Analysis of Staphylococcus aureus and Escherichia coli isolates using proton magnetic resonance spectroscopy

使用质子磁共振波谱分析金黄色葡萄球菌和大肠杆菌分离株

• 批准号: 09672363
• 负责人: OHARA Tomoko
• 金额: $ 0.83万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672363/
• 关键词: nuclear magnetic resonance; NMR; Staphylococcus aureus; strain identification; MRSA; 肺炎桿菌; 菌株同定; プロトン核磁気共鳴; メチシリン耐性黄色ブドウ球菌; 院内感染; ヒト病原菌

First we applied proton nuclear magnetic resonance (ィイD11ィエD1H NMR) spectroscopy to distinguish between four bacterial species : Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosae and Kiebsiella pneumoniae. Because of differences in the composition of their cell wall and cytoplasmic constituents, individual bacterial species exhibited characteristic spectra except for K pneumoniae. Reproducibility was satisfactory in the quantitative studies for chemical shift, intensity of signal and integrated intensity. The turnaround time of the test was only about 40 minutes. ィイD11ィエD1H NMR could be a new powerful tool for bacterial identification.Moreover, we analyzed ィイD11ィエD1H NMR spectra for 50 methicillin-resistant Staphylococcus aureus (MRSA) isolates from 42 patients to evaluate the stability and the ィイD11ィエD1H NMR differences among bacterial strains. Each strain had a reproducible, characteristic NMR spectral pattern containing eight to nine specific resonance or groups of re … More sonances in the 0.5 to 4.5 ppm spectral region. Six resonance groups were identified according to the resonance of the highest peak (group A - F). The relative integrated intensity (mean ± SD, normalized to group C=1) of each group was 0.49 ± 0.16 (group A), 3.93 ± 0.81 (group B), 0.38 ± 0.22 (group D), 1.51 ± 0.96 (group E) and 2.18 ± 0.47 (group F). Regarding to reproducibility of the NMR spectra, the C.V. was less than 10% in all groups except group E. The configuration of each NMR spectral group among replicates of the four strains used in the reproducibility was almost identical with only minor variations in the demonstrated intensity of resonances. Storage at 4°C for 4 months or destruction of the microorganisms by ultrasound did not alter ィイD11ィエD1H NMR spectra. NMR spectra of two MRSA strains that had identical DNA patterns by the restriction enzyme were similar. The correlation coefficient between the two NMR spectra was 0.998. Thus, ィイD11ィエD1H NMR spectroscopy appears to be a useful tool for strain identification. Less

首先，我们应用质子核磁共振（NMR）光谱来区分四种细菌：大肠杆菌，金黄色葡萄球菌，铜绿假单胞菌和Kiebsiella肺炎。由于其细胞壁和细胞质成分的组成的差异，个别细菌物种表现出的特征谱，除了肺炎克雷伯氏菌。在化学位移、信号强度和积分强度的定量研究中，重现性令人满意。测试的周转时间只有大约40分钟。为探讨MRSA的稳定性及不同菌株间的NMR谱差异，对42例患者的50株MRSA进行了NMR谱分析。每种菌株都有一个可重复的、特征性的NMR谱图，其中包含8到9个特定的共振或基团。 ...更多信息 0.5至4.5 ppm光谱区域的声音。根据最高峰的共振频率将其分为六个共振组（A ~ F组）.各组的相对积分强度（平均值± SD，标准化为C=1组）分别为0.49 ± 0.16（A组）、3.93 ± 0.81（B组）、0.38 ± 0.22（D组）、1.51 ± 0.96（E组）和2.18 ± 0.47（F组）。关于NMR光谱的再现性，除E组外，所有组的C. V.均小于10%。重现性中使用的四种菌株重复样品中每个NMR光谱组的构型几乎相同，仅在共振强度方面存在微小变化。在4°C下储存4个月或通过超声破坏微生物不会改变β-D11 β-D1 H NMR光谱。两个MRSA菌株的NMR谱具有相同的DNA模式的限制性内切酶是相似的。两个NMR谱之间的相关系数为0.998。因此，核磁共振D11核磁共振D1 H谱似乎是一个有用的工具，应变鉴定。少

项目成果

Ohara T: "Ultrasound instruments as possible vectors of Staphylococcus infection."J Hosp Infec. 40. 73-77 (1998)

Ohara T：“超声仪器可能是葡萄球菌感染的载体。”J Hosp Infec。

Ohara T: "Reevaluation of laboratory parameters in relation to histopathological findings in primary and secondary Sjogren's syndrome."Internal Medicine. (in press).

Ohara T：“重新评估与原发性和继发性干燥综合征组织病理学结果相关的实验室参数。”内科。

Ohara Tomoko: "Reevaluation of laboratory parameters in relation to histological findings in primary and secondary S*ogren's syndrome"Internal Medicine. (in press).

Ohara Tomoko：“重新评估与原发性和继发性 S*ogren 综合征组织学结果相关的实验室参数”内科。

Y.Saito et al: "Epidemiologic Typing of Methicillin-Resistant Staphyloceecn aureus in Neonate Intensive Care Units Using Pulsed-Field Gel Electrophores's" Microbiol.Immunol. 42. 723-729 (1998)

Y.Saito 等人：“使用脉冲场凝胶电泳对新生儿重症监护病房中耐甲氧西林金黄色葡萄球菌进行流行病学分型”Microbiol.Immunol。

Ohara Tomoko: "Reevaluation of laboratory parameters in relation to histological findings in primary and secondary Sjogren's syndrome"Internal Medicine. (in press). (1999)

Ohara Tomoko：“重新评估与原发性和继发性干燥综合征组织学结果相关的实验室参数”内科。