D-アミノ酸の組織内分布

制备了针对戊二醛偶联物d -天冬氨酸（D-Asp）的特异性多克隆抗体，并通过亲和层析纯化。用抗体对大鼠松果体进行免疫组化染色，发现D-Asp存在于松果体细胞的细胞质中，松果体细胞是松果体的主要细胞类型。妊娠第12天，D-Asp在后脑、中脑和尾侧前脑表现出较弱的免疫反应性（IR），而在妊娠第15 20天，免疫反应性增强并扩展到全脑。在发育早期未成熟的前脑区域（E12）， IR主要是神经元细胞的细胞质特征，而在更成熟的后脑区域，IR定位于轴突带。在发育后期（E18）分化程度更高的前脑中，IR仅限于主要由轴突和突起组成的区域。在睾丸中，生殖细胞的细胞质中有明显的D-Asp IR，特别是在生殖细胞最成熟的细长精细胞丰富的区域周围。在精母细胞和圆形精子细胞质中也可见弱IR。

Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. At gestational day (E) 12, weak immunoreactivity (IR) of D-Asp was apparent at the hindbrain, midbrain and caudal forebrain, whereas it became more intense and extended over the whole brain at 15 20. In the region of the immature forebrain at an early stage of development (E12), IR was mainly a characteristic of the cytoplasm of the neuronal cells, while in the more mature hindbrain it was localized in the axonal zone. In the more differentiated forebrain at a later stage of development (E18), the IR became restricted to zones which mainly consisted of axons and processes. In the testis, D-Asp IR was evident in the cytoplasm of germ cells, especially around the region rich in elongate spermatids, the most mature of the germ cells. Weak IR was also noted in cytoplasm of spermatocytes and round spermatids.