Development of the Fetal Endoscopic Surgery

本文对6只不同胎龄（90 ~ 145天）的胎羊进行了子宫内气管内窥镜结扎术。使用二氧化碳充气和轻柔地握住胎儿头部使胎儿镜气管结扎成为可能，然而，胎儿在48-72小时后导致死产。通过气管结扎，胎儿肺的重量扩大了近两倍。最终气管撕裂导致胎儿肺过早发育。USG引导下气管穿刺阻塞气管似乎侵入性较小，因为最长生存时间达144小时。硝芬诱导的先天性膈疝在SD大鼠胚胎第20天出现角化细胞生长因子mRNA表达受损，而SP-C和KGFR水平相同。数据通过RT-PCR获得，但结果不可重复。目前正在进行北方斑点分析的进一步调查。

Endoscopic tracheal ligation in utero was attempted in 6 fetal goat at various gestational ages from 90 to 145 days (term-145days). Using CO2 insufflation and gentle holding of the fetal head made fetoscopic tracheal ligation possible, however, fetuses resulted in still birth after 48-72 hours. The fetal lung was expanded almost twice in weight by tracheal ligation. Eventual tracheal laceration caused prematurity of the fetal lung. USG guided tracheal puncture to obstruct trachea seems to be less invasive because the longest survival up to 144 hours was obtained.Nitrofen induced CDH (congenital diaphragmatic hernia) revealed impaired expression of mRNA for Keratinocyte Growth Factor at day 20 embryo of SD rats while SP-C and KGFR were the same level. The data was obtained by RT-PCR, however, the result was not reproducible. Further investigation by the northern blot analysis is now on the way.