Growth factors and adhesion molecules in the progression of metastasizing capability of cancer cell lines.

生长因子和粘附分子在癌细胞系转移能力进展中的作用。

• 批准号: 09671332
• 负责人: KAI Toshihiro
• 金额: $ 0.51万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671332/
• 关键词: ER-1; ER-pP; TGF-β; TGF-β receptor; VEGF; 癌転移; ER-1細胞

A rat breast cancer cell line ER-1, which cannot metastasize to the lung, forms lung metastatic lesions when it is cultured with TGF-beta 1. And three other cell lines (ER-pP1, -pP2, -pP3), derived from ER-1 by foreign substance reaction, make severe metastatic lesions to the lung. We studied about the growth factors and adhesion molecules in the difference of metastasizing capability of these cancer cell lines. ER-1 expressed the TGF-beta type I and type II receptors immunohistochemically, however other metasatable cell lines did not. And ER-1 cell count increased partially in soluble TGF-beta type II receptor treatment. These findings suggests that the TGF-beta may play a role of metastatic capability in these cell lines. All of these cell lines expressed VEGF responsibly with hypoxic stimulation in northern blotting. ER-1 treated with TGF-beta 1 for 30 days expressed increased level of TGF-beta 1 and VEGF in northern blotting. And there was not differential expression of E-cadherin and PCNA in both cells immunohistochemically. These results suggest that the difference of matastatic capability of these cell lines may concerned with TGF-beta and VEGF. We now continue to study the difference between these cell lines by molecular biological analysis.

不能转移到肺的大鼠乳腺癌细胞系ER-1在与TGF-β 1一起培养时形成肺转移病灶。另外3株由ER-1通过外源性反应衍生的细胞系（ER-pP 1、-pP 2、-pP 3）也可造成严重的肺转移灶。我们研究了生长因子和粘附分子在这些肿瘤细胞株转移能力差异中的作用。ER-1通过免疫组织化学方法表达TGF-β I型和II型受体，但其他可转移细胞系则不表达。可溶性TGF-β II型受体治疗后ER-1细胞计数部分增加。这些发现表明TGF-β可能在这些细胞系中发挥转移能力的作用。北方印迹法显示所有细胞系在低氧刺激下均表达VEGF。北方印迹显示，TGF-β 1处理30 d后，ER-1表达TGF-β 1和VEGF水平增加。E-cadherin和PCNA在两种细胞中的表达无明显差异。结果提示，不同细胞系的转移能力差异可能与TGF-β和VEGF有关。我们现在继续通过分子生物学分析来研究这些细胞系之间的差异。

项目成果

T. Kai, et al.: "Cytokines, Cholera, and the Gut."IOS press. Amsterdam. 362 (1997)

T. Kai 等人：“细胞因子、霍乱和肠道。”IOS 出版社。