Development of the Fetal Endoscopic Surgery

对6只胎羊进行宫内内窥镜气管结扎术，胎龄90~145天(足145天)。使用二氧化碳充气和轻轻握住胎头使胎儿可以在胎儿镜下进行气管结扎，然而，胎儿在48-72小时后会导致死产。通过气管结扎，胎肺的重量几乎扩大了一倍。最终的气管撕裂导致胎肺早产。超声引导下气管穿刺法阻断气管的创伤性较小，存活时间最长可达144小时。Nitrofen诱导的SD大鼠胚胎第20天时，角质形成细胞生长因子(KGF)的mRNA表达减弱，而SP-C和KGFR的表达水平相同。该数据是通过RT-PCR获得的，但结果不具有重复性。北方斑点分析的进一步调查现在正在进行中。

Endoscopic tracheal ligation in utero was attempted in 6 fetal goat at various gestational ages from 90 to 145 days (term-145days). Using CO2 insufflation and gentle holding of the fetal head made fetoscopic tracheal ligation possible, however, fetuses resulted in still birth after 48-72 hours. The fetal lung was expanded almost twice in weight by tracheal ligation. Eventual tracheal laceration caused prematurity of the fetal lung. USG guided tracheal puncture to obstruct trachea seems to be less invasive because the longest survival up to 144 hours was obtained.Nitrofen induced CDH (congenital diaphragmatic hernia) revealed impaired expression of mRNA for Keratinocyte Growth Factor at day 20 embryo of SD rats while SP-C and KGFR were the same level. The data was obtained by RT-PCR, however, the result was not reproducible. Further investigation by the northern blot analysis is now on the way.