p16 Expression Adenovirus Vector for Esophageal and Pancreas Cancer Therapy.

“肺癌患者在基础外科手术后也出现了糟糕的症状，而辅助治疗也有必要改善外科结果。”P16イD1 INK4aイエD1 （p16） is tight-binding and inhibitory protein for CDK4 to induce G1 arrest of cell cycle。P16基因缺失在人类肺癌中经常被识别。P16的受损基因功能可能是肺癌细胞不受控制的生长和生长的主要因素。在这项研究中，我们调查了腺病毒P16表达载体对肺癌细胞增殖的影响，无论是澄清还是不确定载体可能是一种承诺模式，以协助肺癌的外科治疗。我们构建了腺病毒P16表达载体（AdexCACP 16）将p16 cDNA插入含有E1和E3缺失的盒Cosmid的5型腺病毒基因组。因此，我们评估了AdexCACP 16的活动，以诱导p16基因mRNA表达在肺癌细胞线、MIAPaCa-2中的活性，并控制细胞增殖。AdexCACP 16诱导MIAPaCa-2细胞中的p16基因mRNA表达的高水平与细胞接触1小时。该细胞的增加被AdexCASP 16与控制腺病毒组比较明显地抑制，这些数据指示了AdexCASP 16有潜力诱导p16基因表达和控制肺癌细胞增殖，而腺病毒p16表达载体AdexCASP 16可能是一种可行的基因治疗方法，以改善肺癌的外科治疗结果。

The prognoses of pancreas cancer patients have been miserable even after radical surgery, and the adjuvant therapy is necessary to improve the surgical results. P16ィイD1INK4aィエD1 (p16) is tight-binding and inhibitory protein for CDK4 to induce G1 arrest of cell cycle. P16 gene deletion is frequently identified in human pancreas cancer. The impaired gene function of p16 might be a major factor of the uncontrolled proliferation and malignancy of pancreas cancer cells. In this study, we investigated the effect of adenovirus p16 expression vector for pancreas cancer cell proliferation to clarify whether or not the vector might be a promising mode to assist the surgical therapy for pancreas cancer.First, we constructed the adenovirus p16 expression vector (AdexCACSp16) by inserting p16 cDNA to a cassette cosmid containing a nearly full-length adenovirus type 5 genome with E1 and E3 deletions. Thereafter, we assessed the activity of AdexCACSp16 to induce p16 gene mRNA expression in pancreas cancer cell line, MIAPaCa-2 and to control the cell proliferation.AdexCACSp16 induced the high level of p16 gene mRNA expression in MIAPaCa-2 cells with 1 hour contact to the cells. The cells proliferation was significantly suppressed by AdexCACSp16 compared with the control adenovirus group.These data indicated that AdexCACSp16 has the potential to induce p16 gene expression and control pancreas cancer cell proliferation and that the adenovirus p16 expression vector AdexCACSp16 might be a possible method of gene therapy to improve the surgical therapeutic results for pancreas cancer.