorales的进化解释一般的相关性。将在热带非洲林地和两个主要大断面后以外生菌根树为主的森林内采集标本（子实体和外生菌根）。为了更好地评估这两个参数对Thelephorales多样性和分布的影响，我们选择了植被和区系组成的变化方面的大断面。收集的子实体和外鞘细胞将被保存用于进一步的解剖和分子研究。此外，子实体的干燥材料将从国际标本馆借用。对于属内系统发育评估，我们将扩增rDNA的内转录间隔区，对于属水平系统发育，扩增部分核rDNA大亚基（LSU）。系统发育分析将进行序列，我们将产生对那些从温带和北方thelephoroid物种（可在公共基因库）。解剖描述将给出代表性的子实体和外鞘细胞;以下国际公认的方法。我们的目标是在一个专着的共同热带非洲Thelephorales加上一个在线的互动识别键，分子和解剖特征的外膜，新物种的描述，并解决其系统发育的位置。