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Fundamental Research for the synthesis of biorecognition glycosides by enzymatic introduction of oligosaccharides from glycoproteins

Fundamental Research for the synthesis of biorecognition glycosides by enzymatic introduction of oligosaccharides from glycoproteins
糖蛋白酶促引入寡糖合成生物识别糖苷的基础研究
批准号:
21580117
负责人:
ITO Kazuo
金额:
$3.16万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011

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中文摘要
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英文摘要
Expression system and purification method of recombinant N-linked oligosaccharide-releasing enzyme was established. The recombinant enzyme released oligosaccharides from various glycoproteins. Furthermore, it transferred the released oligosaccharides to form potential bio-recognition glycosides. Analysis of mutant recombinant enzyme showed the functional area on the enzyme. Furthermore, Crystallization of the recombinant enzyme succeeded for X-ray structural analysis.
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Asparagine-linked oligosaccharide-releasing enzymes produced by basidiomycetes
担子菌产生的天冬酰胺连接的寡糖释放酶
DOI: --
发表时间: 2011
期刊: Oyotositukagaku
影响因子: --
作者: [T.Hamaguchi, et al]
通讯作者: et al
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [海住宜広, 濱口祐, 久田博元, 秦洋二, Kuakarun Krusong, Jarunee Kaulpiboon, Tipaporn Limpaseni, Piamsook Pongsawasdi, 伊藤和央]
通讯作者: 伊藤和央
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [浜口祐, 海住宜広, 篠原かな子, Kuakarn Kursong, Jarnee Kaurpiborn, Tipaporn Limpaseni, Piamsook Pongsawasdi, 伊藤和央, 浜口祐, 海住宜広]
通讯作者: 海住宜広
担子菌Agaricus bisporus由来エンド-β-N-アセチルグルコサミニダーゼABの精製とその性質
担子菌双孢蘑菇内切-β-N-乙酰氨基葡萄糖苷酶 AB 的纯化及性质
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [谷脇聡, 濱口祐, Kuakarun Krusong, Jarunee Kaulpiboon, Tipaporn Limpaseni, PiamsookPongsawasdi, 伊藤和央, 谷脇聡]
通讯作者: 谷脇聡
44
    Expression of catabolic pathway of asparagine-linked oligosaccharides on plasma membrane and apoptosis
    • 批准号:
      14580628
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.24万
    • 财政年份:
      2002
    • 负责人:
      ITO Kazuo
    • 依托单位:
    Molecular Evolution of Structure and Specificity of Asparagine-Linked Oligosaccharide Releasing Enzyme
    • 批准号:
      08680662
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $1.47万
    • 财政年份:
      1996
    • 负责人:
      ITO Kazuo
    • 依托单位:
    Three-dimensional image analysis of migration patterns of mouse neural crest cells
    • 批准号:
      08680791
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $1.6万
    • 财政年份:
      1996
    • 负责人:
      ITO Kazuo
    • 依托单位:
    海外基金