Histological study of pathological root resorption in human deciduous teeth with apical periodontitis.

本研究的目的是观察患根尖周炎的根面牙本质和健康根面牙本质上不同的牙根吸收过程。对严重根尖周炎的人乳牙根尖区进行了光镜和电子显微镜观察。由于病理性的牙根吸收，根牙本质和原始牙骨质有严重的不规则凹陷。牙骨质部分沉积在凹陷的牙本质和原始牙骨质表面。沉积牙骨质的结构与原始牙骨质有很大的不同。认为牙骨质沉积等局部修复机制受牙根周围环境的影响。分别从患根尖周炎的人乳牙(p组)和健康的人乳牙(n组，对照组)制备根面牙本质切片。将取自小鼠股骨的骨髓细胞悬液接种于α基质细胞悬液上，在牙本质切片上培养。孵化后的标本为…。去除和固定更多的E，然后观察抗酒石酸酸性磷酸酶(TRAP)活性。光镜观察计算牙本质表面TRAP阳性细胞数。所有牙本质切片中均可见TRAP阳性细胞。经Mann-Whitney检验，p组与n组之间TRAP阳性细胞数差异无统计学意义。结果提示，根尖周炎患者的根面牙本质被体外培养的破骨细胞吸收，认为牙根周围环境因素对病理性牙根吸收的影响大于根面状况。将p组和n组的根部牙本质切片包埋于大鼠皮下组织中。两组牙本质切片均被纤维结缔组织包裹，均为炎性细胞，部分被多核巨细胞吸收。然而，这种吸收是轻微的。两组间牙本质切片情况无明显差异。认为根面牙本质状态对病理性牙根吸收影响不大，提示牙根周围环境因素对病理性牙根吸收有较大影响。较少

The purpose of this study was to examine a different root resorption process on the root dentin with apical periodontitis or on sound root dentin.1. The region of the root apex with severe apical periodontitis in human deciduous teeth was observed by light and transmission electron microscopy. Root dentin and original cementum had a severe irregular caved surface due to pathological root resorption. Cementum had partial!y deposited on the caved dentin and original cementum surfaces. The structure of deposited cementum was very different from that of the original cementum. It was thought that partial repair mechanisms such as cementum deposition are affected by the surrounding environment of the root.2. Root dentin slices were prepared from human deciduous teeth with apical periodontitis (group p) or sound human deciduous teeth (group n, control). Cell suspension of bone marrow from the femur of mice in α MEM was seeded on the dentin slices and incubated. After incubation, specimens wer … More e removed and fixed, and then observed for tartrate-resistant acid phosphatase (TRAP) activity. The number of TRAP-positive cells on the dentin was calculated by light microscopic observation. TRAP-positive cells were observed in all dentin slices. There were no significant differences between group p and group n on the number of TRAP-positive cells by Mann-Whitney test. The results suggested that root dentin with apical periodontitis was resorbed by cultured osteoclasts It was thought that enviromental factors in root circumferences had a greater effect than does the condition of root dentin on pathological root resorption.3. The root dentin slices of group p and group n were embedded in rats subcutaneous tissue. The dentin slices of both groups were surrounded by fibrous connective tissue are inflammatory cells and partially resorbed by multinuclear giant cells. However, the resorption was slight. There were no differences between the both groups on the condition of the dentin slices. It was thought that the condition of root dentin had few effect on pathological root resorption.Therefore, it was suggested that enviromental factors in root circumferences had a great effect on patholagical root resorption. Less