Effect of fish oil-based fat emulsion on MOF

鱼油基脂肪乳对 MOF 的影响

• 批准号: 09671211
• 负责人: TASHIRO Tsuguhiko
• 金额: $ 1.98万
• 依托单位: Chiba University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671211/
• 关键词: EPA; DHA; Stress response; MOF; Fat emulsion; Immunosuppression; Thermal injury; Cytokine; 免疫抑制剤; 魚油静注用脂肪乳剤; サイトカイン; 腸管translocation

The effects of n-6 and n-3 PUFA on protein metabolism, cell-mediated immunity, and production of cytokines and prostanoids were studied.Experiment 1 : Three groups of rats were fed by TPN including safflower oil emulsion (20% of total energy ; group"N6"), 19% of safflower oil and 1% of structured EPA and DHA (2 : 1)("N3") and without fat ("Control"), and were subjected to 20% full-thickness scald burns. "N6"demonstrated increased serum IL-6 and TNFa (p<0.05), and decreased nitrogen balance (NB), when compared with "Control". But in "N3", TNF and IL-10 were reduced, and NB was improved. Suppressed delayed type hypersensitivity (DTH) induced by burn injury, was significantly improved in "N3", when compared with "Control" and "N6".Experiment 2 : Forty-three rats were fed by TPN including safflower oil (%)/fish oil (%) fat emulsion (0/0, 0/20, 4116, 10/10 and 20/0). DTH, GM-CSF and EPA increased, and gut endotoxin translocation decreased proportionately with the intravenous dose of fish oi … More l emulsion. GM-CSF correlated significantly with DTH.Experiment 3 : Six esophagectomized patients received fat-free TPN ("E-gl"), and 6 received TPN with Intralipid (20% of total energy, "E-fat"). EPA methyl ester (1.8g/day) were administered orally or enterally to 7 who received TPN with Intralipid ("E-EPA"). In group "E-fat", serum IL-6 significantly increased at 2 and 6 hours after operation when compared with "E-gl". In "E-EPA", postoperative IL-6 was reduced and cell-mediated immune function was improved significantly.Experiment 4 : In 5 esophagectomized patients fed enterally with EPA methyl ester, ConA and PHA stimulated lymphocyte proliferation, NK activity and CD4/8 were improved significantly during the chemo-radiation therapy when compared with the patients without EPA (n= 14).Conclusion : N-6 PUFA amplified, and n-3 PUFA reduced the stress response and immunosuppression induced by burn injury or surgical stress. N-3 PUFA is potentially beneficial for the critically ill patients. Less

研究n-6和n-3多不饱和脂肪酸（PUFA）对大鼠蛋白质代谢、细胞免疫、细胞因子和前列腺素产生的影响（总能量的20%;组“N6”）、19%的红花油和1%的结构化EPA和DHA（2：1）（“N3”）和不含脂肪（“对照”），并进行20%全层烧伤。当与“对照”相比时，“N6“显示血清IL-6和TNFa增加（p<0.05），并且氮平衡（NB）降低。N3组TNF、IL-10水平降低，NB改善。实验二：43只大鼠，分别给予含%红花油/ %鱼油脂肪乳（0/0、0/20、4116、10/10、20/0）的TPN，观察其对烧伤后迟发型超敏反应（DTH）的抑制作用。DTH、GM-CSF和EPA随鱼油静脉给药剂量的增加而增加，肠道内毒素移位减少 ...更多信息 l乳液。实验3：6例食管切除患者接受无脂肪TPN（“E-gl”），6例接受含Intralidine（总能量的20%，“E-fat”）的TPN。EPA甲酯（1.8g/天）口服或肠内给予7名接受TPN与Intralidid（“E-EPA”）的患者。“E-fat”组术后2、6小时血清IL-6水平较“E-gl”组明显升高。实验4：5例食管癌切除术后给予EPA甲酯、ConA和PHA治疗的患者，与未给予EPA治疗的患者（n= 14）相比，在放化疗过程中给予EPA甲酯、ConA和PHA刺激的淋巴细胞增殖、NK活性和CD_（4/8）均明显提高。N-6 PUFA可增强烧伤或手术应激引起的应激反应和免疫抑制，而n-3 PUFA可减轻烧伤或手术应激引起的应激反应和免疫抑制。N-3 PUFA对危重患者有潜在的益处。少

项目成果

Tsuguhiko Tashiro, Ishiodori K, Yamamori H, Takagi K, Nakajima N: "Glucose oxidation in critically ill patients measured with stable isotope and indirect calorimetry" Chiba Med J. (in press). (1998)

Tsuguhiko Tashiro、Ishiodori K、Yamamori H、Takagi K、Nakajima N：“用稳定同位素和间接量热法测量危重患者的葡萄糖氧化”Chiba Med J.（出版中）。

K.Furukawa, Tsuguhiko Tashiro, H.Yamamori, K.Takagi, Y.Morishima, T.Sugiura, Y.Ootsubo, Naganori Hayashi, T.Itabashi, W.Sano, Y.Toyoda, H.Nitta, H.Nitta, and N.Nakajima: "Effects of soybean oil emulsion and eicosapentaenoic acid (EPA) on stress response a

K.Furukawa、Tsuguhiko Tashiro、H.Yamamori、K.Takagi、Y.Morishima、T.Sugiura、Y.Ootsubo、Naganori Hayashi、T.Itabashi、W.Sano、Y.Toyoda、H.Nitta、H.Nitta、

Toshiyuki Sugiura,Tsuguhiko Tashiro,Hideo Yamamori,Kazuya Takagi,Yuichi Morishima,Naoshi Shimoda,Toshiharu Ishizuka,Masamitsu Tatibana,Isao Itou,and Nobuyuki Nakajima.: "Effect of IGF-1 on endotoxin translocation in burned rats receiving TPN." Nutrition.

Toshiyuki Sugiura、Tsuguhiko Tashiro、Hideo Yamamori、Kazuya Takagi、Yuichi Morishima、Naoshi Shimoda、Toshiharu Ishizuka、Masamitsu Tatibana、Isao Itou 和 Nobuyuki Nakajima.：“IGF-1 对接受 TPN 烧伤大鼠内毒素易位的影响。”

Tsuguhiko Tashiro,H.Yamamori,N.Hayashi,K.Furukawa,Y.Morishima,T.Sugiura,T.Itabashi,W.Sano,Y.Toyoda,H.Nitta,J.Hirano,K.Nishiya and N.Nakajima,M.D.: "N-3 versus N-6 polyunsaturated fatty ascids(PUFA) in critical illness." Nutrition. 14. 1998 (551-553)

田代嗣彦、H.山森、N.Hayashi、K.Furukawa、Y.Morishima、T.Sugiura、T.Itabashi、W.Sano、Y.Toyoda、H.Nitta、J.Hirano、K.Nishiya 和 N.Nakajima

豊田康義、田代亜彦、山森秀夫、高木一也、林永規、古川勝規、板橋輝美、佐野渉、新田 宙、西谷慶、平野純子、中島伸之: "食道癌手術患者における経腸栄養法の有用性の検討." 日本静脈・経腸栄養研究会誌. 13. 230-233 (1998)

Yasuyoshi Toyoda, Ahiko Tashiro, Hideo Yamamori, Kazuya Takagi, Eiji Hayashi, Katsunori Furukawa, Terumi Itabashi, Wataru Sano, Hiroshi Nitta, Kei Nishitani, Junko Hirano, Nobuyuki Nakajima：“肠内营养在接受食管癌手术的患者中的有用性。A性别研究。”日本静脉肠内营养研究会杂志。13. 230-233 (1998)