Elucidation of pathological control mechanism of multiple myeloma by tPA/tPA receptor signaling

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This study aimed to find an efficient method to generate tissue-type plasminogen activator (tPA) fragments, which naturally occur during tumor progression/inflammation of the hematologic cancer called multiple myeloma. To determine whether it will occur. In the first year of this proposal, we used different approaches to generate tPA fragments. Using a plasmid-based expression system in bacteria, we evaluated multiple proteases, including alpha elastase and plasmin, to cleave the purchased protein. We have successfully expressed the fragment in bacteria and are currently evaluating various methods for large-scale isolation. In one of his original articles published this year, we identified p53 as a downstream target of the small molecule and plasmin inhibitor YO2 (Cancers 15, 288; doi 10.3390/cancers15010288, 2023). A published review article summarizes the knowledge of the plasminogen-plasmin system in the oral cavity (Cells, 12(3), 445 https://doi.org/10.3390/cells12030445, 2023)."