D-アミノ酸の組織内分布

D-氨基酸的组织分布

• 批准号: 09672215
• 负责人: HOMMA Hiroshi
• 金额: $ 1.92万
• 依托单位: THE UNVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672215/
• 关键词: D-Aspartate; immunohistochemistry; pineal gland; adrenal gland; testis; neuroblastic cell; central nervous system; elongate spermatid; D-アミノ酸; アドレナリン; ラット

Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. At gestational day (E) 12, weak immunoreactivity (IR) of D-Asp was apparent at the hindbrain, midbrain and caudal forebrain, whereas it became more intense and extended over the whole brain at 15 20. In the region of the immature forebrain at an early stage of development (E12), IR was mainly a characteristic of the cytoplasm of the neuronal cells, while in the more mature hindbrain it was localized in the axonal zone. In the more differentiated forebrain at a later stage of development (E18), the IR became restricted to zones which mainly consisted of axons and processes. In the testis, D-Asp IR was evident in the cytoplasm of germ cells, especially around the region rich in elongate spermatids, the most mature of the germ cells. Weak IR was also noted in cytoplasm of spermatocytes and round spermatids.

将D-天冬氨酸（D-Asp）与戊二醛偶联，经亲和层析纯化，制备了特异性多克隆抗体。大鼠松果体的免疫组织化学染色的抗体证明了D-Asp的存在下，在松果体细胞的细胞质中，在这个腺体的主要细胞类型。在妊娠第12天（E），弱免疫反应（IR）的D-Asp是明显的后脑，中脑和尾前脑，而它变得更加激烈，并在整个大脑在15 - 20扩展。在该地区的未成熟的前脑在早期的发展阶段（E12），IR主要是一个特征的神经元细胞的细胞质，而在更成熟的后脑，它被定位在轴突区。在分化程度更高的前脑发育后期（E18），IR仅限于主要由轴突和突起组成的区域。在睾丸中，D-Asp IR在生殖细胞的细胞质中是明显的，特别是在最成熟的生殖细胞中的细长精子细胞丰富的区域周围。精母细胞和圆形精子细胞胞质内也有弱的免疫反应。

项目成果

Sakai,K.et al.: "Emergence of D-aspartic acid in the differentiating neurons of the rat central nervous system" Brain Res.808. 65-71 (1998)

Sakai,K.et al.：“D-天冬氨酸在大鼠中枢神经系统分化神经元中的出现”Brain Res.808。

Lee,J-A.et al.: "Immunohistochemical localization of D-aspartate in the rat pineal gland" Biochem.Biophys.Res.Commun.231. 505-508

Lee, J-A. 等人：“D-天冬氨酸在大鼠松果体中的免疫组织化学定位”Biochem.Biophys.Res.Commun.231。

Lee, J-A.et al: "Immunohistochemical localization of D-aspartate in the rat pineal gland" Biochem.Biophys.Res.Commun.231. 505-508 (1997)

Lee, J-A.等人：“D-天冬氨酸在大鼠松果体中的免疫组织化学定位”Biochem.Biophys.Res.Commun.231。

Sakai,K.et al.: "Localization of D-aspartic acid in elongate spermatids in rat testis" Arch.Biochem.Biophys.351. 96-105 (1998)

Sakai，K.等人：“D-天冬氨酸在大鼠睾丸细长精子细胞中的定位”Arch.Biochem.Biophys.351。

Sakai,K.et al.: "D-Aspartic acid localization during postnatal development of rat adrenal gland" Biochem.Biophys.Res.Commun.235. 433-436 (1997)

Sakai,K.et al.：“大鼠肾上腺产后发育过程中的 D-天冬氨酸定位”Biochem.Biophys.Res.Commun.235。