The effect of anesthetics and anesthetic metbolite on mesangial cells proliferation.

麻醉剂和麻醉代谢物对系膜细胞增殖的影响。

• 批准号: 09671596
• 负责人: SATA Takeyoshi
• 金额: $ 0.77万
• 依托单位: University of Occupational Environmental Health(UOEH)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671596/
• 关键词: mesangial cell; ketamine; propofol; [Protein Kinase A; Protein Kinase; 蛋白燐酸化酵素; 〔^3H〕-thymidine; 尿中代謝産物; メサンギウム細胞; ケタミン; protein kinase A; protein kinase C

We studied the effects of intravenous anesthetics ketamine and propofol on rat mesangial cell (MC) proliferation. Ketamine inhibited [3H]tymidine incorporation into the mesangial cells in a concentration-dependent manner (10 mM*1 mM). But, Propofol had no effects on [3H]tymidine incorporation. Ketamine also attenuated the cell number at clinical concentrations and increased cAMP levels in MC.Protein Kinase A inhibitor recovered the inhibition of ketamine on MC proliferation. Ketamine kept the renal blood flow in Rat in vivo. These results suggested that ketamine have inhibitory effects on the MC proliferation via protein kinase pathway and ketamine may be useful to suppress the progression of chronic renal failure.

本文研究了静脉麻醉药氯胺酮和异丙酚对大鼠系膜细胞增殖的影响。氯胺酮以浓度依赖性方式（10 mM*1 mM）抑制[3H]胸苷掺入肾小球系膜细胞。异丙酚对[3H]胸苷掺入无影响。氯胺酮在临床浓度下也可使MC细胞数减少，cAMP水平升高，蛋白激酶A抑制剂可恢复氯胺酮对MC增殖的抑制作用。氯胺酮对大鼠在体肾血流量有维持作用。提示氯胺酮可通过蛋白激酶途径抑制MC增殖，可能对慢性肾功能衰竭的进展有抑制作用。

项目成果

Minami K., et al.: "Inhibitory effect of anesthetics and ethanol on muscarinic receptors expressed in xenopus oocytes." European Journal of Pharmacology. 339. 237-244 (1997)

Minami K. 等人：“麻醉剂和乙醇对爪蟾卵母细胞中表达的毒蕈碱受体的抑制作用。”

Jimi N et.al: "Inhibitory eddects of the intravenous anesthetic,ketamine,on rat mesangial cell proliferation" Anesthesia and Analgesia. 84. 190-195 (1997)

Jimi N 等人：“静脉麻醉剂氯胺酮对大鼠系膜细胞增殖的抑制作用”麻醉和镇痛。

Minami K.,et al.: "Effects of ethanol and anesthetics on type 1 and 5 metabotropic glutamate receptors expressed in xenopus laevis oocytes"Molecular Pharmacology. 53. 148-156 (1998)

Minami K. 等人：“乙醇和麻醉剂对非洲爪蟾卵母细胞中表达的 1 型和 5 型代谢型谷氨酸受体的影响”分子药理学。

Segawa K et.al: "The inhibitory effect of adrenomedullin on rat mesangial cell mitogenesis." Nephron. 74. 577-579 (1996)

Sekawa K 等人：“肾上腺髓质素对大鼠系膜细胞有丝分裂的抑制作用。”