Anti-tumor Effect of Immunity treatment on the Growth of VX2 Carcinoma Transplanted in the Tongue of Rabitts

我们对兔VX_2舌癌进行了BRM与多种抑癌剂联合给药和联合给药的实验研究。本实验将LNT和CDGP分别作用于兔VX 2舌癌组织，观察LNT和CDGP对VX 2舌癌细胞凋亡的诱导作用，并采用TUNEL法进行染色，结果显示：TUNEL阳性细胞的细胞核被TACS Blue Label染成蓝绿色<TM>;肿瘤中心部分组织丢失，坏死，近边缘部分可见TUNEL阳性细胞。LNT给药组和CDGP给药组的细胞凋亡诱导有意地增强到对照组。LNT与CDGP合用组进一步促进细胞凋亡的诱导。在CDGP给药组中，肿瘤和静脉给药组中，通过给药途径诱导的细胞凋亡未见显著差异。CDGP和CDDP诱导细胞凋亡的作用无显著性差异，因此有必要阐明其凋亡调控因子，并建议将其作为提高实体癌化疗效果的因素之一。

We have done administering and administering of using together of a medicine made of BRM and various carcinostatic agent to the VX2 carcinoma tongue of the rabbit. We administered LNT and CDGP to the VX2 carcinoma tongue of the rabbit to evaluate the apoptosis inducement ability of LNT and CDGP, and dyed by the TUNEL method at this time.The following results were obtained.The nucleus of the TUNHL positivity cell was dyed to blue green with TACS^<TM> Blue Label. The loss of the organization with the necrosis was observed in the center part of the tumor, and the TUNEL positivity cell was admitted in the near edge part of the tumor. The apoptosis inducement has intentionally reinforced both LNT administering groups and the CDGP administering groups to the Control group. The inducement of apoptosis was promoted further in LNT and the administering CDGP using together group. In the administering group of CDGP, in the administering group in the tumor and the vein, a significant difference was not seen by the apoptosis inducement by the adninistering route. A significant difference was not admitted in the apoptosis inducement between CDGP and CDDP.The elucidation of the apoptosis control factor was necessary, and it was suggested to become an element by which it improved taking effect of chemotherapy to a solid carcinona.