Induction of Tumor-specific Cytotoxic Tlymphocytes from Regional Lymph Node Lymphocytes of Human Breast Cancer

点击翻译按钮获取中文摘要

In this study, we have induced the breast cancer-specific cytotoxic T lymphocytes (CTL) from regional lymph node lymphocytes (RLNL) of HLA A2-positive patients with breast cancer. Freshly isolated RLNL were stimulated with solid phase anti-CD3 monoclonal antibody followed by expansion with recombinant interleukin-2. Subsequently, the RLNL were stimulated with an irradiated HLA 0201 breast cancer cell line, MCF-7, at responder/stimulator ratio of 10/1 once a week for 2 weeks. The cultured RLNL exhibited specific lysis against MCF-7 in all 5 HLA A2-positive patients tested, but not in 2 HLA A2-negative patients. The cytotoxicity against MCF-7 was substantially inhibited by an addition of anti-HLA A2 mAb. In 3 out of the 5 HLA A2-positive patients, the anti-MCF-7 CTL also exhibited a substantial level of reactivity against PC-9, an HLA A0206-positive lung adenocarcinoma cell line. Inversely, anti-PC-9-speciflc CTL was inducible by multiple stimulations of the RLNL with PC-9 cells in 2 out of the 3 patients. These results suggested that several common tumor antigens might exist among HLA A2-positive breast cancers, some of which may be shared with lung adenocarcinomas.