Relationship between retardation of auto immune disease

在Makinodan的研究中（Makinodan, et al., 1990），对免疫缺陷lpr小鼠每周照射3次（共20次）0.04Gy或0.1Gy的伽马射线。他们报告说，放射治疗后，脾脏中积累的双阴性（CD4-/CD8-） T细胞的数量减少。我们分析了lpr和gold小鼠的脾脏和胸腺中积累的双阴性T细胞的凋亡是否与辐射敏感性有关。急性1Gy γ辐射对MRL/Mpj-lpr/lpr小鼠脾脏双阴性亚群的减少有统计学意义。比例照射组（总剂量0.98Gy） T细胞亚群比例提高，但无统计学意义。由于研究中未发现任何典型的免疫缺陷症状，因此使用Makinodan使用的C57BL/6J-lpr/lpr小鼠，我们无法观察到辐照对小鼠的影响。在与MRN/ mpn -gld/gld小鼠的研究中，观察到照射组脾脏双阴性亚群减少，单阳性（CD4+/CD8-）亚群增加。胸腺双阴性亚群减少，双阳性（CD4+/CD8+）亚群增加，但两者结果均无统计学意义。实验用lpr小鼠和gold小鼠的背景和免疫缺陷情况与Makinodan小鼠不同。但是，我们发现T细胞亚群的比例趋于改善。我们推测细胞凋亡发生在低剂量辐射下的双阴性亚群中。我们知道凋亡诱导辐射的过程不需要Fas-FasL功能。低剂量辐射可能延缓这些小鼠免疫缺陷的疾病进展。

In Makinodan's study (Makinodan, et al., 1990) , gamma rays of 0.04Gy or 0.1Gy were irradiated three times a week (total 20 times) in immune deficiency lpr mice. They reported that after the erradiation, the number of accumulated double-negative (CD4-/CD8-) T cells in the spleen was reduced.We analyzed whether there was a relationship between radiation sensitivity and apoptosis of the double-negative T cells accumulated in the spleen and thymus of the lpr and gld mice.The reduction of double-negative subpopulation of the spleen was statistically significant in MRL/Mpj-lpr/lpr mice with acute 1Gy gamma irradiation. In the protoractive irradiation group (total dose 0.98Gy) , statistical significance was not seen but the proportion of T cell subpopulations was improved.As any typical symptoms by immune-deficiency were not found in the study, using the C57BL/6J-lpr/lpr mice which were used by Makinodan, we could not observe the effects of irradiation on the mice.In the study with the MRN/MpTn-gld/gld mice, a reduction of double-negative subpopulation, and an increase of single-positive (CD4+/CD8-) subpopulation was observed in the spleen of the irradiation group. A reduction of the double-negative subpopulation and an increase of double-positive (CD4+/CD8+) subpopulation was observed in the thymus, but there was not statistical significance in ether result.Background and immune-deficiency conditions of the lpr and gld mice used for the experiments were different from Makinodan's. But, we found that the proportion of T cell subpopulations tended to improve. We guessed that the apoptosis occurred in the double-negative subpopulation with low dose radiation. We knew that the process of apoptosis-induced radiation did not need the Fas-FasL function.Retardation of disease progression by immune-deficiency of these mice might be possible with low dose radiation.