Basic Study on transplantation of Langerhans islets into the liver via hepatic artery in animals.

朗格汉斯胰岛经肝动脉移植入动物肝脏的基础研究。

• 批准号: 02670591
• 负责人: KOBAYASHI Susumu
• 金额: $ 1.28万
• 依托单位: The Jikei University School of Medicine.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670591/
• 关键词: rat; pancreatic islets; hepatic artery; transplantation; 膵臓移植; SD系雄性ラット

At the beginning of the experiment, an experimental model was prepared by adult hybrid dogs but liberation of pancreatic cells of dogs was difficult making it impossible to continue the experiment. Therefore, the experimental animals were changed from hybrid dogs to SD strain male rats. Pancreatic cells of rats were relatively easily liberated and many free cells were able to be recovered. However, the hepatic artery of a rat was too fine to permit planned infusion of free pancreatic cells and therefore it took time to establish the technique. Free pancreatic colls were successfully infused into the liver by infusing them into the gastroduodenal artery after cramping the common hepatic artery and gastroduodenal artery. It was also successful to visually confirm the pancreatic cells had survived. The confirm this, diabetic rats were used. Diabetic rats were prepared by intravenous injection of streptozotocin.It will be necessary, after this, to confirm the survival of transplanted pancreatic cells using diabetic rats.Besides that, the end to side anastomosis of the portal vein and inferior vena cava and transposition of the portal vein and inferior vena cava were also successful in an animal model.After this, it will be necessary to transplant free pancreatic cells into the liver via the hepatic artery using the planned animal model (end to side anastomosis of the portal vein and inferior vena cava and transposition of the portal vein and inferior vena cava) in order to demonstrate the effect of the hepatotrophic factor secreted from pancreatic cells as a regenerative factor of the liver.

在实验开始时，用成年杂种狗制备实验模型，但狗的胰腺细胞的释放很困难，使得不可能继续实验。因此，将实验动物由杂种犬变更为SD系雄性大鼠。大鼠胰腺细胞相对容易释放，并且能够回收许多游离细胞。然而，大鼠的肝动脉太细，不允许有计划地输注游离胰腺细胞，因此建立该技术需要时间。在夹闭肝总动脉和胃十二指肠动脉后，通过将游离胰结肠注入胃十二指肠动脉成功地将游离胰结肠注入肝脏。还成功地目视确认了胰腺细胞存活。为了证实这一点，使用了糖尿病大鼠。通过静脉注射链脲佐菌素制备糖尿病大鼠。之后，需要确认使用糖尿病大鼠移植的胰腺细胞的存活。此外，门静脉和下腔静脉的端侧吻合和门静脉和下腔静脉的移位在动物模型中也是成功的。之后，必须使用计划的动物模型通过肝动脉将游离胰腺细胞移植到肝脏中（门静脉-下腔静脉端侧吻合术和门静脉-下腔静脉转位术）以证明从胰腺细胞分泌的肝营养因子作为肝再生因子的作用。