Bone resorptive activity of periodontal tissues associatecl with experimental tooth movement

牙周组织骨吸收活性与实验性牙齿移动的关系

• 批准号: 59570874
• 负责人: YAMASAKI Kenichi
• 金额: $ 0.99万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59570874/
• 关键词: orthodontic tooth movement; bone resorptive activity; periodontal tissue organ culture; chemical mediator; Prostaglandin E; プロスタサイクリン; トロンボキサンA_2; 【^(45)Ca】; 凍結割断; ケミカルメディエーター; Waldo法

The aim of the present study are to transfer the periodontal tissues received experimental tooth movement into organ culture(in vitro) system,and to measure the bone resporptive activity of them. Using this system will clear up mechanizms of many kind of drugs and hormones on the rate of orthodontic tooth movement. 1. Establishment of experimental tooth movement; Rat incisors were fitted lateral expanding helical torsion loops(30g) During 1 day to 7 days. This experimepnt induced the appearance of osteoclasts and bone resorption of buccal site of alveolar bone.2.Establishment of bone culture system for measurement of bone resorptive activity ;Neo-natal mice(1-day-old) were labelled^<45>Cacl_2. 4 days later,calvariae were dissected out and transferred to BGJB medium supplemented with 5% horse serum and antibiotics. Addition of PTH,PGE_2,PGl_2 analog(Op41483) and TXA_2 analog(STA_2) to this culture medium induced bone resorption (the rate of ^<45>Ca release from calvariae). So,this system will be able to measure the bone resorptive activity in vitro.3. Examination of organ culture of periodontal tissues received experimental tooth movement; The extracted periodontal tissues showed life-activity within 24 hours in the above organ culture system.4.The effect of periodontal tissues received experimental tooth movement on bone resorption in vitro; In spite of difficulty of preparation of experiment in vitro system,the difference of bone resorptive activity between control and treated periodontal tissues can be detectable.

本研究的目的是将实验性牙齿运动转移到器官培养物（体外）系统中，并测量其骨骼的骨骼分裂活性。使用该系统将在正畸牙齿运动速率上清除多种药物和激素的机械化。 1。建立实验性牙齿运动；在1天到7天内安装了大鼠门牙，横向扩展螺旋扭转环（30G）。该实验诱导牙槽骨骨骼的破骨细胞和骨吸收的出现。2。骨培养系统的建立以测量骨骨化活性；新生儿小鼠（1天）被标记为^<45> cacl_2。 4天后，剖析了钙钙，并将其转移到补充了5％马血清和抗生素的BGJB培养基中。添加PTH，PGE_2，PGL_2模拟（OP41483）和TXA_2模拟（STA_2）在此培养基中诱导骨吸收（从Calvariae释放 ^<45> Ca的速率）。因此，该系统将能够在体外测量骨骼吸收活性3。检查牙周组织的器官培养，接受了实验性牙齿运动；提取的牙周组织在上述器官培养系统中显示出24小时内的生命活性。4。牙周组织的作用接受了实验性牙齿运动对体外骨吸收的影响。尽管难以在体外系统中制备实验，但可以检测到对照和处理过的牙周组织之间骨吸收活性的差异。