Molecular and biophysical approaches to study insulin secretory granules and lipid microdomains in vitro and in living insulinoma cells

体外和活胰岛素瘤细胞中研究胰岛素分泌颗粒和脂质微区的分子和生物物理方法

• 批准号: 81118396
• 负责人: Professor Michele Solimena
• 金额: --
• 依托单位: Paul-Langerhans-Institut Dresden (PLID) des Helmholtz-Zentrums München am Universitätsklinikum Carl Gustav Carus der TU Dresden
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/81118396?language=en
• 关键词: Molecular; biophysical; approaches; study; insulin

ICA512 is a receptor of the insulin secretory granules (SGs) whose cytosolic tail is cleaved upon SG exocytosis. This event triggers a signalling cascade that allows pancreatic ß-cells to adjust their insulin release, SG biogenesis and proliferation to metabolic demand. We plan to combine molecular and biochemical approaches with total internal reflection microscopy and fluorescence correlation spectroscopy to quantitatively visualize the cleavage of single ICA512 molecules in relationship to membrane microdomains. This information may benefit our understanding of diabetes and of the dynamic nature of membrane microdomains in relation to hormone secretion and cell signaling.

ICA512是胰岛素分泌颗粒（SG）的受体，其胞质尾部在SG胞吐时被切割。该事件触发信号级联，其允许胰腺β细胞调节其胰岛素释放、SG生物发生和增殖以满足代谢需求。我们计划将联合收割机分子和生物化学方法与全内反射显微镜和荧光相关光谱学相结合，以定量可视化与膜微区相关的单个ICA512分子的切割。这些信息可能有助于我们理解糖尿病和膜微区与激素分泌和细胞信号传导相关的动态性质。