Evaluation of in vivo enzymatic activity of cortisol metabolism associated with hypertension

One of the most unique advatages of the use of isotopically labeled compounds as tracers is that anendogenous compound and its exogenously administered stable isotopically labeled模拟separately measurable by使用mass spectrometry.This study is associated with a new approach usingstable isotope methodology in evaluating 11β-高敏感activities in vivo based on urinary excretion ofunconjugated cortisol and cortisone. The method involved The measurement of stable isotopicallylabeled cortisol with D12 D1H and its labeled metabolitecortisone- D12 cortisone D1H by GC-MS simultaneously with endogenous cortisol and cortisone after oraladministration of a few mg of D12 D1H-labeled cortisol to normal human subjects.This stableisotope approach提供了独特的位置，因为它可以用于measure stable isotopically labeledcortisol and its labeled metabolite cortisone simultaneously with endogenous （unlabeled）cortisone. Very important was the potential use of stable isotopically labeled cortisol in manas a tracer for the native cortisol to cortisone in thekidney. Our results from the stable isotope methodology strongly suggest that the measurement ofunconjugated cortisol and cortisone in urine is a significant advance in assessing renal 11β-HSD2activity in man。

One of the most unique advatages of the use of isotopically labeled compounds as tracers is that an endogenous compound and its exogenously administered stable isotopically labeled analog are separately measurable by using mass spectrometry.This study is associated with a new approach using stable isotope methodology in evaluating 11β-HSD activities in vivo based on urinary excretion of unconjugated cortisol and cortisone. The method involved the measurement of stable isotopically labeled cortisol with ィイD12ィエD1H and its labeled metabolite, cortisone-ィイD12ィエD1H by GC-MS simultaneously with endogenous cortisol and cortisone after oral administration of a few mg of ィイD12ィエD1H-labeled cortisol to normal human subjects.This stable isotope approach offered unique advantages since it allowed to measure stable isotopically labeled cortisol and its labeled metabolite cortisone simultaneously with endogenous (unlabeled) cortisol and cortisone. Very important was the potential use of stable isotopically labeled cortisol in man as a tracer for the native cortisol to recognize the conversion of cortisol to cortisone in the kidney. Our results from the stable isotope methodology strongly suggest that the measurement of unconjugated cortisol and cortisone in urine is a significant advance in assessing renal 11β-HSD2 activity in man.