CELL-CYCLE DEPENDENT REGULATION OF HSP70 GENE EXPRESSION BY C-MYC
C-MYC 对 HSP70 基因表达的细胞周期依赖性调控
基本信息
- 批准号:09672207
- 负责人:
- 金额:$ 1.6万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have previously identified at about -150 from the transcriptional initiation site in the human hsp70 promoter the HSP-MYC-B element which is recognized by a c-Myc protein complex and contributes to the early G1 specific expression of the gene. To identify the proteins involved in the c-Myc complex formed on the HSP-MYC-B and contributes the G1 specific expression, the one-hybrid screening in yeast targetin the HSP-MYC-B sequence was first carried out using a human brain cDNA library, and positive clones were further screened by the two-hybrid assays on the c-Myc as a bait. One of the two cDNAs thus cloned encoded the CCAAT box binding protein subunit, CBF-C/NF-YC.In vitro binding assays showed that c-Myc directly bound to CBF-C/NF-YC, and the association between the two proteins was also observed in vivo in cultured cells. In bandshift assays on the wtB probe with the nuclear extract prepared from mouse Balb3T3 cells, two distinct DNA-protein complexes containing c-Myc, the complexe … More s I and II, were observed, and the complex I contained CBF in addition to c-Myc. When the purified CBF subunits were incubated with the wtB probe, the proteins recognized only CCAAT in the wtB sequence even in the presence of purified c-Myc, while the complex I with the nuclear extract required the HSP-MYC-B element as well as CCAAT in the wtB.Similarly, both the HSP-MYC-B element and the CCAAT sequence were required for the transcriptional activity of the wtB sequence. In the transient transfection experiments in mouse Balb3T3 cells, c-Myc first stimulated and then repressed the transcription derived from wtB-hsp70 TATA, according to the doses of c-Myc introduced. The repressed transcription by a high dose of c-Myc was abrogated by the introduction of CBF/NF-Y in a dose-dependent manner. Bandshift assays using the nuclear extract of the cells similarly transfected revealed that the efficiency of the complex I formation w a parallel to the transcriptional activity of wtB.Furthermore, CBF/NF-Y-c-Myc complex possessing the wtB binding activity appeared in the early Gi phase of the cell cycle when the c-Myc expression was not high, and gradually disappeared after the expression level of c-Myc reached maximal. The results indicate that the cell cycle-dependent up- and down- regulation of the hsp70 gene expression is determined by the complex formation states between CBF/NF-Y and c-Myc, controlled by the intracellular amount of c-Myc. Less
我们先前已经在人类HSP70启动子转录起始点-150处发现了HSP-MYC-B元件,该元件可被c-Myc蛋白复合体识别,并参与该基因的早期G1特异性表达。为了鉴定与HSP-MYC-B形成的c-Myc复合体有关的蛋白质并参与G1的特异表达,首先利用人脑cDNA文库在酵母中进行了HSP-MYC-B序列的单杂交筛选,并以c-Myc为诱饵对阳性克隆进行了筛选。其中一个编码CCAAT盒结合蛋白亚基CBF-C/NF-YC。体外结合实验表明,c-Myc直接与CBF-C/NF-YC结合,在培养细胞中也观察到两种蛋白之间的相互作用。在用小鼠Balb3T3细胞核提取液对wtb探针的带移分析中,两种不同的含有c-myc的dna-蛋白质复合体,即复合体…观察到更多的S I和II,复合体I除含有c-Myc外,还含有CBF。当纯化的CBF亚基与WTB探针孵育时,即使在纯化的c-Myc存在的情况下,蛋白质也只识别WTB序列中的CCAAT,而与核提取物的复合体I既需要WTB中的HSP-MYC-B元件,也需要WTB中的CCAAT。同样,WTB序列的转录活性既需要HSP-MYC-B元件,也需要CCAAT序列。在小鼠Balb3T3细胞的瞬时转染实验中,c-Myc先刺激后抑制WTB-HSP70TATA的转录,根据c-Myc的引入剂量。高剂量c-Myc抑制转录可通过CBF/NF-Y以剂量依赖的方式被取消。同样转染组的细胞核提取物的带移分析表明,复合体I的形成效率与wtB的转录活性成正比。具有WTB结合活性的CBF/NF-Y-c-Myc复合体在c-Myc表达水平不高时出现在细胞周期的早期Gi期,并在c-Myc表达达到最高水平后逐渐消失。结果表明,细胞周期依赖性的HSP70基因表达的上调和下调取决于CBF/NF-Y和c-Myc之间的复合体形成状态,并受细胞内c-Myc的含量控制。较少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Mori,K: "MM-1,a novel C-MYC associating protein which represses transcriptional activity of C-MYC" J.Biol.Chem.273. 29794-29800 (1998)
Mori,K:“MM-1,一种新型 C-MYC 相关蛋白,可抑制 C-MYC 的转录活性”J.Biol.Chem.273。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Mori, K., Maeda, Y., Kitaura, H., Taira, T., Iguchi-Ariga, S.M.M.and Ariga, H.: "MM-1, a novel C-MYC associating protein which represses transcriptional activity of C-MYC." J.Biol.Chem.273. 29794-29800 (1998)
Mori, K.、Maeda, Y.、Kitaura, H.、Taira, T.、Iguchi-Ariga, S.M.M. 和 Ariga, H.:“MM-1,一种新型 C-MYC 关联蛋白,可抑制 C- 的转录活性
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Taira,T.: "A novel G1/S-specific enhancer identified in the human heat shock protein 70" Nucleic Acids Res.25. 1975-1983 (1997)
Taira,T.:“在人类热休克蛋白 70 中鉴定出一种新型 G1/S 特异性增强子”Nucleic Acids Res.25。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Tsuchiya,T.: "Ku antigen binds to Alu family DNA" J.Biochem.123. 120-127 (1998)
Tsuchiya,T.:“Ku 抗原与 Alu 家族 DNA 结合”J.Biochem.123。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Nagakubo, D.: "DJ-1, a novel oncogene product which transforms mouse NIH3T3 cells in cooperation with H-ras." Biochem.Biophys.Res.Comm.231. 509-513 (1997)
Nagakubo, D.:“DJ-1,一种新型癌基因产物,可与 H-ras 配合转化小鼠 NIH3T3 细胞。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
TAIRA Takahiro其他文献
TAIRA Takahiro的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('TAIRA Takahiro', 18)}}的其他基金
Effect of respiratory disease onset and functionality in the alveolar-bronchial of oxidative stress defense protein DJ-1
氧化应激防御蛋白DJ-1对呼吸系统疾病发病和肺泡支气管功能的影响
- 批准号:
25461184 - 财政年份:2013
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on transcriptional activation mechanism of DJ-1 gene(PARK7) and the trial to Parkinson's disease cure development
DJ-1基因(PARK7)转录激活机制研究及帕金森病治疗开发尝试
- 批准号:
21590091 - 财政年份:2009
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional analysis of DJ-1, a causative gene product for familial Parkinson's disease
家族性帕金森病致病基因产物 DJ-1 的功能分析
- 批准号:
17590049 - 财政年份:2005
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Function of MM-1, a novel c-Myc-binding protein, as a tumor suppressor
MM-1(一种新型 c-Myc 结合蛋白)作为肿瘤抑制因子的功能
- 批准号:
15590053 - 财政年份:2003
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Function of MM-1, a novel c-Myc-binding protein, as a tumor suppressor
MM-1(一种新型 c-Myc 结合蛋白)作为肿瘤抑制因子的功能
- 批准号:
13672269 - 财政年份:2001
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似国自然基金
TAMs外泌体源LncRNA GHET1通过HSP70调控胃癌细胞EMT促进顺铂耐药的机制研究
- 批准号:JCZRYB202500668
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
热休克蛋白HSP70介导mRNA黏膜疫苗免疫增强作用的机制研究
- 批准号:
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
噪声性聋小鼠内耳组织来源细胞外囊泡
Hsp70对巨噬细胞的调控及机制
- 批准号:
- 批准年份:2025
- 资助金额:10.0 万元
- 项目类别:省市级项目
雌激素受体α介导Hsp70/Casp8泛凋亡复合体调控肾脏缺血再灌注损伤机制研究
- 批准号:
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
银杏双黄酮调控巨噬细胞HSP70蛋白SUMOylation抗非小细胞肺癌EMT的机制研究
- 批准号:MS25H280056
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
肿瘤-视网膜抗原 recoverin 与 HSP70 的融合蛋
白作为乳腺癌肿瘤疫苗的可行性
- 批准号:TGY24H100010
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
基于HSP70调控COX-2泛素化降解探讨益气除痰方抑制肿瘤相关中性粒细胞抗肺癌转移的机制
- 批准号:2024A02042
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
脑靶向外泌体递送HSP70mRNA改善睡眠剥夺小鼠认知功能障碍及机制的研究
- 批准号:2024Y9436
- 批准年份:2024
- 资助金额:15.0 万元
- 项目类别:省市级项目
大鼠骨关节炎软骨细胞凋亡的HSP70-P53 调控机制
- 批准号:2024JJ7517
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
p300介导组蛋白乳酸化促进肿瘤外泌体Hsp70/90诱导的癌症恶病质肌肉萎缩的机制研究
- 批准号:
- 批准年份:2024
- 资助金额:0 万元
- 项目类别:青年科学基金项目
相似海外基金
Hsp40 and Hsp70 in Membrane Protein Triage
膜蛋白分类中的 Hsp40 和 Hsp70
- 批准号:
10718226 - 财政年份:2023
- 资助金额:
$ 1.6万 - 项目类别:
Chaperone protection in Lewy body and Alzheimer’s dementias: determining the structural, molecular and cellular mechanisms of a novel, non-canonical Hsp70 action blocking a-synuclein oligomerization
路易体和阿尔茨海默氏痴呆中的伴侣保护:确定阻断 α-突触核蛋白寡聚化的新型非典型 Hsp70 作用的结构、分子和细胞机制
- 批准号:
10649331 - 财政年份:2023
- 资助金额:
$ 1.6万 - 项目类别:
温罨法が慢性炎症と動脈硬化度に与える影響
热敷对慢性炎症和动脉硬化的影响
- 批准号:
22K17448 - 财政年份:2022
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Investigating the structural mechanism of the hYVH1-Hsp70 complex
研究 hYVH1-Hsp70 复合物的结构机制
- 批准号:
573516-2022 - 财政年份:2022
- 资助金额:
$ 1.6万 - 项目类别:
University Undergraduate Student Research Awards
Understanding the Viral Proteostasis reveals Host Protein Quality Control Mechanisms
了解病毒蛋白质稳态揭示宿主蛋白质质量控制机制
- 批准号:
22H02876 - 财政年份:2022
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Investigating the neuroprotective role of carboxyl-terminus of Hsp70-interacting protein (CHIP) in healthy aging
研究 Hsp70 相互作用蛋白 (CHIP) 羧基末端在健康衰老中的神经保护作用
- 批准号:
557907-2021 - 财政年份:2022
- 资助金额:
$ 1.6万 - 项目类别:
Postgraduate Scholarships - Doctoral
Production of molecular chaperone-enhanced bovine embryos by utilizing heat-independent HSP70 inducer
利用不依赖热的 HSP70 诱导剂生产分子伴侣增强牛胚胎
- 批准号:
22K19233 - 财政年份:2022
- 资助金额:
$ 1.6万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
The Role of HSP70 in Cellular Freeze Tolerance
HSP70 在细胞抗冻性中的作用
- 批准号:
572262-2022 - 财政年份:2022
- 资助金额:
$ 1.6万 - 项目类别:
University Undergraduate Student Research Awards
Dual Role of HSP70 in Diabetes-Induced Vascular Dysfunction
HSP70 在糖尿病引起的血管功能障碍中的双重作用
- 批准号:
10515009 - 财政年份:2022
- 资助金额:
$ 1.6万 - 项目类别:
Exploring the effects of abacavir on HSP70 modulation of alpha-synuclein-mediated neurodegeneration
探索阿巴卡韦对 α-突触核蛋白介导的神经变性的 HSP70 调节的影响
- 批准号:
486151 - 财政年份:2022
- 资助金额:
$ 1.6万 - 项目类别:
Studentship Programs