MOLECULAR MECHANISM OF THE TRACHEAL LIGATION FOR HYPOPLASTIC FETAL LUNG

发育不全胎肺气管结扎的分子机制

• 批准号: 12671743
• 负责人: MORIKAWA Yasuhide
• 金额: $ 1.02万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671743/
• 关键词: KGF; FGF-10; Bmp4; congenital diaphragmatic hernia; tracheal ligation; growth factor; fetal surgery; USG; 横隔膜ヘルニア; 気管結紮; nitrofen; KGF-R

1. Development of the fetal tracheal ligation by minimally invasive techniqueFetal tracheal ligation is considered to be a modality to promote lung development of the hypoplastic lung of congenital diaphragmatic hernia, however, even fetoscopic approach could not be safe to the fetus. The present investigation developed single port approach to occlude the fetal trachea with the sophisticated guidance by USG. A 5 mm port was introduced directly into the oral cavity of the fetal goat and the tracheal intubation was accomplished by USG guidance. A fine detachable micro-balloon was introduced and blown up to occlude the trachea. Fetuses were kept up to five days in uterus, however, all were lost by miscarriage due to intra-uterine fetal death. The present technique seems to be feasible for fetal treatment, although more meticulous care to handle fetuses and pregnant uterus should be reconsidered.1. Change of expression of KGF and Bmp4.Based on our previous study KGF may be less expressed in Nitrofen induced hypoplastic fetal lung. In situ hybridization revealed KGF mRNA expression in the lunf of E14 fetal rat treated by Nitrofen. So far, distinct difference of KGF expression has not been observed in terms of fetal age. Bmp4 is expressed in E12 in both group and once becomes low in E16 of Nitrofen animal and E18 of control fetus. The result may suggest that a possible role of Bmp4 in the late gestational age in the formation of lung hypoplasia induced by Nitrofen.

1.微创技术胎儿气管结扎术的发展 胎儿气管结扎术被认为是促进先天性膈疝发育不全肺发育的一种方式，但即使是胎儿镜手术也对胎儿并不安全。目前的研究开发了单孔方法，在 USG 的复杂引导下封堵胎儿气管。将5mm端口直接引入胎山羊口腔，并在USG引导下完成气管插管。引入一个细小的可拆卸的微气球并使其膨胀以堵塞气管。胎儿在​​子宫内保留长达五天，但由于宫内胎儿死亡而全部因流产而死亡。目前的技术对于胎儿治疗似乎是可行的，尽管应重新考虑对胎儿和怀孕子宫进行更细致的护理。1。 KGF和Bmp4表达的变化。根据我们前期的研究，KGF可能在硝基芬诱导的胎肺发育不全中表达较少。原位杂交显示经硝基芬处理的E14胎鼠肺脏中KGF mRNA的表达。迄今为止，尚未观察到KGF表达在胎龄方面存在明显差异。 Bmp4在两组的E12中都有表达，并且在硝基芬动物的E16和对照胎儿的E18中一度降低。该结果可能表明，Bmp4 在孕晚期硝基芬诱导的肺发育不全的形成中可能发挥作用。