Molecular biological analysis of head and neck cancer

头颈部癌与环境因素有关，包括烟酒摄入和病毒感染。通过分子生物学分析探讨头颈部肿瘤的生物学行为.人乳头瘤病毒（HPV），尤其是HPV-16或18与扁桃体癌或口腔癌的发生有关.我们研究了细胞周期相关因子cyclin D1和p27（CDK抑制剂家族）是否与独立的预后因素相关。舌癌和下咽癌中cyclin D1高表达或p27低表达与预后不良有关.我们研究了p53抑癌基因突变与头颈部癌发生的关系。p53突变发生率高，突变患者预后差.血管内皮细胞生长因子高表达的患者与淋巴结转移和淋巴结转移不良有关.葡萄糖转运蛋白基因（Glut）1在下咽癌中的表达率为83%，且与Ki-67或p53的过度表达有关。Glut 3的表达率为29%。Glut 1和Glut 3的表达与临床因素无关。

Head and neck carcinoma is related with environmental factors including alcohol or tobacco intake and viral infection. We investigated biologic behavior of head and neck carcinoma by molecular biological analysis.1. Human papilloma virus (HPV), especially HPV-16 or 18 associated with carcinogenesis of tonsillar or oral carcima.2. We examined whether the cell cycle related factors of cyclin D1 and p27 which is a family of CDK inhibitors were associated with independent prognostic factor. High cyclin D1 expression or low p27 expression is related with poor prognosis in the tongue and the hypopharyngeal carcinomas.3. We examined the association between the mutation of p53 tumorsuppressor gene and the development of head and neck carcinomas. p53 mutation occurred high frequently and the patients with p53 mutation showed poor prognosis.4. The patients with high vascular endothelial cell growth factor expression associated with lymph node spread and poor prognosis.5. Glucose transporter gene (Glut) 1 expressed in 83% in hypopharyngeal carcinoma and associated with Ki-67 or p53 overexpressions. Glut3 expressed in 29%. Neither Glut1 nor Glut3 expressions associated with any clinical factors.