The effect of anesthetics and anesthetic metbolite on mesangial cells proliferation.

本文研究了静脉麻醉药氯胺酮和异丙酚对大鼠系膜细胞增殖的影响。氯胺酮以浓度依赖性方式（10 mM*1 mM）抑制[3H]胸苷掺入肾小球系膜细胞。异丙酚对[3H]胸苷掺入无影响。氯胺酮在临床浓度下也可使MC细胞数减少，cAMP水平升高，蛋白激酶A抑制剂可恢复氯胺酮对MC增殖的抑制作用。氯胺酮对大鼠在体肾血流量有维持作用。提示氯胺酮可通过蛋白激酶途径抑制MC增殖，可能对慢性肾功能衰竭的进展有抑制作用。

We studied the effects of intravenous anesthetics ketamine and propofol on rat mesangial cell (MC) proliferation. Ketamine inhibited [3H]tymidine incorporation into the mesangial cells in a concentration-dependent manner (10 mM*1 mM). But, Propofol had no effects on [3H]tymidine incorporation. Ketamine also attenuated the cell number at clinical concentrations and increased cAMP levels in MC.Protein Kinase A inhibitor recovered the inhibition of ketamine on MC proliferation. Ketamine kept the renal blood flow in Rat in vivo. These results suggested that ketamine have inhibitory effects on the MC proliferation via protein kinase pathway and ketamine may be useful to suppress the progression of chronic renal failure.