Phytopreventive and Phytotherapeutic Application to Dentistry - Anti-dental caries and anti-periodontal disease activity, and oral retentivity after application of edible phytochemical flavonoids and alkaloids -

牙科中的植物预防和植物治疗应用 - 抗龋齿和抗牙周病活性，以及​​应用食用植物化学黄酮类和生物碱后的口腔保持力 -

• 批准号: 09671911
• 负责人: TSUCHIYA Hironori
• 金额: $ 0.7万
• 依托单位: Asahi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671911/
• 关键词: Phytoprevention and phytotherapy; Anti-dental caries; Anti-periodontal disease; Flavonoids; Alkaloids; Antibacterial activity; Oral retentivity; Pharmacological mechanism; 酵素阻害; 機能性食品; 抗菌周病; 機能性食物

To apply the phytopreventive and phytotherapeutic strategies to dentistry, the flavonoidal and alkaloidal components in edible plants were studied concerning the anti-dental caries and anti-periodontal disease activity, the retentive property in oral cavity, and the pharmacological mechanism.1. Flavonoids : (1) Screenings of various plants provided an antibacterial flavone with the potent utility for dental caries and MRSA infection. (2) An HPLC method was developed for the simultaneous determination of eight catechins in saliva. (3) The method was applied to quantitative studies of pharmacologically active polyhydroxyflavonoids. (4) Oral pharmacokinetic studies revealed that anti-caries catechins were retained in oral cavity after mouthrinsing, and such retentivity was due to their adsorption to different oral sites. (5) The caries-preventive utility of catechins was evaluated comparatively with chlorhexidine as a reference agent. (6) Membrane fluidity-reducing effects were found to u … More nderlie the anti-caries and anti-bacterial actions of flavonoids. (7) In screening of edible plants, onion was found to contain a flavonoidal collagenase-inhibitor , followed by studies to develop the phytomedicine against periodontal diseases.2. Alkaloids : (1) Beta-carbolines inhibited the growth of oral bacteria responsible for dental caries and periodontal diseases. (2). An HPLC method was developed for the determination of pharmacologically active β-carbolines in edible and medicinal plants. (3) In screenings of functional foodstuffs, two β-carbolines in soy sauce inhibited human platelet aggregation, followed by educidation of their anti-platelet mechanism. (4) A series of phytochemical studies suggested the interaction between alcoholism-relating β-carboline and general/local anesthetics, and the responsibility of metabolic deficiency of dietary β-carboline for the pathogenesis of a certain mental disorder. Beta-carboline alkaloids have been studied as a possible etiological factor of anesthetic tolerance by chronic alcohol intake and infantile autism. Less

为了将植物预防和植物治疗策略应用于牙科，对食用植物中的黄酮和生物碱成分的抗龋齿和抗牙周病活性、口腔滞留特性及药理机制进行了研究。 1．类黄酮：（1）通过对多种植物的筛选，提供了一种对龋齿和MRSA感染具有有效功效的抗菌黄酮。 (2)建立了同时测定唾液中8种儿茶素的HPLC方法。 (3)该方法应用于药理活性多羟基黄酮的定量研究。 (4)口腔药代动力学研究表明，漱口后抗龋齿儿茶素类物质在口腔中保留，这种保留作用是由于其吸附在口腔不同部位的缘故。 (5)以洗必太为参照剂，评价儿茶素类的防龋效果。 (6) 发现膜流动性降低作用增强了类黄酮的抗龋齿和抗菌作用。 (7)在食用植物筛选中发现洋葱中含有黄酮类胶原酶抑制剂，并进行研究开发抗牙周病的植物药。 2．生物碱： (1) β-咔啉可抑制导致龋齿和牙周病的口腔细菌的生长。 （2）。建立了测定食用和药用植物中具有药理活性的β-咔啉的HPLC方法。 (3)在功能食品筛选中，酱油中的两种β-咔啉可抑制人血小板聚集，并阐明其抗血小板作用机制。 (4) 一系列植物化学研究表明，与酗酒相关的β-咔啉和全身/局部麻醉药之间存在相互作用，以及饮食中β-咔啉的代谢缺乏与某种精神障碍的发病机制有关。 β-咔啉生物碱已被研究为长期饮酒和婴儿自闭症引起的麻醉耐受性的可能病因。较少的

项目成果

Hironori Tsuchiya: "Quantitative analysis of all types of β-carboline alkaloids in medicinal plants and dried edible plants by high-performance liquid chromato-graphy with selective fluorometric detection" Phytochemical Analysis. (印刷中).

Hironori Tsuchiya：“通过高效液相色谱法和选择性荧光检测对药用植物和干燥食用植物中所有类型的 β-咔啉生物碱进行定量分析”植物化学分析（正在出版）。

Hironori Tsuchiya et al.: "Nanoscale analysis of pharmacologically active catechins in body fluids by HPLC using before complex extraction pretreatment"Talanta. 46. 717-726 (1998)

Hironori Tsuchiya 等人：“在复杂提取预处理之前，通过 HPLC 对体液中药理活性儿茶素进行纳米级分析”Talanta。

Hironori Tsuchiya: "Bata-carboline alkaloids in crude drugs" Chemical & Pharmaceutical Bulletin. (印刷中).

Hironori Tsuchiya：“生药中的 Bata-咔啉生物碱”化学与制药通报（正在出版）。

Hironori Tsuchiya, Takashi Miyazaki et al: "High-performance liquid chromatographic analysis of chlorhexidine in saliva after mouthrinsing"Caries Research. 33. 156-163 (1999)

Hironori Tsuchiya、Takashi Miyazaki 等人：“漱口后唾液中氯己定的高效液相色谱分析”龋齿研究。

Masaru Sato: "Phytochemical Flavones with the Antibacterial Activity against Methicillin-Resistant Staphylococcus aureus" Dentistry in Japan. (発表予定).

Masaru Sato：“植物化学黄酮对耐甲氧西林金黄色葡萄球菌具有抗菌活性”，日本牙科（待发表）。