INTRACELLULAR SIGNAL TRANSDUCTION IN DICTYOSTELIUM ANALYZED BY INSERTION MUTAGENESIS

渗透波动是所有生物的主要压力。我们一直在研究细胞对渗透胁迫的反应，以变形虫盘基骨为模型系统。我们通过重组酶介导的质粒插入诱导随机诱变后，筛选了在渗透胁迫下可能具有缩短表型的突变菌落。我们建立了一种同时监测许多突变体渗透反应的方法。通过这种方法，我们发现在高渗和低渗条件下，大约一半的筛选菌落在生长发育中具有各种缩短表型。我们计划分析这些突变体中被破坏的基因。

Osmotic fluctuation is the major stress in all living organisms. We have been studying cellular responses to osmotic stress using Dictyostelium amoeba as a model system. We screened mutant colonies which potentially have abbreviated phenotypes in response to osmotic stress after the induction of the random mutagenesis by recombinant enzyme-mediated plasmid insertion. We established a method to monitor osmotic response in many mutants at once. By this method we found about half of the screened colonies had variety of abbreviated phenotypes in growth and development under hypertonic and hypotonic conditions. We plan to analyze destructed genes in these mutants.