Three-dimensional image analysis of migration patterns of mouse neural crest cells

小鼠神经嵴细胞迁移模式的三维图像分析

• 批准号: 08680791
• 负责人: ITO Kazuo
• 金额: $ 1.6万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680791/
• 关键词: mouse neural crest; migration pattern; three-dimensional image analysis; extracelluar matrix

We have examined migration patterns of mouse neural crest cells by three-dimensional image analysis using the monoclonal antibody 4E9R to identify neural crest cells. In the trunk, three sclerotomal regions having different migratory pathways of neural crest cells were identified along the anteroposterior axis and the size of these regions temporally changed with a systematic mode. The data suggest that the regional differences of neural crest cell migration within each sclerotome and their temporal changes generate migration patterns of trunk neural crest cells in the mouse. The distribution of chondroitin sulfate proteoglycans in the scletotome was correlated with migration patterns of neural crest cells. Furthermore, their migration patterns were altered in embryos treated with the inhibitors of sulfated proteoglycan biosynthesis, sodium chlorate and beta-D-xyloside. We conclude that systematic spatiotemporally changes of the distribution of chondroitin sulfate proteoglycans in the sclerotome are a key requisite for the formation of migration patterns of mouse trunk neural crest cells. The role of tenascin in neural crest cell migration has been controversial. Migration patterns of these cells in tenascin-knockout mouse embryos were comparable with those of wild-type crest cells. This support the notion that tenascin is not directly responsible for the segmented outgrowth of trunk neural crest cells. In the cephalic region, mesencephalic neural crest cells migrated in the specific portion, that is in the mesenchyme proximal to the boundary between the midbrain and the hindbrain.

我们使用单克隆抗体 4E9R 通过三维图像分析检查了小鼠神经嵴细胞的迁移模式，以识别神经嵴细胞。在躯干中，沿前后轴识别出具有不同神经嵴细胞迁移途径的三个巩膜区域，并且这些区域的大小随系统模式随时间变化。数据表明，每个骨节内神经嵴细胞迁移的区域差异及其时间变化产生了小鼠躯干神经嵴细胞的迁移模式。骨片中硫酸软骨素蛋白多糖的分布与神经嵴细胞的迁移模式相关。此外，在用硫酸化蛋白聚糖生物合成抑制剂、氯酸钠和β-D-木糖苷处理的胚胎中，它们的迁移模式发生了改变。我们得出的结论是，骨节中硫酸软骨素蛋白聚糖分布的系统时空变化是小鼠躯干神经嵴细胞迁移模式形成的关键必要条件。生腱蛋白在神经嵴细胞迁移中的作用一直存在争议。这些细胞在生腱蛋白敲除小鼠胚胎中的迁移模式与野生型嵴细胞的迁移模式相当。这支持了腱蛋白不直接负责躯干神经嵴细胞的分段生长的观点。在头部区域，中脑神经嵴细胞迁移到特定部分，即靠近中脑和后脑之间边界的间充质中。