A structure scientific study about interaction of polyamine and left-handed DNA.

关于多胺与左手DNA相互作用的结构科学研究。

• 批准号: 15590051
• 负责人: OHISHI Hirofumi
• 金额: $ 1.98万
• 依托单位: Osaka University of Pharmaceutical Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590051/
• 关键词: X-ray crystallography; Z-DNA; B-Z junction; B-Z transfer; minor groove; groove binder; B-Z combination crystal; minor groove cavity; DNA; ポリアミン; モノアミン; コンフォメーション; 左巻きDNA; Z-型DNA; B-Z転移; ポリアミンナノワイアー; 相互作用; 結晶構造解析; 三重螺旋; 転写; 高塩濃度; スーパコイ

I understood that N1-(2-(2-aminoethylamino)ethyl)ethane-1,2-diamine PA (222) and N1-(2-(2-(2-aminoethylamino)ethylamino)ethyl)ethane-1,2-diamine PA (2222) were easy to be connected in a minor groove of Z-DNA very much and stabilized Z-DNA as a minor groove binder very much. Furthermore, I crystallize at 4 degrees Celsius, 10 degrees Celsius, 20 degrees Celsius by the high salt density with these 11 kinds of polyamine and d(CG)3 as a complex and give the salt density at 20 degrees Celsius very much and crystallize. I examine a role for Z-DNA of polyamine, stabilization action in detail by examining these. I perform crystallization of a long chain of Z-DNA which it has been said to that the above-mentioned crystallization vs. eight bases is difficult till now if I understand stabilization mechanism. I think to clarify structure of DNA such as mixture type d(CGCGCGCGCGAATTCGCGCGCGCG)2, d(CGCGCGCGAATTCGCGCGCG)2 and d(CGCGCGAATTCGCGCG)2 of B type DNA and Z type DNA to clarify B-Z transfer m … More echanism, Z-B transfer mechanism. As for the structure of DNA of B-Z mixture type, crystallization is tried all over the world till now, but there is not a report besides an example of our crystallization. I think about letting we bury a cavity of the big minor groove that it is it in an element of destabilization with this polyamine with the polyamine which is a minor groove binder as DNA and a complex of these long chains and stabilize structure of the whole DNA. Therefore we succeed in X-ray crystallographic analysis of left-handed DNA of a long chain. As for the structure of DNA of B-Z mixture type, crystallization is tried all over the world till now, but there is not a report besides an example of our crystallization. The groove binder polyamine which it is crawled, and I discovered is connected in a minor groove of just Z-DNA and I bury a big minor groove and stabilize structure of the whole DNA. I apply this and perform crystallization of DNA of a long chain, crystallization of B-Z-B junction structure. I think that I bring big development in structured study of DNA if I have already succeeded in crystallization of the DNA which is the longest in the world with this method now, and structure or B-Z-B junction structure of DNA of a long chain are clarified in sequence Less

我了解到N1-（2-（2-氨基乙氨基）乙基）乙烷-1，2-二胺PA（222）和N1-（2-（2-（2-氨基乙氨基）乙基）乙烷-1，2-二胺PA（2222）非常容易连接在Z-DNA的小沟中，并且非常稳定Z-DNA作为小沟结合剂。此外，我以这11种多胺和d（CG）3作为络合物在4 ℃、10 ℃、20 ℃以高盐密度结晶，在20 ℃以高盐密度结晶。我检查了多胺的Z-DNA的作用，通过检查这些详细的稳定作用。我进行了Z-DNA长链的结晶，如果我了解稳定机制，那么上述结晶与八个碱基相比到目前为止是困难的。我认为要弄清DNA的结构，如B型DNA和Z型DNA的混合型d（CGCGCGCGCGAATTCGCGCGCG）2、d（CGCGCGCGAATTCGCGCG）2和d（CGCGCGAATTCGCGCG）2， ...更多信息 机构、Z-B转移机构。对于B-Z混合型DNA的结构，迄今为止，世界各国都在进行结晶化的尝试，但除了我们的一个结晶化实例外，还没有报道。我考虑让我们埋下一个大小沟的空腔，它是这种多胺的不稳定元素，多胺是小沟结合剂，作为DNA和这些长链的复合物，并稳定整个DNA的结构。因此，我们成功地在X射线晶体学分析的左手DNA的长链。对于B-Z混合型DNA的结构，迄今为止，世界各国都在进行结晶化的尝试，但除了我们的一个结晶化实例外，还没有报道。我发现它所爬行的多胺结合在一个小沟中，我埋了一个大的小沟，稳定了整个DNA的结构。我应用这个并执行长链DNA的结晶，B-Z-B结结构的结晶。我认为，如果我已经成功地用这种方法结晶了目前世界上最长的DNA，并按顺序阐明了长链DNA的结构或B-Z-B连接结构，我将在DNA的结构化研究中带来巨大的发展。

项目成果

Intermolecular association model of antifungal polyene macrolide via concerted =C-H… O interactions : X-ray crystal structure analysis and ab initio molecular-orbital calculations of chainin.

通过协调 =C-H…O 相互作用的抗真菌多烯大环内酯的分子间缔合模型：X 射线晶体结构分析和 chainin 的从头算分子轨道计算。

• 发表时间: 2006
• 期刊: Bulletin of the Chemical Society of Japan 79
• 作者: In;Yasuko;Ohishi;Hirofumi;Miyagawa;Hiroo;Kitamura;Kunihiro;Igarashi;Yasuhiro;Ishida;Toshimasa.
• 通讯作者: Toshimasa.

計算機科学を用いたDNAの創薬化学

利用计算机科学进行 DNA 药物化学

• 发表时间: 2006
• 期刊: 生産と技術 印刷中
• 作者: 大石宏文

Stabilization of the left-handed Z-DNA with monoamines and polyamines

用单胺和多胺稳定左手 Z-DNA

• 发表时间: 2005
• 期刊: Nucleic Acids Research Supplement
• 作者: Koji Tsukamoto;Mamiko Odoko;Hirofumi Ohishi;Yoichi Hiyama;Naoyoshi Maezaki;Nobuo Okabe;Tetsuaki Tanaka;Toshimasa Ishida
• 通讯作者: Toshimasa Ishida

Asymmetric Intramolecular Michael Addition of α-Sulfinyl Vinylic Carbanion to Enoates.

α-亚磺酰乙烯基碳负离子与 Enoates 的不对称分子内迈克尔加成。

• 发表时间: 2004
• 期刊: Journal of Organic Chemistry 69
• 作者: Maezaki;Naoyoshi;Sawamoto;Hiroaki;Yuyama;Sachiko;Yoshigami;Ryoko;Suzuki;Tomoko;Izumi;Mayuko;Ohishi;Hirofumi;Tanaka;Tetsuaki.
• 通讯作者: Tetsuaki.

Antineoplastic agents.: 551.: Isolation and structures of bauhiniastatins 1-4 from Bauhinia purpurea

• DOI: 10.1021/np058075
• 发表时间: 2006-03-01
• 期刊: JOURNAL OF NATURAL PRODUCTS
• 影响因子: 5.1
• 作者: Pettit, GR;Numata, A;Cragg, GM
• 通讯作者: Cragg, GM