Structure, function, and dynamics of the TOC core complex (P17)

蛋白质跨膜转运是细胞功能的核心，涉及特定的膜整合蛋白质复合物。外质体膜（TOC）的易位子由至少四个亚基组成-三个受体蛋白Toc 34，Toc 64和Toc 159，以及桶形通道Toc 75。虽然描述了TOC的全局功能，但对分子和原子水平的机制知之甚少。我们将走向整个TOC复合物的结构分析，除了Toc 34和Toc 159的GTdR循环的表征作为易位的中心部分。这些信息将统一与探索这个复杂的动态在不同的发展阶段和组织提供了一个基础的分子理解的易位过程一般。

Protein translocation across membranes is central for cellular function and involves specific membrane integral proteinaceous complexes. The translocon of the outer plastidic membrane (TOC) is composed of at least four subunits - the three receptor proteins Toc34, Toc64 and Toc159, and the barrel shaped channel Toc75. While the global function of the TOC is described, not much is known about the mechanisms at molecular and atomistic levels. We will move toward a structural analysis of the entire TOC complex in addition to the characterization of the GTPase cycle of Toc34 and Toc159 as central part for the translocation. This information will be unified with the exploration of the dynamics of this complex in different developmental stages and tissues to provide a basis for a molecular understanding of the translocation process in general.