Neuroblastoma and Active Oxygen ; Role in Tumor Outgrowth and Malignancy

神经母细胞瘤和活性氧；

• 批准号: 09671836
• 负责人: SATOMI Akira
• 金额: $ 2.05万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671836/
• 关键词: Neuroblastoma; C 1300 murine; Active Oxygen; Tumor outgrowth; Malignancy

The spontaneous regression of neuroblastorna (NB) , the most common solid malignant tumor in chilhood, is occasionally encountered in younger infants. Host immunity is considered to be related to this phenomenon. The present study was conducted to examine the relationship between active oxygen and the tumor outgrowth in tumor bearing hosts using transplantable C1300 murine NB cell line. The cells (lx 105) were inoculated into newborn A/J mice (within 2 weeks after birth ; group 1) and into adult mice (8 weeks old ; group 2). The animals were sacrificed 1,2,3 and 4 weeks after tumor cell inoculation. The electron spin resonance (ESR) spectra, tissue superoxide dismutase (SOD) activity, lipid hydropeoxide (LPO) and NO (N0_2/N0_3) were measured in the tumor. The cell cycle were also measured, and the results analyzed in relation to SOD activity. The signal of ESR spectra was higher at 2 weeks after inoculation than at the other weeks. SOD activity in the' group 1 was lower than in the group 2, and there were no significant differences week by week. SOD activity in group 2 increased at 1 - 2 weeks, and decreased at 3 weeks. In group 1, LPO didnot varied among weeks, and NO increased at 3 - 4 weeks. In group 2, LPO increased at 3 weeks, and NO was highest at 1 week and decrased at 2 week. These results suggest that there is the difference in the function of NO between group 1 and group 2.

神经母细胞瘤（NB）是儿童期最常见的实体性恶性肿瘤，其自发性消退偶尔发生在年幼的婴儿中。宿主免疫被认为与这种现象有关。本研究采用小鼠NB细胞系C1300，探讨活性氧与肿瘤生长的关系。将细胞（lx 105）接种到新生A/J小鼠（出生后2周内;组1）和成年小鼠（8周龄;组2）中。分别于接种后1、2、3、4周处死动物。测定肿瘤组织超氧化物歧化酶（SOD）活性、脂质过氧化物（LPO）和NO（NO_2/NO_3）。测定细胞周期，并与SOD活性进行相关性分析。接种后第2周ESR谱信号较其他时间高。超氧化物歧化酶活性第1组低于第2组，但各周间无显著性差异。第2组SOD活性在1 ~ 2周升高，3周下降。第1组LPO无明显周间变化，NO在3 ~ 4周升高。第2组3周时LPO升高，NO在第1周时最高，第2周时下降。结果提示，NO在第1组和第2组中的作用存在差异。

项目成果

Masaru Sonoda: "An Assay Method for Nitric Oxide-Related Compounds in whale Blood." ANALYTICAL BIOCHEMISTRY. 247. 417-427 (1997)

Masaru Sonoda：“鲸鱼血液中一氧化氮相关化合物的测定方法。”

Sonoda M,Kobayashi J,Satomi A.et al: "An Assay Method for Nitric Oxide-Related Compounds in Whole Blood" Analytical Biochemistry. 247. 417-427 (1997)

Sonoda M、Kobayashi J、Satomi A.等人：“全血中一氧化氮相关化合物的测定方法”分析生物化学。

里見 昭: "消化器疾患と活性酸素" 日本醫事新報. 3807. 37-42 (1997)

Akira Satomi：“胃肠道疾病和活性氧”日本医学报纸 3807. 37-42 (1997)。

里見 昭: "消火器疾患と活性酸素" 日本監事新報. 3807. 37-42 (1997)

Akira Satomi：“灭火器疾病和活性氧”，日本汉字新报 3807. 37-42 (1997)。