Gene analysis of pectral finless mutant (pl) of Japanese Medaka(Oryzias latipes)

(A)回交HNI/Pl(雌性)×Lp/Pl(雄性)：从3组回交组合中获得F1后代(597个)。其中野生型278株(46.6%)，pl=317株(53.1%)，lpl=2株(0.3%)。我们又检查了500个后代，发现LPL的出现频率大致相同。(2)LPL-L(雄性)×LPL-2(雌性)：PL=90(53%)，LPL=43(25%)，野生型=38(22%)。我们观察了每个胚胎的早期发育阶段，发现大约一半(47)的PL在早期胚胎形成鳍芽，然后消失。(C)LPL-1×pl/pl：pl与lpl的比例不相等，但大多数后代是pl，约10%是lpl。根据这些结果，我们推测LPL突变体的表达可能不是由单基因调控的，而是由多个基因参与的。

(a)backcross HNI/pl (female) x lp/pl (male): F1 offsprings (597) were obtained from 3 sets of backcross pair. Among them wildtype were 278 (46.6 %), pl=317 (53.1 %) and lpl=2 (0.3 %). We checked 500 offsprings more and found that frequency of appearance of lpl was about the same. The frequency of lpl appearance seems to be high.(b) lpl-l (male) x lpl-2 (female): pl=90 (53 %), lpl=43 (25 %), wild type=38 (22 %), We observed early developmental stage of each embryos and found that approximately half (47) of the pl formed fin bud at early embryo stage and they disappeared after then.(c) lpl-1 x pl/pl: The ratio of pl to lpl was not equal but most of the offsprings were pl and approximately 10 % were lpl. From these results we speculated that expression of lpl mutant might not be regulated by single gene but more than two genes take part in this event.