Sec14様タンパク質による低リン環境での植物成長の改善の分子メカニズムの解明

在第一年（2022年4月至2023年3月），我能够在我计划的研究中取得进展，并取得了良好的结果，正如我所期望的那样。本课题的研究进展如下：（1）对6份多态性较强的材料的LPU 1启动子进行了瞬时活性检测，结果表明这些材料的LPU 1启动子活性明显高于Col.0。结果表明，启动子中发现的一些核苷酸多态性负责其更有效的活性，导致相应的加入更高的LPU 1表达水平和Pi吸收活性。(2)I研究了对照和低磷条件下生长的WT、oslpu 1-KO和OsLPU 1-OX幼苗叶片和纯化叶绿体中4种磷脂（PI、PA、PG和PC）和3种糖脂（MGDG、DGDG和SQDG）的含量。我发现在对照条件下，WT、oslpu 1-KO和OsLPU 1-OX幼苗的脂质含量几乎相同。在缺磷条件下，磷缺乏引起的磷脂和糖脂含量的变化减轻oslpu 1-KO幼苗。还进行了主成分分析（PCA）。在对照和低Pi条件下观察到的两个谱在来自WT和OsLPU 1-OX植物的样品中明显分离，但在来自oslpu 1-KO植物的样品中没有分离。

In the first year (April 2022 - March 2023), I was able to make progress on my planned research and obtained good results, as I expected. My project progress during this year is as follows:(1)I performed transient assay using the LPU1 promoters of six accessions which contain many polymorphisms, and revealed that LPU1 promoters of these accessions had remarkably stronger activity than Col.0. The result suggested that some of the nucleotide polymorphisms found in promoters are responsible for their more potent activity, leading to higher LPU1 expression levels and Pi uptake activity in the corresponding accessions.(2)I investigated the contents of four phospholipids (PI, PA, PG, and PC) and three glycolipids (MGDG, DGDG, and SQDG) in the leaves and purified chloroplasts of WT, oslpu1-KO and OsLPU1-OX seedlings grown under control and low Pi condition. I found under control condition, the lipid content of WT, oslpu1-KO and OsLPU1-OX seedlings was almost the same. Under Pi-deficient conditions, Pi deficiency-induced changes in phospholipid and glycolipid contents were alleviated in oslpu1-KO seedlings. Principal component analysis (PCA) was also performed. Two profiles observed under control and low Pi conditions were evidently separated in samples from WT and OsLPU1-OX plants but not in samples from oslpu1-KO plants.