A study of the mechanism of immune induced central nervous system anomaly

抗妊娠大鼠肾血清(ARKS)引起的中枢神经系统异常。为了阐明免疫诱导大鼠异常的机制，我们研究了几个类别。在癌基因的相关研究中，我们没有发现S-myc、c-、nyc、fas和Rb的表达，只有野生型P53表达，认为该抑制基因可能被ARKS诱导修复受损的细胞核。在肿瘤生长因子方面，Mib-L和Ki-67不表达，只有对照组比注射抗血清的对照组有更高的增殖细胞核抗原表达。提示ARKS对神经管上皮细胞生长有抑制作用。此时未检测到染色体异常。如果你认为妊娠是一种器官移植，有一些细胞免疫的作用。在此模型中，我们研究了细胞免疫的关系。T细胞、B细胞和…抗原注射ARKS组大鼠子宫、胎盘、羊膜愈合处可见较多巨噬细胞。细胞免疫不是直接作用于ARKS处理的胎儿，而是间接作用于胎儿。用荧光免疫组织化学方法研究刀豆蛋白A(ConA)和小麦胚凝集素(WGA)两种凝集素与神经上皮细胞糖结合物的结合。结果发现，在妊娠第9天(GD9)，在神经管的基底膜和细胞间区的神经上皮腔内可见荧光。在GD21胚胎中，注射ARKS的大鼠胚胎在神经发育过程中神经上皮细胞表面的细胞外基质和室管膜衬里细胞的荧光强度明显高于注射NRS的大鼠胚胎。这些结果支持简单的闭合和过度生长是神经管缺陷的机制。细胞凋亡与中枢神经系统异常表达的关系目前尚不清楚。因为Fas和bcl2在母体和胎儿组织中的免疫组织化学检查均为阴性。较少

The central nervous system anomalies induced by anti-pregnant rat kidney serum (ARKS). In order to clarify the mechanism of immune induced anomalies in rat, we studied several numbers of categories. The results of study related to oncogene, we could not demonstrate an expression of s-myc, c-, nyc, fas and RB.Only wild type of P53 was expressed, and it was considered that this supressor gene may be induced to repair the damaged nuclei by ARKS.On the tumor growth factor, Mib-l and Ki-67 did not express and only PCNA revealed higher in control which were injected saline instead of antiserum than ARKS injected rats. This results suggested that the ARKS inhibit the growth of epithelial cells of the neural tube. The abnomality of chromosome was not detected at this time. If you may consider that the pregnancy is one of organ transplantation, there is some action of cell mediated immunity. We studied the relation of cell mediated immunity in this rat model. The antigens of T-cell, B-cell, and … More macrophages were demonstrated in uterus, placenta, amunion of ARKS injected rats group. The cell mediated immunity did not act directry but act indirectry to the fetus which treated by ARKS.Fluorescence immunohistochemical studies were performed to localize the binding of two lectins, concanavalin A (Con A) and wheat germ agglutinin (WGA), to glycoconjugates on neuroepithelium. We found fluorescence was observed on the luminal side of the neuroepithelium in the intercell- ular region and on the basement membrane of the neural tube in embryos on day 9 of gestation (GD 9). In GD 21 embryos there was much more intense fluorescence in the extracellular matrix and the ependymal lining cells of the cell surface of the neuroepithelium during neurulation was different in rat embryos injected with ARKS than in normal embryos injected with NRS.These results support the idea that simple nonclosure and overgrowth constitute the mechanism of neural tube defect. The relation of apoptosis in expression of CNS anomaly is not clear at present. Because, fas and bcl-2 was totally negative in immunohistochemical examinations on the both mother and fetal tissues. Less