Development of the Fetal Endoscopic Surgery

胎儿内窥镜手术的发展

• 批准号: 09671838
• 负责人: MORIKAWA Yasuhide
• 金额: $ 0.7万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671838/
• 关键词: ENDOSCOPIC SURGERY; FETOSCOPY; TRACHEA; FETAL SURGERY; DIAPHRAGMATIC HERNIA; KERATINOCYTE GROWTH FACTOR; SURFACTANT PROTEIN; HYPOPLASTIC LUNG; 内視鏡下手術; 先天性横隔膜ヘルニア; KGF-r; nitrofen

Endoscopic tracheal ligation in utero was attempted in 6 fetal goat at various gestational ages from 90 to 145 days (term-145days). Using CO2 insufflation and gentle holding of the fetal head made fetoscopic tracheal ligation possible, however, fetuses resulted in still birth after 48-72 hours. The fetal lung was expanded almost twice in weight by tracheal ligation. Eventual tracheal laceration caused prematurity of the fetal lung. USG guided tracheal puncture to obstruct trachea seems to be less invasive because the longest survival up to 144 hours was obtained.Nitrofen induced CDH (congenital diaphragmatic hernia) revealed impaired expression of mRNA for Keratinocyte Growth Factor at day 20 embryo of SD rats while SP-C and KGFR were the same level. The data was obtained by RT-PCR, however, the result was not reproducible. Further investigation by the northern blot analysis is now on the way.

对6只胎龄为90 ~ 145天（足月~ 145天）的山羊胎儿进行了子宫内气管结扎术。使用CO2吹入和轻轻握住胎头使胎镜气管结扎成为可能，然而，胎仔在48-72小时后导致死产。通过气管结扎使胎肺扩张近两倍重量。最终气管撕裂导致胎肺早产。结论：超声引导下气管穿刺阻塞气管，可使大鼠存活时间最长达144 h，创伤小，在SD大鼠胚胎20 d时，Nitrofen诱导的先天性腹股沟疝（CDH）可使角质细胞生长因子（Keratinocytegrowthfactor，KGFR）mRNA表达降低，而SP-C和KGFR表达水平相同。通过RT-PCR获得数据，然而，结果不具有可重复性。进一步的研究，北方印迹分析，现在的方式。

项目成果

松村喜一郎、森川康英ほか: "ラット横隔膜ヘルニアの胎仔肺におけるFGF-7並びにレセプターの発現"日小外会誌. 35(3). 631 (1999)

Kiichiro Matsumura、Yasuhide Morikawa 等：“大鼠膈疝胎儿肺中的 FGF-7 和受体的表达”日本外国语学会杂志 35（3）631（1999）。

Morikawa Y,et al: "The impact and role of endosurgery in fotuses and neonates"Proceedings:The tenth Fukuoka International Symposium on Perinatal Medicine. 31-34 (1999)

Morikawa Y等人：“内外科手术对胎儿和新生儿的影响和作用”论文集：第十届福冈国际围产期医学研讨会。