Roles of cytokines for liver regeneration in hepatectomy rat models with postoperative infection

细胞因子对肝切除大鼠术后感染模型肝再生的作用

基本信息

  • 批准号:
    09671311
  • 负责人:
  • 金额:
    $ 2.18万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1997
  • 资助国家:
    日本
  • 起止时间:
    1997 至 1998
  • 项目状态:
    已结题

项目摘要

1 : Hepatic regeneration after hepatectomy and endotoxin administrationPostoperative infection inhibits liver regeneration after hepatectomy. To elucidate this inhibition, we analyzed growth rates of hepatocyte and signals between Kupffer cells and hepatocyte using rat 70% hepatectomy model with or without endotoxin (ET) administration. In this rat postoperative infection model, we administered ET (1 mg/kg) one day after hepatectomy. To evaluate hepatocyte growth rate, PCNA (proliferative core nuclear antigen) staining was performed in liver specimens obtained at 3 and 7 postoperative days. The percentages of PCNA positive cells were 27% and 17% in the rats with only hepatectomy whereas those were 16% and 10 % in 1 mg/kg ET-administered rats, respectively. Thus, ET administration inhibited liver regeneration after hepatectomy.2. Molecular analysis for signalings of hepatic regenerationUsing the same hepatectomy model rats with low dose ET (30 mug/kg)and high dose ET (1 mg/kg)-administe … More red rats, we measured HGF (hepatocyte growth factor) mRNA and c-met mRNA expressions in the liver specimens obtained 3,6,12,24,48 and 72 hours after hepatectomy. in the rats with only hepatectomy, HGF expression increased 12-24 hours after hepatectomy and c-met expression simultaneously increased. In the low dose ET administered rats, the expression levels of HGF and c-met were higher than those in the rats without ET-administration. On the other hand, in the high dose ET administered rats, peak of HGF expression was delayed and unlikely to cooperate with c-met expression.These data suggested that low dose administration of ET after hepatectomy stimulated hepatic regeneration after hepatectomy, whereas its high dose administration inhibited. ET administration stimulated inflammatory cytokines by Kupffer cells. The overproduction of inflammatory cytokines due to postoperative severe infection might disarrange signal transduction for hepatocyte growth, which causes the delay of liver regeneration and increases the death rates of these rats model. Less
1:肝切除术后的肝再生和内毒素给药术后感染抑制肝切除术后的肝再生。为了阐明这种抑制作用,我们分析了肝细胞的生长速率和枯否细胞和肝细胞之间的信号,使用大鼠70%肝切除模型,有或没有内毒素(ET)的管理。在这个大鼠术后感染模型中,我们在肝切除术后第一天给予ET(1 mg/kg)。为了评估肝细胞生长率,在术后3天和7天获得的肝脏标本中进行PCNA(增殖核心核抗原)染色。PCNA阳性细胞百分比在仅肝切除的大鼠中分别为27%和17%,而在1 mg/kg ET给药的大鼠中分别为16%和10%。因此,ET给药抑制了肝切除后的肝再生。肝再生信号的分子分析采用同一肝切除模型大鼠,给予低剂量ET(30 μ g/kg)和高剂量ET(1 mg/kg), ...更多信息 方法:采用健康雄性SD大鼠,于肝切除术后3、6、12、24、48和72 h取肝组织,检测肝细胞生长因子(HGF)mRNA和c-met mRNA的表达。在仅进行肝切除的大鼠中,肝切除后12-24小时HGF表达增加,同时c-met表达增加。低剂量ET组大鼠肝细胞HGF和c-met表达水平高于对照组。高剂量ET组HGF表达高峰延迟,且与c-met表达无协同作用,提示肝切除术后给予低剂量ET可促进肝再生,而高剂量ET则抑制肝再生。ET刺激枯否细胞产生炎性细胞因子。术后严重感染引起的炎性细胞因子的过度产生可能会扰乱肝细胞生长的信号传导,导致肝再生延迟,增加模型大鼠的死亡率。少

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takesue Y: "Prediction for the development of postoperative infections in the operation of esophageal cancer compared with gastric surgery." Hiroshima J Med Sci.47・3. 109-113 (1998)
Takesue Y:“与胃手术相比,食道癌手术中术后感染的发生情况预测” Hiroshima J Med Sci.47・3(1998)。
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    0
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横山隆: "消化管不全" 救急医学. 22・13. 1954-1958 (1998)
横山隆:“胃肠功能不全”急诊医学。 22・13 1954-1958(1998)
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    0
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村上義昭: "各種侵襲下におけるサイトカインと臓器障害との関連" Biotherapy. 12・5. 604-606 (1998)
村上义明:“各种侵袭下细胞因子与器官损伤的关系”生物治疗12・5(1998)。
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    0
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山東敬弘: "サイトカイン誘導能よりみた消化器外科術後MRSA腸炎起炎菌の実験的検討" 日消外会誌. 30・7. 1734-1738 (1997)
Takahiro山东:“从细胞因子诱导能力的角度对胃肠道手术后引起MRSA肠炎的细菌进行实验研究”《日商国外杂志》30・7(1997)。
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
横山隆: "臨床浸襲学" 小川道雄、斉藤英昭編, 62-70 (1998)
Takashi Yokoyama:“临床侵袭研究”,小川道雄和齐藤秀明编辑,62-70 (1998)
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YOKOYAMA Takashi其他文献

YOKOYAMA Takashi的其他文献

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{{ truncateString('YOKOYAMA Takashi', 18)}}的其他基金

Inhibition of novel cancer specific antioxidant regulation promotes anticancer function
抑制新型癌症特异性抗氧化调节可促进抗癌功能
  • 批准号:
    18K07232
  • 财政年份:
    2018
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Organization and Activity of Voluntary Group for Crime Control and Community Type
犯罪控制及社区类型志愿小组的组织和活动
  • 批准号:
    23730812
  • 财政年份:
    2011
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Functional analysis of Trib1/2 double knockout mice on hematopoiesis
Trib1/2双敲除小鼠造血功能分析
  • 批准号:
    23790451
  • 财政年份:
    2011
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Evaluation of impact fracture characteristics of laminated composites : enhancement of measurement accuracy
层状复合材料冲击断裂特性的评估:测量精度的提高
  • 批准号:
    22560686
  • 财政年份:
    2010
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation of STM induced light emission from single molecules
单分子 STM 诱导光发射的研究
  • 批准号:
    21360024
  • 财政年份:
    2009
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Leukemogenic activity of Trib1
Trib1 的致白血病活性
  • 批准号:
    21790333
  • 财政年份:
    2009
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
The people's social control to crime and delinquency as event
人民群众对犯罪违法行为的社会控制
  • 批准号:
    19730529
  • 财政年份:
    2007
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Formation Region of Monophase with Cubic SpineL-type Structure in Mn-Fe-Ni Ternary System and Electrical Properties of Sintered-bodies Prepared from the Region
Mn-Fe-Ni三元系中立方脊柱L型结构单相的形成区域及该区域制备的烧结体的电性能
  • 批准号:
    17560593
  • 财政年份:
    2005
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
CLINICAL USE OF MOLECULAR EPIDEMIOLOGY FOR NOSOCOMIAL IMIPENEMRESISTANT PSEUDOMONAS AERUGINOSA INFECTIONS
分子流行病学在院内亚胺培南耐药铜绿假单胞菌感染中的临床应用
  • 批准号:
    11671163
  • 财政年份:
    1999
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The study with rapid detection for the toxins of Methicillin-resistant Staphylococcus aureus and with the action of the toxins
快速检测耐甲氧西林金黄色葡萄球菌毒素及其作用的研究
  • 批准号:
    04670733
  • 财政年份:
    1992
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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Indoor endotoxin concentrations and the risk of airborne infection and allergy symptoms
室内内毒素浓度与空气传播感染和过敏症状的风险
  • 批准号:
    23H01573
  • 财政年份:
    2023
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    $ 2.18万
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Aiming at a new target in endotoxin-associated lung damage: The Ig domain 3 of ICAM-1
瞄准内毒素相关肺损伤的新靶点:ICAM-1 的 Ig 结构域 3
  • 批准号:
    479050
  • 财政年份:
    2023
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    $ 2.18万
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    Operating Grants
Out of Time: Biomedicine, Endotoxin Detection, and the Plight of the Horseshoe Crab
过时的:生物医学、内毒素检测和鲎的困境
  • 批准号:
    10577633
  • 财政年份:
    2023
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    $ 2.18万
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Development of a novel strategy of circulatory management of septic shock with 2-Methyl-2thiazoline
使用 2-甲基-2噻唑啉开发感染性休克循环管理新策略
  • 批准号:
    23K08452
  • 财政年份:
    2023
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Endotoxin Induced Cardiac Mitochondrial Damage
内毒素引起的心脏线粒体损伤
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    573265-2022
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    2022
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    University Undergraduate Student Research Awards
Aiming at a novel target in endotoxin-associated lung damage: The Ig domain 3 of ICAM-1
针对内毒素相关肺损伤的新靶点:ICAM-1 的 Ig 结构域 3
  • 批准号:
    477251
  • 财政年份:
    2022
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    $ 2.18万
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    Operating Grants
Assessing the Behaviour of Endotoxin in Biopharmaceutical Formulations Lowering the Likelihood of Drug Induced Toxic Shock in Patients
评估生物制药制剂中内毒素的行为,降低患者药物引起中毒性休克的可能性
  • 批准号:
    BB/V50936X/1
  • 财政年份:
    2021
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    $ 2.18万
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Does endotoxin administration increase alcohol consumption in individuals with AUD?
内毒素给药是否会增加 AUD 患者的饮酒量?
  • 批准号:
    10403489
  • 财政年份:
    2021
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    $ 2.18万
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AlphaBET - Endotoxin detection for the 21st Century
AlphaBET - 21 世纪的内毒素检测
  • 批准号:
    830248
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    2021
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    $ 2.18万
  • 项目类别:
    Innovation Loans
Elucidation of physiochemical characteristics and control measures of endotoxin-producing bacteria dominating in water purification systems
净水系统中优势产内毒素菌的理化特性及控制措施的阐明
  • 批准号:
    21H01469
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    2021
  • 资助金额:
    $ 2.18万
  • 项目类别:
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