Relationship between retardation of auto immune disease

自身免疫性疾病迟缓的关系

• 批准号: 08680587
• 负责人: OOTSUYAMA Akira
• 金额: $ 1.54万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680587/
• 关键词: lpr mice; gld mice; gamma rays; spleen; thymus; T cell; Fas; Apoptosis; 1prマウス; FAS

In Makinodan's study (Makinodan, et al., 1990) , gamma rays of 0.04Gy or 0.1Gy were irradiated three times a week (total 20 times) in immune deficiency lpr mice. They reported that after the erradiation, the number of accumulated double-negative (CD4-/CD8-) T cells in the spleen was reduced.We analyzed whether there was a relationship between radiation sensitivity and apoptosis of the double-negative T cells accumulated in the spleen and thymus of the lpr and gld mice.The reduction of double-negative subpopulation of the spleen was statistically significant in MRL/Mpj-lpr/lpr mice with acute 1Gy gamma irradiation. In the protoractive irradiation group (total dose 0.98Gy) , statistical significance was not seen but the proportion of T cell subpopulations was improved.As any typical symptoms by immune-deficiency were not found in the study, using the C57BL/6J-lpr/lpr mice which were used by Makinodan, we could not observe the effects of irradiation on the mice.In the study with the MRN/MpTn-gld/gld mice, a reduction of double-negative subpopulation, and an increase of single-positive (CD4+/CD8-) subpopulation was observed in the spleen of the irradiation group. A reduction of the double-negative subpopulation and an increase of double-positive (CD4+/CD8+) subpopulation was observed in the thymus, but there was not statistical significance in ether result.Background and immune-deficiency conditions of the lpr and gld mice used for the experiments were different from Makinodan's. But, we found that the proportion of T cell subpopulations tended to improve. We guessed that the apoptosis occurred in the double-negative subpopulation with low dose radiation. We knew that the process of apoptosis-induced radiation did not need the Fas-FasL function.Retardation of disease progression by immune-deficiency of these mice might be possible with low dose radiation.

在Makinodan的研究中（Makinodan等人，1990），对免疫缺陷lpr小鼠进行0.04Gy或0.1Gy的γ射线照射，每周3次（共20次）。他们报告说，在核辐射之后，累计双负数结果表明，MRL/Mpj-lpr/gld小鼠脾脏和胸腺中的CD 4-/CD 8-T细胞亚群减少，脾脏中的CD 4-/CD 8-T细胞亚群减少，并与辐射敏感性有关，MRL/Mpj-lpr/gld小鼠脾脏和胸腺中的CD 4-/CD 8-T细胞亚群减少与辐射敏感性有关。lpr小鼠急性1Gy γ射线照射。质子放射组由于本研究中未发现任何典型的免疫缺陷症状，因此使用Makinodan使用的C57 BL/6 J-lpr/lpr小鼠，我们无法观察到辐射对小鼠的影响。在MRN/MpTn-gld/gld小鼠的研究中，照射组脾脏双阴性亚群减少，单阳性（CD 4 +/CD 8-）亚群增加。胸腺双阴性亚群减少，双阳性（CD_4 ~+/CD_8 ~+）亚群增加，但乙醚结果无统计学意义。但是，我们发现T细胞亚群的比例趋于改善。我们推测低剂量辐射引起的细胞凋亡发生在双阴性亚群中。我们知道，辐射诱导的肿瘤形成过程不需要Fas-FasL功能，低剂量辐射可能延缓免疫缺陷小鼠的疾病进展。