Anti-tumor Effect of Immunity treatment on the Growth of VX2 Carcinoma Transplanted in the Tongue of Rabitts

免疫治疗对兔舌VX2癌移植瘤生长的抗肿瘤作用

• 批准号: 09672042
• 负责人: TATEMATSU Norichika
• 金额: $ 0.96万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672042/
• 关键词: VX2; BRM; Lentinan; CDDP; CDGP; TUNEL; Apoptosis; VX-2; 5-FU; BRM製剤; 実験腫瘍

We have done administering and administering of using together of a medicine made of BRM and various carcinostatic agent to the VX2 carcinoma tongue of the rabbit. We administered LNT and CDGP to the VX2 carcinoma tongue of the rabbit to evaluate the apoptosis inducement ability of LNT and CDGP, and dyed by the TUNEL method at this time.The following results were obtained.The nucleus of the TUNHL positivity cell was dyed to blue green with TACS^<TM> Blue Label. The loss of the organization with the necrosis was observed in the center part of the tumor, and the TUNEL positivity cell was admitted in the near edge part of the tumor. The apoptosis inducement has intentionally reinforced both LNT administering groups and the CDGP administering groups to the Control group. The inducement of apoptosis was promoted further in LNT and the administering CDGP using together group. In the administering group of CDGP, in the administering group in the tumor and the vein, a significant difference was not seen by the apoptosis inducement by the adninistering route. A significant difference was not admitted in the apoptosis inducement between CDGP and CDDP.The elucidation of the apoptosis control factor was necessary, and it was suggested to become an element by which it improved taking effect of chemotherapy to a solid carcinona.

我们对兔VX2癌舌进行了BRM与多种抗癌剂联合用药和给药。我们将LNT和CDGP分别用于兔VX2癌舌，观察LNT和CDGP诱导兔VX2癌舌细胞凋亡的能力，并进行TUNEL染色。得到了以下结果：用TACS^<TM>蓝标将TUNHL阳性细胞细胞核染成蓝绿色。肿瘤中心部分组织丢失伴坏死，肿瘤近边缘部分出现TUNEL阳性细胞。凋亡诱导作用有意增强LNT给药组和CDGP给药组对对照组的影响。LNT与CDGP联用组对细胞凋亡的诱导作用进一步增强。CDGP给药组、肿瘤给药组和静脉给药组，不同给药途径诱导细胞凋亡的差异无统计学意义。CDGP和CDDP在诱导细胞凋亡方面无明显差异。细胞凋亡控制因子的阐明是必要的，并被认为是提高化疗对实体癌疗效的一个因素。

项目成果

安田 聡、藤塚秀樹、牧田浩樹、宮本 謙、市原秀記、立松憲親: "家兎VX2舌癌に対するレンチナンと各種抗癌剤併用の抗腫瘍効果について(第1報)シスプラチンとの併用、TUNEL法による抗腫瘍効果の検討"岐阜大学医学部紀要. 47・6. 209-214 (1999)

Satoshi Yasuda、Hideki Fujitsuka、Hiroki Makita、Ken Miyamoto、Hideki Ichihara、Norichika Tatematsu：“香菇多糖联合多种抗癌药物对兔 VX2 舌癌的抗肿瘤作用（首次报道）与顺铂和 TUNEL 联合的抗肿瘤作用肿瘤效果的检查”岐阜大学医学部学报47・6（1999）。

Satoshi Y, Makoto T and Norichka T: "Anti-Tumor Effect of Combination of Lentinan and Carcinostatic Agents on the Growth of VX2 Carcinoma Transplated in the Tongue of Rabbits(II)"Acta Scholae Medichinalis Universitatis In Gifu. 48. 255-260 (2000)

Satoshi Y、Makoto T 和 Norichka T：“香菇多糖和抗癌剂组合对兔舌中移植的 VX2 癌生长的抗肿瘤作用（II）”岐阜大学医学学报。

安田聡,藤塚秀樹,牧田浩樹,宮本謙,市原秀記,立松憲親: "家兎VX2舌癌に対するレンチナンと各種抗癌剤併用の抗腫瘍効果について(第1報)シスプラチンとの併用,TUNEL法による抗腫瘍効果の検討"岐阜大学医学部紀要. 47・6. 209-214 (1999)

Satoshi Yasuda、Hideki Fujitsuka、Hiroki Makita、Ken Miyamoto、Hideki Ichihara、Norichika Tatematsu：“香菇多糖与各种抗癌药物联合使用对兔 VX2 舌癌的抗肿瘤作用（第 1 次报告）与顺铂联合使用，使用 TUNEL 的抗肿瘤作用方法“肿瘤效果检查”岐阜大学医学部学报.47・6.209-214(1999)

安田聡・藤塚秀樹・牧田浩樹・宮本謙・市原秀記・立松憲親: "家兎VX2舌癌に対するレンチナンと各種抗癌剤併用の抗腫瘍効果について(第1報)シスプラチンとの併用,TUNEL法による抗腫瘍効果の検討"岐阜大学医学部紀要. 47. 209-214 (1999)

Satoshi Yasuda、Hideki Fujitsuka、Hiroki Makita、Ken Miyamoto、Hideki Ichihara、Norichika Tatematsu：“香菇多糖联合多种抗癌药物对兔 VX2 舌癌的抗肿瘤作用（首次报道）与顺铂和 TUNEL 联合的抗肿瘤作用肿瘤效果的检查方法” 岐阜大学医学部学报. 47. 209-214 (1999)

安田聡・土井田誠・立松憲親: "家兎VX2舌癌に対するレンチナンと各種抗癌剤併用の抗腫瘍効果について(II)ネダプラチンとの併用,TUNEL法による抗腫瘍効果の検討"岐阜大学医学部紀要. 48. 255-260 (2000)

Satoshi Yasuda、Makoto Doida、Norichika Tatematsu：《论香菇多糖与各种抗癌药物联用对兔 VX2 舌癌的抗肿瘤作用（二）与奈达铂联用和 TUNEL 法的抗肿瘤效果检验》岐阜大学医学院通报48.255-260（2000）