Sec14様タンパク質による低リン環境での植物成長の改善の分子メカニズムの解明

在第一年（2022年4月- 2023年3月），我的计划研究取得了进展，并取得了预期的好成绩。我这一年的项目进展情况如下：(1)利用6个包含较多多态性的种质的LPU1启动子进行瞬态分析，发现这些种质的LPU1启动子的活性明显强于Col.0。结果表明，在启动子中发现的一些核苷酸多态性导致了它们更有效的活性，从而导致相应材料中更高的LPU1表达水平和Pi摄取活性。(2)研究了在控制和低PI条件下生长的WT、oslpu1-KO和OsLPU1-OX幼苗叶片和纯化叶绿体中四种磷脂（PI、PA、PG和PC）和三种糖脂（MGDG、DGDG和SQDG）的含量。我发现在对照条件下，WT、oslpu1-KO和OsLPU1-OX幼苗的脂质含量几乎相同。在缺磷条件下，缺磷引起的oslpu1-KO幼苗磷脂和糖脂含量变化得到缓解。并进行主成分分析（PCA）。在对照和低Pi条件下观察到的两个基因型在WT和OsLPU1-OX植株中明显分离，而在oslpu1-KO植株中没有分离。

In the first year (April 2022 - March 2023), I was able to make progress on my planned research and obtained good results, as I expected. My project progress during this year is as follows:(1)I performed transient assay using the LPU1 promoters of six accessions which contain many polymorphisms, and revealed that LPU1 promoters of these accessions had remarkably stronger activity than Col.0. The result suggested that some of the nucleotide polymorphisms found in promoters are responsible for their more potent activity, leading to higher LPU1 expression levels and Pi uptake activity in the corresponding accessions.(2)I investigated the contents of four phospholipids (PI, PA, PG, and PC) and three glycolipids (MGDG, DGDG, and SQDG) in the leaves and purified chloroplasts of WT, oslpu1-KO and OsLPU1-OX seedlings grown under control and low Pi condition. I found under control condition, the lipid content of WT, oslpu1-KO and OsLPU1-OX seedlings was almost the same. Under Pi-deficient conditions, Pi deficiency-induced changes in phospholipid and glycolipid contents were alleviated in oslpu1-KO seedlings. Principal component analysis (PCA) was also performed. Two profiles observed under control and low Pi conditions were evidently separated in samples from WT and OsLPU1-OX plants but not in samples from oslpu1-KO plants.