Immunohistochemical investigation of Gallyas-postitive glial structures in atypical dementia

非典型痴呆中 Gallyas 阳性神经胶质结构的免疫组织化学研究

• 批准号: 09670983
• 负责人: TAKEUTCHI Tooru
• 金额: $ 0.45万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670983/
• 关键词: argyrophilic structures in glia; Thorn-shaped astrocyte; Tuft-shaped astrocyte; Astrocytic plaque; Glial coiled body; Argyrophilic thread; Argyrophilic end-feet; Thom-shaped astrocyte; Thorn-Shaped astrocyte

We investigated Gallyas-positive glial structures immunohistochemically in cerebral cortex of patients with diffuse neurofibrillaly tangles with calcification (DNTC). DNTC is characterized by slowly progressive cortical dementia in the proscenium, localized temporal or temporofrontal lobar atrophy, numerous neurofibrillary tangles widespread in the cerebral cortex, and pronounced calcareous deposits. In addition to neurofibrillary tangles, a variety tau-positive glial structures occur. Double immunostaining demonstrated that thorn-shaped astrocyte are located in cerebral white matter with severe gliosis and that agglomerate and amorphous argyrophilic structures of partially tau-positive fibers are found in fronto-temporo-parietal cotices and subcortical white matter. Glial coiled body-like structures in cerebral white matter are not stained by teansferrin. Totally tau-positive argyrophilic structures are found numerously in front-temporo parietal cerebral cortex and white matter. Partially tau-positive argyrophilic structures stained by GFAP are recognized in fronto-temporo-parietal cotices. Argyrophilic end-feet are found in regions with severe gliosis. These results suggest that formation and destribution of Gallyas/tau-positive glia in this disorder are different form other types of degenerative disorder with dementia.

我们用免疫组织化学方法研究了弥漫性神经纤维缠结伴钙化（DNTC）患者大脑皮层的galyas阳性胶质细胞结构。DNTC的特征是前庭缓慢进展的皮质痴呆，局部颞叶或颞额叶萎缩，大脑皮层广泛存在大量神经原纤维缠结，以及明显的钙质沉积。除了神经原纤维缠结外，还会出现多种tau阳性胶质结构。双免疫染色显示，严重胶质瘤的大脑白质中存在刺状星形胶质细胞，额颞顶叶皮层和皮层下白质中存在部分tau阳性纤维的聚集性和无定形亲银结构。脑白质中神经胶质卷曲体样结构未被tetransferin染色。在大脑前部颞顶叶皮层和白质中发现大量完全tau阳性的亲银结构。GFAP染色的部分tau阳性亲银结构在额-颞-顶叶皮层被识别。嗜银端足见于严重神经胶质瘤的区域。这些结果表明，该疾病中galyas /tau阳性胶质细胞的形成和分布与其他类型的退行性痴呆疾病不同。