Tolerance Induction on Experimental Cardiae Xenotransplantation

实验性心脏异种移植的耐受诱导

• 批准号: 09671356
• 负责人: YOKOYAMA Hitoshi
• 金额: $ 1.92万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671356/
• 关键词: Heart transplantation; Xenotransplantation; Tolerance; 心臓異種移植; 胸腺; 心臓移殖; 異種移殖

Background : Innoculation of allo-antigens in the recipient's thymus successfully induces long-ram tolerance on experimental cardiac transplantation in rodents.Aim : We investigated the effects of xeno-antigen innoculation in the recipient's thymus on the survival cardiac xenotransplantation in rats.Methods : Heterotopic abdominal transplantation model was employed using Golden Syrial Hamster as a donor of heart and splenocyte and Lewis rat as a recipient. On day-1, antilympocyte serum was injected to recipient, followed by intrathymic innoculation of donor splenocyte on the day 0. On the day 21, heart transplantation was performed from Hamster rat.Result : 1) Untreated recipient rejected the transplanted heart in 3 days (control). 2) Transplanted heart in rat were rejected in a same time frame. 3) Transplanted heart in the treated adult rat were rejected in a hour thyperacute rejection). 4) Whole body irradiation (WBI) before thymic inoculation prevented hypercute rejection seen in the untreated adult rat, with a mean graft survival time of 3 days.Conclusion : In contrast to allografm transplantation. thymic innoeufation of donor splenocytes does not induce toleranceto cardiac xenograft in the Hamster to rat model. In addition the thymic innoculation with xenoanmigen rather inducehyperacute rejection of cardiac xenograft. Using neonate as a recipient, WBI and cycfophosphoamide prevented the hyperacute rejection, however, failed to elongate graft survival. We conclude different immune reaction take place after intrathmic inoculation of xeno-antigen in comparison with that of also-antigen.

背景：接收者的胸腺中同种抗原的固结成功地诱导了啮齿动物中实验性心脏移植的长镜头。心脏和脾细胞和刘易斯大鼠的供体作为接受者。在第1天，将抗脑细胞血清注射到受体中，然后在第0天对供体脾细胞的胸膜固定。在第21天，从仓鼠大鼠进行心脏移植。从仓鼠大鼠进行：1。 2）在同一时间范围内拒绝大鼠中移植的心脏。 3）在一个小时的thyperacute拒绝中，拒绝了经过治疗的成年大鼠的心脏移植心脏）。 4）胸腺接种之前的全身照射（WBI）阻止了未处理的成年大鼠中观察到的过度排斥，平均移植物存活时间为3天。结论：与Allografmafm移植相反。供体脾细胞的胸腺innoefation不会在大鼠模型中诱导仓鼠中的toleranceto心脏异种移植。另外，异种狂热的胸腺固定是心脏异种移植物的诱导性抑制。但是，使用新生儿作为接受者，WBI和环磷有酰胺阻止了超急性排斥反应，但未能延长移植物的存活率。与抗原抗原相比，我们得出结论在异抗抗原的固有接种后发生不同的免疫反应。

项目成果

Yokoyama H et al: "Experimental cardial xenografting with irradiation・・・" Transplant Proc.

Yokoyama H 等人：“辐射实验性心脏异种移植......”移植过程。

Yokoyama H et al.: "Tolerance to experimental cardial allografts but net・・・" Transplant Proc.

Yokoyama H 等人：“对实验性心脏同种异体移植物的耐受性，但净……”移植过程。

Yokoyama H,Iguchi A,Ohmi M,Tabayashi K.: "Experimental cardiac xenografting with irradiation and intrathymic xenogeneic cells." Transplant Proc. (in press).

Yokoyama H、Iguchi A、Ohmi M、Tabayashi K.：“利用辐射和胸腺内异种细胞进行心脏异种移植实验。”

Yokoyama H,Iguchi A,Ohmi M,Tabayashi K.: "Tolerance to experimental cardiac allografts but not xenografts is induced after simultaneous neonatal intrathymic inoculation with allo-and xenogeneic cells." Transplant Proc. (in press).

Yokoyama H、Iguchi A、Ohmi M、Tabayashi K.：“在新生儿胸腺内同时接种同种异体和异种细胞后，会诱导对实验性心脏同种异体移植物的耐受性，但不会诱导异种移植物的耐受性。”