The Structural Studies of Interaction between Polyamine and Z-DNA

多胺与Z-DNA相互作用的结构研究

• 批准号: 09672201
• 负责人: OHISHI Hirofumi
• 金额: $ 1.6万
• 依托单位: Osaka University of Pharmaceutical Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672201/
• 关键词: left handed Z-DNA; Polyamine; minor groove; X-ray crystallography; Molecular Dynamics; salt concentration; interaction; molecular design; 核酸; 安定化機構; コンピューター解析; ポリアミン; 金属カウンターイオン; Z-DNA; B-Z転移; 構造科学; 遺伝子発現制御機構; 発癌機構

It was proven that the Z-DNA recognition protein made X-ray and crystal structure analysis in the complex crystal with Z-DNA in 1999 and that the protein is RNAaminase. It was proven that this protein was the enzyme which converts the guanine base of nucleic acid base into the adenine base. It has also been proven that there is the work that it causes the transfer abnormality in order to convert into the base that it converts the guanine bases of nucleic acid into the adenine base by it and is originally different from the base code of the protein as a purpose, and grows the protein of the purpose outside. Polyamine and mystery of Z-DNA are deepening more with the clarification of such fact. It has also been clarified that DNA becomes counterclockwise Z-DNA, when high-dense Polyamine is mixed with DNA. Then, it succeeded in the design of Polyamine which stabilizes Z-DNA we on clarifying various Polyamine and Z-DNA complex crystal structure in order to examine interaction mode of variou … More s Polyamine and DNA, and on clarifying the action for Z-DNA of Polyamine. Then, further we design these Polyamine, and in addition, complex crystal between Polyamine and Z-DNA which seem to powerfully stabilize Z-DNA is made, and X-ray crystal structure analysis will be done. In addition, sequence and relevance to Z-DNA will be clarified using X-ray crystal structure analysis method, since that the sequence dependence is in the possibility of Z-DNA has also been proven. It has been proven that it is stabilized with in computer science research using molecular dynamics method, it stabilizes Z-DNA by metal ion, and in addition, the metallic mass increases. When the Polyamine exist together with metal counter ion, as the concentration of the metal ions is low level the Z-DNA is stabilized. However, it was also proven that Z-DNA could not be stabilized in the Polyamine simple substance. In addition, that it did not become Z-DNA even in what kind of condition by the molecular dynamics calculation in (d(AC)ィイD24ィエD2/d(TG)ィイD24ィエD2) sequence clarified. Less

1999年通过对Z-DNA识别蛋白与Z-DNA的复合物晶体进行X射线衍射和晶体结构分析，证明该蛋白为RNA酶。证明该蛋白质是将核酸碱基中的鸟嘌呤碱基转化为腺嘌呤碱基的酶。还已经证明，存在其引起转移异常以转化为碱基的工作，其通过其将核酸的鸟嘌呤碱基转化为腺嘌呤碱基，并且最初不同于作为目的的蛋白质的碱基代码，并且在外部生长目的的蛋白质。随着这一事实的澄清，多胺和Z-DNA的神秘性更加加深。也已经阐明，当高密度聚胺与DNA混合时，DNA变成逆时针Z-DNA。然后，通过对各种多胺与Z-DNA复合物晶体结构的研究，成功地设计了稳定Z-DNA的多胺，以考察各种多胺与Z-DNA复合物的相互作用方式， ...更多信息 多胺与DNA的关系，阐明了多胺对Z-DNA的作用。然后，我们进一步设计了这些多胺，并制备了多胺和Z-DNA之间的复合晶体，这些复合晶体似乎有力地稳定了Z-DNA，并进行了X射线晶体结构分析。此外，将使用X射线晶体结构分析方法阐明序列和与Z-DNA的相关性，因为序列依赖性也已被证明是Z-DNA的可能性。在计算机科学研究中，使用分子动力学方法已经证明它是稳定的，它通过金属离子稳定Z-DNA，此外，金属质量增加。当多胺与金属反离子共存时，由于金属离子的浓度较低，所以Z-DNA是稳定的。然而，也证明了Z-DNA不能在多胺简单物质中稳定。另外，通过（d（AC）Δ D24 Δ D2/d（TG）Δ D24 Δ D2）序列的分子动力学计算，明确了在何种条件下也没有成为Z-DNA。少

项目成果

Hirofumi Ohishi, Noriko Terasoma, Isao Nakanishi, Gijs van der Marel, Jacques H. van Boom, Alexander Rich, Andrew H. J. Wang, Toshio Hakoshima, and Ken-ichi Tomita: "Interaction between the left-handed Z-DNA and Polyamine-3 : The crystal structure of the

Hirofumi Ohishi、Noriko Terasoma、Isao Nakanishi、Gijs van der Marel、Jacques H. van Boom、Alexander Rich、Andrew H. J. Wang、Toshio Hakoshima 和 Ken-ichi Tomita：“左手 Z-DNA 与 Polyamine-3 之间的相互作用

Ken-ichi Tomita: "Crystal Structure of a Left-handed Z-DNA Hexamer, d(CG)_3 Duplex Complexed with Synthetic Polyamine Binding of a Polyamine in the Minor Groove at Room Temperature"Nucleoside and Nucleotide. 18. 1567-1569 (1999)

Ken-ichi Tomita：“左手 Z-DNA 六聚体的晶体结构，d(CG)_3 双链体与室温下小凹槽中多胺的合成多胺结合”核苷和核苷酸。

Hirofumi Ohishi: "Comparison of a Left-Handed Z-DNA Molecular Structure Determined by X-Rays with That Simulated by a Molecular Dynamics" Biochemical and Biophysical Research Communications. 236. 146-150 (1997)

Hirofumi Ohishi：“X 射线确定的左手 Z-DNA 分子结构与分子动力学模拟的比较”生物化学和生物物理研究通讯。

Hirofumi Ohishi: "The Computation Studies on the Conformation-stabilizing Factors of the Left-handed Z-DNA"Nucleoside and Nucleotide. 18. 1571-1573 (1999)

Hirofumi Ohishi：“左手 Z-DNA 构象稳定因子的计算研究”核苷和核苷酸。

Hirofumi Ohishi, Isao Nakanishi, Ken-ichi Tomita, and Toshimasa Ishida: "The Computation Studies on the Conformation-stabilizing Factors of the Left-handed Z-DNA"Nucleoside and Nucleotide. 18. 1571-1573 (1999)

Hirofumi Ohishi、Isao Nakanishi、Ken-ichi Tomita 和 Toshimasa Ishida：“左手 Z-DNA 构象稳定因子的计算研究”核苷和核苷酸。