Study of marine carbon cycle based on stable carbon isotopic compositions of amino acids in marine sediments

基于海洋沉积物氨基酸稳定碳同位素组成的海洋碳循环研究

• 批准号: 22KJ0593
• 负责人: 孫 語辰
• 金额: $ 1.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ0593/
• 关键词: amino acid; marine sediment; microbial reworking; organic matter; diagenesis; carbon isotope

This research aims to elucidate the dynamics and mechanism of organic matter preservation and degradation in marine sediments, using carbon isotopic compositions of amino acids as the main tool.In this year, I further modified the analytical method for isotope analyses of amino acids in marine sediment samples. I applied a new HPLC column, Hypercarb column, for the separation of amino acids. Finally, I achieved the successful analyses of individual hydrophilic amino acids in marine sediments, which cannot be achieved by the previous method. I confirmed the analyses by testing amino acid standards. This method also shows the potential for other isotope analyses, such as nitrogen isotope and radiocarbon, which would benefit future researches on environmental amino acids.Furthermore, I developed a new method for the isolation of individual D-amino acids in environmental samples using a chiral HPLC column, and confirmed the reliability of stable carbon and nitrogen isotope analyses. This method can be applied to study the D-amino acids in marine sediments, which can be potentially used as a microbial biomarker to study the sources and transformations of organic matter under the sea floor in the future.

本研究以氨基酸的碳同位素组成为主要工具，旨在阐明海洋沉积物中有机质保存和降解的动力学和机制，本年度进一步改进了海洋沉积物样品中氨基酸的同位素分析方法。我应用了一种新的高效液相色谱柱，Hypercarb柱，用于分离氨基酸。最后，成功地实现了海洋沉积物中单个亲水性氨基酸的分析，这是以前的方法无法实现的。我通过测试氨基酸标准品证实了分析结果。此外，本文还建立了一种利用手性HPLC柱分离环境样品中单个D-氨基酸的新方法，证实了稳定碳、氮同位素分析的可靠性。该方法可应用于海洋沉积物中D-氨基酸的研究，并有望作为微生物生物标志物用于研究海底有机质的来源和转化。

项目成果

A new assessment of amino acid stable carbon isotope compositions in marine organisms: implications for food web studies

海洋生物中氨基酸稳定碳同位素组成的新评估：对食物网研究的影响

• 发表时间: 2021
• 作者: Yuchen Sun;Naoto F. Ishikawa;Nanako O. Ogawa;Yoshinori Takano;Naohiko Ohkouchi
• 通讯作者: Naohiko Ohkouchi

Integrative assessment for amino acid nitrogen isotopic composition in biological tissue samples determined by GC/C/IRMS, LC x EA/IRMS, and LC x GC/C/IRMS.

通过 GC/C/IRMS、LC x EA/IRMS 和 LC x GC/C/IRMS 测定生物组织样品中氨基酸氮同位素组成的综合评估。

• DOI: 10.1002/lom3.10502
• 发表时间: 2022
• 期刊: Limnology and Oceanography, Method
• 作者: Ishikawa;N. F.;Ogawa;N. O.;Sun;Y.;Chikaraishi;Y.;Takano;Y.;and Ohkouchi;N.
• 通讯作者: N.

Enantiomer-specific carbon isotope analysis of amino acids: New method and perspectives

氨基酸对映体特异性碳同位素分析：新方法和前景

• 发表时间: 2022
• 作者: Yuchen Sun;Thomas M. Blattmann;Naoto F. Ishikawa;Nanako O. Ogawa;Yoshinori Takano;Naohiko Ohkouchi
• 通讯作者: Naohiko Ohkouchi

Development and applications of a liquid chromatography method for the compound- and enantiomer-specific stable carbon isotope analysis of amino acids in environmental samples

用于环境样品中氨基酸的化合物和对映体特异性稳定碳同位素分析的液相色谱方法的开发和应用

• 发表时间: 2022
• 作者: Y. Sun;N. F. Ishikawa; N. O.O gawa;Y.Takano;N. Ohkouchi
• 通讯作者: N. Ohkouchi

Microbial Reworking of Organic Matter in Marine Sediments Revealed by Carbon Isotopic Compositions of Individual Amino Acids

单个氨基酸的碳同位素组成揭示了海洋沉积物中有机物的微生物再加工

• 发表时间: 2022
• 作者: Y. Sun;N. F. Ishikawa;N. O. Ogawa;J. Kuroda; Y.Takano;N. Ohkouchi
• 通讯作者: N. Ohkouchi