Fate and behavior of microplastics in biosolids: pollution characteristics and environm ental risks

生物固体中微塑料的归宿和行为：污染特征和环境风险

• 批准号: 22KF0162
• 负责人: 李 富生
• 金额: $ 1.41万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KF0162/
• 关键词: Earthworms; microplastics; cow dung; microbes; vermicomposting

Vermireactors were used for treating three different concentrations (0.5%, 1% and 2%) of LDPE and PBSA of <2 mm size with the addition of cow dung (CD). The CD supported the growth and hatchlings of earthworms, and the toxicity effect of both LDPE and PBSA microplastics on earthworm Eisenia fetida was analysed. For vermicomposting, the mixtures were kept for thermo stabilization for at least a week. After that, clitellated Eisenia fetida species of earthworms were incubated separately @ 5 worms’ 250 g-1 of the CD for a period of 120 days. Experimental samples of both CD and earthworms were collected at 60 and 120 days of vermicomposting and analyzed for various physico-chemical parameters, like the moisture content measured by drying the samples to a constant weight at 105 °C (24 h) in an oven. The organic matter content was determined by combusting the dried samples in a muffle furnace at 600 °C for 2 h. The pH and electrical conductivity (EC) were also determined by using the aqueous solution of dry sample with deionized water (w/v = 1/10) after shaken for 2 h. Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR) analysis were also conducted following standard protocols. The initial results suggested that the LDPE microplastics pose more threat to earthworm Eisenia fetida as compared to the PBSA microplastics.

用蠕虫反应器处理3种不同浓度(0.5%、1%和2%)的LDPE和2 mm大小的PBSA并添加牛粪(Cd)。Cd对蚯蚓的生长和孵化有促进作用，并分析了LDPE和PBSA微塑料对蚯蚓的毒性效应。对于蠕虫堆肥，混合物要保持至少一周的热稳定。然后，在5条虫250g~(-1)的Cd浓度下，将带阴蒂的赤子爱胜虫分别孵化120天。在蠕虫堆肥的60天和120天收集Cd和蚯蚓的实验样品，并分析各种物理化学参数，例如在105°C(24小时)的烤箱中将样品烘干到恒重所测得的水分。将干燥样品在马弗炉中600℃下燃烧2 h，测定有机质含量，用去离子水(w/v=1/10)的水溶液摇动2 h，测定样品的pH值和电导率，并进行扫描电子显微镜(SEM)和傅立叶变换红外光谱(FTIR)分析。初步结果表明，与PBSA微塑料相比，LDPE微塑料对蚯蚓的威胁更大。

项目成果

Chapter 14: Removal potential of microplastics in organic solid wastes via biological treatment approaches. In Microbial Biotechnology: Role in Ecological Sustainability and Research

第 14 章：通过生物处理方法去除有机固体废物中微塑料的潜力。

• 发表时间: 2022
• 作者: Bhat;S. A.;Cui;G.;Yaseera;N.;Lei;X.;Ameen;F.;& Li;F.
• 通讯作者: F.