PATHOGENESIS OF ACQUIRED RENAL CYSTIC DISEASE OF THE KIDNEYS WITH TUMOR GENESIS -EXPERIMENTAL STUDY-

获得性肾囊性肾病与肿瘤发生的发病机制-实验研究-

• 批准号: 09671633
• 负责人: FUJITA Kiyoshi
• 金额: $ 1.73万
• 依托单位: KAGAWA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671633/
• 关键词: ACDK; RENAL CELL CARCINOMA; CHRONIC RENAL FAILURE; ラット; ストレプトゾトシン; シュウ酸カルシウム; ACDK; RCC

Haemodialysis improved the prognosis of CRF patients. But acquired cystic disease of kidney (ACDK) occurred in original kidneys in long survival cases, which have higher rates of the incidence of renal cell carcinoma(RCC). Whenever it is thought that mucous membranes of ACDK acquired the ability of growth and become to adenocarcinoma through adenoma, its mechanism is still in the dark. In our study we tried to make Rat-ACDK models and researched the roles of the growth factors on its mechanism. 50 SD rats (6 week) were divided into 4 groups. Group A : Control. Group B : forced to intake OXCa 50g cach orally to make ACDK, Group C : forced to drink streptozotocin(SZ) 0.6g each in water to make RCC, Group D : OXCa 50g and SZ 0.6g were fed. 90 days later we gamed the rats and compared with them using HE stained, immunohistochemical stained (cytokeratin and bimentin) and TUNEL stained tissues. As a result we could made renal cysts on Group B and D.But the cysts didn't have RCC.

血液透析改善了CRF患者的预后。但获得性囊性肾病（ACDK）发生在存活时间较长的原发肾，是肾细胞癌（RCC）发生率较高的病例。以往认为ACDK的粘膜是通过腺瘤获得生长能力而发展为腺癌的，但其发生机制尚不清楚。本研究尝试建立大鼠ACDK模型，探讨生长因子在其发病机制中的作用。6周龄SD大鼠50只，随机分为4组。A组：对照组。B组：C组：灌服链脲佐菌素（SZ）0.6g/只，制备RCC; D组：灌服OXCa 50 g和SZ 0.6g。90天后处死动物，取组织行HE染色、免疫组化染色（细胞角蛋白和bimentin）和TUNEL染色。结果：B、D组均能形成肾囊肿，但囊肿内无肾细胞癌。

项目成果

藤田 潔: "腫瘍発生を伴う多嚢化萎縮腎モデルの作成および増殖因子の作用機序に関する研究"新薬と臨症. 50. 267-271 (2001)

Kiyoshi Fujita：“伴随肿瘤发展的多囊性萎缩肾模型的创建和生长因子作用机制的研究”《新药与临床状况》50. 267-271 (2001)。