Experimental Study of Artificial Tendon Sheath Using Synovial Cell and Collagen Membrane
滑膜细胞和胶原膜人工腱鞘的实验研究
基本信息
- 批准号:09671498
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
When tendons were injured, massive scar fomation around the repaired tendon caused poor clinical results, such as limitation of range of motion, pain, swelling, etc. But there are few literature about the mechanism of scar formation. Scar formation around the sutured tendon and the role of paratenon and epitenon were investigated histologically in this study.Bilateral Achilles! tendons of rabbits were cut and sutured. On the right side, paratenon and epitenon were repaired (repaired group) , and on the left side, they were resected (resected group). Scar formation around the sutured tendon was investigated histologically at 1, 2, 3, 4 weeks after the operation. At I week, fibroblasts and loose connective tissue were recognized in both groups. They were dominant in resected group than in repaired group. At 2 week, vascuralization and cellular infiltration were dominant in resected group than in repaired group. At 3 weeks, the amount of collagen fiber has increased around the sutured tendon much more in resected group than in repaired group. At 4 weeks, there was no remarkable difference with at 3 weeks. Therefore, repairing of paratenon and epitenon is important to reduce scar formation.Our future study project is the development of artificial tendon sheath. We will culture fibroblasts from paratenon and epitenon on collagen membrane, and transport them around the sutured tendon. If this procedure will be established, it will contribute to the clinical result after tendon and ligament surgery.
肌腱损伤后,修复后的肌腱周围形成大量瘢痕,导致肌腱活动受限、疼痛、肿胀等不良临床效果,但关于瘢痕形成机制的文献报道较少。本研究从组织学角度探讨了肌腱缝合后瘢痕的形成以及腱旁和腱外膜的作用。切断兔肌腱并缝合。右侧修复腱旁和腱外膜(修复组),左侧切除腱旁和腱外膜(切除组)。术后1、2、3、4周分别观察肌腱周围瘢痕形成情况。第1周时,两组均可见成纤维细胞和疏松结缔组织。手术切除组较手术修复组优势明显。2周时,切除组血管化和细胞浸润明显多于修复组。术后3周,切除组缝合肌腱周围胶原纤维数量明显增多,修复组明显少于切除组。4周时与3周时无显著差异。因此,修复腱旁和腱外膜对减少瘢痕形成具有重要意义,人工腱鞘的研制是今后的研究方向。将腱旁和腱外膜的成纤维细胞培养在胶原膜上,并将其运输到缝合肌腱周围。如果这种方法能够建立,将有助于肌腱和韧带手术后的临床效果。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Osami Suzuki, Yu Mochizuki, Kenji Kimori, Takasi Shigenobu, Yoshikazu Ikuta: "The Experimental Study of Scar Formation around the Repaired Tendon in Rabbit Model" The Central Japan Journal of Orthopaedic Surgery & Traumatology. Vol.42. 19-20 (1999)
Osami Suzuki、Yu Mochizuki、Kenji Kimori、Takasi Shigenobu、Yoshikazu Ikuta:“兔模型修复肌腱周围疤痕形成的实验研究”日本中部整形外科杂志
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
鈴木 修身 他: "損傷腱修復過程における癒着発生の機序に関する実験的研究" 中部日本整形外科災害外科学会誌. 42巻. 19-20 (1999)
Osumi Suzuki等:“损伤肌腱修复过程中粘连机制的实验研究”日本中部整形外科和灾难外科学会杂志第42卷19-20(1999年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
鈴木修身 他: "損傷腱修復過程における癒着発生の機序に関する実験的研究" 中部日本整形外科災害外科学会誌. 42巻. 19-20 (1999)
Osumi Suzuki等:“损伤肌腱修复过程中粘连机制的实验研究”日本中央整形外科学会杂志第42卷19-20(1999年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MOCHIZUKI Yu其他文献
MOCHIZUKI Yu的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MOCHIZUKI Yu', 18)}}的其他基金
The experimental study for the repair of the osteochondral defect by Mesenchymal stem cells using cell sorter
利用细胞分选仪进行间充质干细胞修复骨软骨缺损的实验研究
- 批准号:
14571380 - 财政年份:2002
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The experimental study of the tendor repair
肌腱修复的实验研究
- 批准号:
11671436 - 财政年份:1999
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Development of tendon/tendon sheath reconstruction: A biomechanical study
肌腱/腱鞘重建的发展:生物力学研究
- 批准号:
16K10876 - 财政年份:2016
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Effect of hyarulonic acid on the tendon/tendon sheath reconstruction. An in vivo study
透明质酸对肌腱/腱鞘重建的影响。
- 批准号:
24592242 - 财政年份:2012
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Effect of hyaluronic acid on the excursion resistance of flexor tendon, tendon sheath reconstruction.
透明质酸对屈肌腱偏移阻力、腱鞘重建的影响。
- 批准号:
13671538 - 财政年份:2001
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Experimental Study on the Application of a Hydroxyapatite Artificial Tendon Sheath to Prevent Adhesions after Tendon Repair
应用羟基磷灰石人工肌腱鞘预防肌腱修复术后粘连的实验研究
- 批准号:
03670701 - 财政年份:1991
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)