Development of the novel evaluation used for measurement of distress and discomfort in the vascular pain model of guinea pigs.

开发用于测量豚鼠血管疼痛模型中的痛苦和不适的新型评估。

• 批准号: 09680834
• 负责人: ANDO Ryuichiro
• 金额: $ 0.38万
• 依托单位: Tohoku College of Pharmacy
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680834/
• 关键词: Vascular pain model; Tachykinins; Excitatory animo acids; Electrocardiogram; 1; f fluctuation; Distress; Discomfort; Vocalization; モルモット; 心拍数; スペクトル分布; ゆらぎ; ニューロキニン

To establish a novel experimental model for vascular pain in guinea pigs, we have attempted to quantify emotional components of pain.1)It was demonstrated that intra-arterial injection of some algogenic substances induced vocalization response with increments of vocal sound's integrated value (VO) in a dose-dependent manner . 2) These VC values were markedly decreased by morphine and local anesthesia. 3) Intrathecal treatments of neurokinin A augmented capsaicin (CAP)-evoked vocalization responses. Furthermore, CAP-evoked vocalization responses were depressed by only tachykinin NK2 receptor antagonists. These results indicate that this model is a useful tool in the experimental model for vascular pain. In the next experiments, measurements of distress and discomfort using by cardiac R-R rhythms in this model. 1) Before and after the treatment of pain, R-R intervals of guinea pig's electrocardiogram exhibited a 1/f^b ffluctuation (b=1.7-2.8) in the power spectral density in the low frequency range (0.01-1Hz). 2) The spectrum slope in animals with suffering pain was more steeper than that in animals without suffering pain. These observations suggested that the property of the R-R interval variation has a fractal structure, and which may be used as a scale for measurements of distress and discomfort in the animal.

为了建立一种新的血管痛实验模型，我们尝试量化疼痛的情绪成分。1）实验证明，动脉内注射致痛物质可引起发声反应，并呈剂量依赖性地增加声音的积分值（VO）。2)这些VC值明显降低吗啡和局部麻醉。3)鞘内注射神经激肽A增强辣椒素（CAP）诱发的发声反应。此外，CAP诱发的发声反应只被速激肽NK 2受体拮抗剂抑制。这些结果表明，该模型是一个有用的工具，在血管疼痛的实验模型。在接下来的实验中，在该模型中使用心脏R-R节律测量痛苦和不适。1)在疼痛治疗前后，豚鼠心电图R-R间期在低频（0.01-1Hz）范围内，功率谱密度呈1/f^B波动（B=1.7-2.8）。2)有疼痛的动物比没有疼痛的动物的光谱斜率更陡。这些观察结果表明，R-R间期变化的性质具有分形结构，并可用作测量动物的痛苦和不适的尺度。

项目成果

安藤隆一郎,渡辺千寿子: "モルモット啼声反応による疼痛関連行動評価法" 日本薬理学雑誌. 112. 363-370 (1998)

安藤龙一郎、渡边千津子：“利用豚鼠哭泣反应评估疼痛相关行为的方法”《日本药理学杂志》112. 363-370 (1998)。

安藤隆一郎,渡辺千寿子: "モルモット啼声反応による疼痛関連評価法" 日本薬理学雑誌. 112. 363-370 (1998)

安藤龙一郎、渡边千津子：“利用豚鼠哭泣反应的疼痛相关评价方法”日本药理学杂志112. 363-370（1998）。

Ryuichiro, Ando Chizuko, Watanabe: "Vocalization response used for evaluation of pain-related behavior in guinea pigs." Folia Pharmacol.Jpn.112. 363-370 (1998)

Ryuichiro、Ando Chizuko、Watanabe：“用于评估豚鼠疼痛相关行为的发声反应。”