Phytopreventive and Phytotherapeutic Application to Dentistry - Anti-dental caries and anti-periodontal disease activity, and oral retentivity after application of edible phytochemical flavonoids and alkaloids -

为了将植物预防和治疗策略应用于牙科，本论文对食用植物中的黄酮类和生物碱类成分进行了防龋性和抗牙周病活性研究，并对其在口腔中的固位特性和药理机制进行了研究。黄酮类化合物：(1)各种植物的筛选提供了一种对龋齿和MRSA感染具有强大效用的抗菌黄酮类化合物。(2)建立了同时测定唾液中8种儿茶素的高效液相色谱方法。(3)将该方法应用于药理活性多羟基黄酮类化合物的定量研究。(4)口腔药代动力学研究表明，防龋儿茶素在含口后仍保留在口腔内，这种滞留是由于它们在口腔不同部位的吸附所致。(5)以洗必泰为参照物，对比评价儿茶素的防龋性。(6)U-…具有降低膜流动性的作用更重要的是黄酮类化合物的防龋性和抗菌活性。(7)在可食用植物的筛选中，发现洋葱中含有一种类黄酮型胶原酶抑制剂，随后开展了开发抗牙周病植物药物的研究。生物碱：(1)β-卡波林能抑制引起龋齿和牙周病的口腔细菌的生长。(2)。建立了高效液相色谱法测定食用和药用植物中具有药理活性的β-Carolines的方法。(3)在功能性食品的筛选中，酱油中的两种β-Caroline可抑制人的血小板聚集，进而揭示其抗血小板的作用机制。(4)一系列植物化学研究表明，酒精中毒相关的β-Caroline与全身麻醉药/局麻药之间存在相互作用，饮食中β-Caroline代谢缺陷在某种精神障碍的发病机制中起着重要作用。β-Caroline生物碱已被研究为慢性酒精摄入和婴儿自闭症引起麻醉耐受的可能病因。较少

To apply the phytopreventive and phytotherapeutic strategies to dentistry, the flavonoidal and alkaloidal components in edible plants were studied concerning the anti-dental caries and anti-periodontal disease activity, the retentive property in oral cavity, and the pharmacological mechanism.1. Flavonoids : (1) Screenings of various plants provided an antibacterial flavone with the potent utility for dental caries and MRSA infection. (2) An HPLC method was developed for the simultaneous determination of eight catechins in saliva. (3) The method was applied to quantitative studies of pharmacologically active polyhydroxyflavonoids. (4) Oral pharmacokinetic studies revealed that anti-caries catechins were retained in oral cavity after mouthrinsing, and such retentivity was due to their adsorption to different oral sites. (5) The caries-preventive utility of catechins was evaluated comparatively with chlorhexidine as a reference agent. (6) Membrane fluidity-reducing effects were found to u … More nderlie the anti-caries and anti-bacterial actions of flavonoids. (7) In screening of edible plants, onion was found to contain a flavonoidal collagenase-inhibitor , followed by studies to develop the phytomedicine against periodontal diseases.2. Alkaloids : (1) Beta-carbolines inhibited the growth of oral bacteria responsible for dental caries and periodontal diseases. (2). An HPLC method was developed for the determination of pharmacologically active β-carbolines in edible and medicinal plants. (3) In screenings of functional foodstuffs, two β-carbolines in soy sauce inhibited human platelet aggregation, followed by educidation of their anti-platelet mechanism. (4) A series of phytochemical studies suggested the interaction between alcoholism-relating β-carboline and general/local anesthetics, and the responsibility of metabolic deficiency of dietary β-carboline for the pathogenesis of a certain mental disorder. Beta-carboline alkaloids have been studied as a possible etiological factor of anesthetic tolerance by chronic alcohol intake and infantile autism. Less