Histological study of pathological root resorption in human deciduous teeth with apical periodontitis.

人乳牙根尖周炎病理性牙根吸收的组织学研究。

• 批准号: 09672089
• 负责人: YAWAKA Yasutaka
• 金额: $ 2.24万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672089/
• 关键词: human deciduous teeth; apical periodontitis; pathologlcal root resorption; cementum deposition; cultured osteoclasts; tartrate-resistant acid phosphatase (TRAP); enbedding; histologicai observation; 走査型電子顕微鏡; 酒石産耐性酸フォスファターゼ(TRAP); 酒石酸耐性酸フォスファターゼ

The purpose of this study was to examine a different root resorption process on the root dentin with apical periodontitis or on sound root dentin.1. The region of the root apex with severe apical periodontitis in human deciduous teeth was observed by light and transmission electron microscopy. Root dentin and original cementum had a severe irregular caved surface due to pathological root resorption. Cementum had partial!y deposited on the caved dentin and original cementum surfaces. The structure of deposited cementum was very different from that of the original cementum. It was thought that partial repair mechanisms such as cementum deposition are affected by the surrounding environment of the root.2. Root dentin slices were prepared from human deciduous teeth with apical periodontitis (group p) or sound human deciduous teeth (group n, control). Cell suspension of bone marrow from the femur of mice in α MEM was seeded on the dentin slices and incubated. After incubation, specimens wer … More e removed and fixed, and then observed for tartrate-resistant acid phosphatase (TRAP) activity. The number of TRAP-positive cells on the dentin was calculated by light microscopic observation. TRAP-positive cells were observed in all dentin slices. There were no significant differences between group p and group n on the number of TRAP-positive cells by Mann-Whitney test. The results suggested that root dentin with apical periodontitis was resorbed by cultured osteoclasts It was thought that enviromental factors in root circumferences had a greater effect than does the condition of root dentin on pathological root resorption.3. The root dentin slices of group p and group n were embedded in rats subcutaneous tissue. The dentin slices of both groups were surrounded by fibrous connective tissue are inflammatory cells and partially resorbed by multinuclear giant cells. However, the resorption was slight. There were no differences between the both groups on the condition of the dentin slices. It was thought that the condition of root dentin had few effect on pathological root resorption.Therefore, it was suggested that enviromental factors in root circumferences had a great effect on patholagical root resorption. Less

本研究的目的是探讨根尖周炎和健康根面牙本质的不同牙根吸收过程.应用光镜和透射电镜对人乳牙根尖重度根尖周炎区进行了观察。由于病理性牙根吸收，牙根牙本质和原始牙骨质表面严重不规则凹陷。牙骨质有部分！Y沉积在凹陷的牙本质和原始牙骨质表面上。沉积牙骨质的结构与原始牙骨质有很大的不同。部分修复机制如牙骨质沉积被认为受到牙根周围环境的影响.制备根尖周炎乳牙（p组）和健康乳牙（n组，对照）的根面牙本质切片。将小鼠股骨骨髓细胞悬液接种于牙本质切片上进行培养。孵育后， ...更多信息 e取出并固定，然后观察抗酒石酸酸性磷酸酶（TRAP）活性。光镜下观察牙本质表面TRAP阳性细胞数。TRAP阳性细胞在所有牙本质切片中观察到。经Mann-Whitney检验，p组和n组TRAP阳性细胞数无显著差异。结果提示根尖周炎的根面牙本质被破骨细胞吸收，根周环境因素比根面牙本质状况对病理性根面吸收的影响更大.将p组和n组的根面牙本质切片包埋于大鼠皮下组织中。两组牙本质切片均被纤维结缔组织、炎性细胞包围，部分被多核巨细胞吸收。但是，吸收轻微。两组牙本质切片情况无差异。认为根面牙本质的状况对病理性根吸收的影响不大，提示根周环境因素对病理性根吸收有重要影响。少