Electrophysiological study of serotonergic function in the CNS

中枢神经系统血清素能功能的电生理学研究

• 批准号: 09671004
• 负责人: TAJIMA Osamu
• 金额: $ 1.86万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671004/
• 关键词: serotonin; 5- HTIA receptor; hippocampus; CA1; 8- OH DPAT; flesinoxan; electrophysiology; rat; 5-HT1Areceptor; serotonin(5-HT); hippocampal neuron; autapse; 8-hydroxy-dipropylamino-tetralin(8-OH-DPAT); whole-cell patch-clamp recording; excit

Serotonin (5-hydroxtyptmine, 5-HT) mediates a wide range of physiological functions by activating multiple receptors. Most 5-HT receptors regulate intracellular signal transduction system through GTP-binding proteins. These intracellular signal transductions mediate by 5-HT receptors of somato-dendritic region of neurons were mostly studied using brain slice preparations. Though 5-HT receptors distribute not only at the somato-dendritic region but also at the postsynaptic region, physiological properties of the postsynaptic 5-HT receptors remain nuclear. We attempted to analyze the action of 5-HT on synaptic transmission using autapse of the rat hippocampal neuron in culture, as both pre- and postsynaptic action could be analyzed in a single cell. The action of 8-OH-DPAT, felsinoxan and fluvoxamine on the epsp induced in the CA1 hippocampal region was also studied using paired-pulse facilitation method. We found that 5-HT suppressed the amplitude of excitatory postsynaptic current and this action was suggested to be presynaptic action and mediated through 5-HT1A receptor.

5-羟色胺（5-hydroxytyptmine，5-HT）通过激活多种受体介导广泛的生理功能。大多数5-HT受体通过GTP结合蛋白调节细胞内信号转导系统。这些细胞内信号转导主要是通过脑片研究的，它们是由神经元体-树突区5-HT受体介导的。虽然5-HT受体不仅分布在体-树区域，而且也分布在突触后区域，但突触后5-HT受体的生理特性仍然是核。我们试图用培养的大鼠海马神经元的autapse分析5-HT对突触传递的作用，因为突触前和突触后作用都可以在单个细胞中分析。采用双脉冲易化法观察8-OH-DPAT、非西诺生和氟伏沙明对海马CA 1区诱发epsp的作用。我们发现5-HT抑制兴奋性突触后电流的幅度，这种作用可能是突触前作用，并通过5-HT 1A受体介导。

项目成果

Kogure,M,Tajima,O,Yamaguchi,K: "Slow synaptic response and modulation"Edited by k.Kuba、H.Higashida,D.A.Brown,T.Yoshioka (Springer-Verlag). 455 (1999)

Kogure，M，Tajima，O，Yamaguchi，K：“缓慢的突触反应和调制”k. Kuba，H. Higashida，D. A. Brown，T. Yoshioka 编辑（Springer-Verlag）455（1999）。

Kogure, M, Tajima, O, Yamaguchi, K.: "Slow synaptic response and modulation"Edited by K. Kuba, H. Higashida, D.A. Brown, T. Yoshioka (Springer- verlag). 226-227 (1999)

Kogure, M、Tajima, O、Yamaguchi, K.：“缓慢的突触反应和调制”编辑：K. Kuba、H. Higashida、D.A.

Kogure, M, Tajima O, Yamaguchi K,: "Slow synaptic response and modulation"Edited by K. Kuba, H. Higashida, D.A.Brown, T. Yoshioka(Springer-Verlag). 455 (1999)

Kogure，M，Tajima O，Yamaguchi K，：“缓慢的突触反应和调制”由 K. Kuba，H. Higashida，D.A. Brown，T. Yoshioka 编辑（Springer-Verlag）。