Local Chemotherapy via Hepatic Arterial and Portal Blood Flows For Liver Cancer

通过肝动脉和门静脉血流进行局部化疗治疗肝癌

• 批准号: 09671286
• 负责人: GOSEKI Narihide
• 金额: $ 1.73万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671286/
• 关键词: Local Chemotherapy; Hepatic ARterial Chemotherapy; Portal Vein; Liver Cancer; Chemotherapy via Hepatic Arterial and Portal blood Flows; 肝動注; 抗癌剤; 局所化学療法; 肝動脈内注入; 門脈内注入; ドキソルビジン; 家兎; VX2腫瘍

(a) Via the hepatic artery of the rabbits inoculated VX2 tumor, ADM at a dose of 1mg/kg was administered and GSH was also infused via the portal vein at the same time (Gl(+)group (n=6)). As a control group, the rabbits were administered only ADM without GSH infusion (Gl(-)group (n=6)). Then the ADM concentrations in the liver tissue in both groups were analyzed seriously. Before and 5, 10, 15, 20, 25, 30 and 45 minutes after the ADM administration, hepatic tissue and the blood in the caval vein were sampled and measured the ADM concentration by the HPLC method.(b) In the same models, anticancer effect in both groups were compared.(c) (Results) (a) (1) The ADM concentrations showed the same values, with the highest in 5 to 10 minutes after ADM administration in both Gl(+) and Gl(-) groups.(2) The ADM concentrations in the liver of Gl(+) were higher than those of Gl(-) group.(3) There was no difference between the ADM concentrations in the blood in Gl(+) and Gl(-) groups.(b) Median survival day of the animals in the Gl(+) group was 18.6±7.3, and that of Gl(-) was 16.4±6.3 days.

(a)G1（+）组（n=6）：经肝动脉注入阿霉素（ADM）1 mg/kg，同时经门静脉注入谷胱甘肽（GSH）。对照组（Gl（-）组，n=6），只给予ADM，不输注GSH。并对两组大鼠肝组织中ADM浓度进行统计学分析。于给药前及给药后5、10、15、20、25、30、45 min分别取肝组织和腔静脉血，用HPLC法测定ADM浓度。(b)在相同的模型中，比较两组的抗癌效果。(c)结果（a）（1）G1（+）组和G1（-）组ADM浓度在给药后5 ~ 10 min达到最高值; (2)G1（+）组肝组织中ADM浓度高于G1（-）组。(3)Gl（+）组与Gl（-）组血中ADM浓度无显著性差异。(b)Gl（+）组动物的中位生存天数为18.6±7.3天，Gl（-）组为16.4±6.3天。