A study of the mechanism of immune induced central nervous system anomaly

免疫诱发中枢神经系统异常的机制研究

• 批准号: 09671427
• 负责人: KURISAKA Masahiro
• 金额: $ 2.11万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671427/
• 关键词: congenital hydrocephalus; humoral & cell medical immunity; oncogene; concanavalin A (Con A); wheat germ agglutinin (WGA); apotosis; chromosome; Mib-1; antibody; PCNA; Wistar rat; humoralimmunity; cell mediateimmunity; concanavalinA; wheat ge

The central nervous system anomalies induced by anti-pregnant rat kidney serum (ARKS). In order to clarify the mechanism of immune induced anomalies in rat, we studied several numbers of categories. The results of study related to oncogene, we could not demonstrate an expression of s-myc, c-, nyc, fas and RB.Only wild type of P53 was expressed, and it was considered that this supressor gene may be induced to repair the damaged nuclei by ARKS.On the tumor growth factor, Mib-l and Ki-67 did not express and only PCNA revealed higher in control which were injected saline instead of antiserum than ARKS injected rats. This results suggested that the ARKS inhibit the growth of epithelial cells of the neural tube. The abnomality of chromosome was not detected at this time. If you may consider that the pregnancy is one of organ transplantation, there is some action of cell mediated immunity. We studied the relation of cell mediated immunity in this rat model. The antigens of T-cell, B-cell, and … More macrophages were demonstrated in uterus, placenta, amunion of ARKS injected rats group. The cell mediated immunity did not act directry but act indirectry to the fetus which treated by ARKS.Fluorescence immunohistochemical studies were performed to localize the binding of two lectins, concanavalin A (Con A) and wheat germ agglutinin (WGA), to glycoconjugates on neuroepithelium. We found fluorescence was observed on the luminal side of the neuroepithelium in the intercell- ular region and on the basement membrane of the neural tube in embryos on day 9 of gestation (GD 9). In GD 21 embryos there was much more intense fluorescence in the extracellular matrix and the ependymal lining cells of the cell surface of the neuroepithelium during neurulation was different in rat embryos injected with ARKS than in normal embryos injected with NRS.These results support the idea that simple nonclosure and overgrowth constitute the mechanism of neural tube defect. The relation of apoptosis in expression of CNS anomaly is not clear at present. Because, fas and bcl-2 was totally negative in immunohistochemical examinations on the both mother and fetal tissues. Less

抗孕大鼠肾血清（ARKS）引起的中枢神经系统异常。为了阐明大鼠免疫诱导异常的机制，我们研究了多个类别。与癌基因相关的研究结果，我们无法证明s-myc、c-、nyc、fas和RB的表达。仅野生型P53表达，认为该抑制基因可能被ARKS诱导修复受损的细胞核。在肿瘤生长因子上，Mib-l和Ki-67不表达，仅PCNA显示在注射生理盐水而不是抗血清的对照中较高。 ARKS注射老鼠。该结果表明ARKS抑制神经管上皮细胞的生长。此时未检测到染色体异常。如果你认为怀孕是器官移植的一种，那么细胞免疫有一定的作用。我们研究了该大鼠模型中细胞介导的免疫的关系。 ARKS注射组大鼠的子宫、胎盘、弹药中均显示出T细胞、B细胞和巨噬细胞的抗原。细胞介导的免疫不是直接作用，而是间接作用于 ARKS 治疗的胎儿。进行荧光免疫组织化学研究以定位两种凝集素，刀豆球蛋白 A (Con A) 和麦芽凝集素 (WGA) 与神经上皮上糖缀合物的结合。我们发现在妊娠第 9 天（GD 9）的胚胎中，细胞间区域神经上皮的管腔侧和神经管基底膜上观察到荧光。在GD 21胚胎中，注射ARKS的大鼠胚胎在神经形成过程中的细胞外基质和神经上皮细胞表面的室管膜衬里细胞中存在更强烈的荧光，与注射NRS的正常胚胎不同。这些结果支持简单的不闭合和过度生长构成神经管缺陷机制的观点。细胞凋亡与CNS异常表达的关系目前尚不清楚。因为，母体和胎儿组织的免疫组化检查中fas和bcl-2均为阴性。较少的

项目成果

栗坂 昌宏 他: "抗妊娠ラット腎血清誘発水頭症胎仔における細胞性免疫の関与" 神経免疫研究. 10巻. 216-220 (1997)

Masahiro Kurisaka 等人：“抗妊娠大鼠肾血清诱导的脑积水对胎儿细胞免疫的影响”《神经免疫学研究》第 10 卷，216-220（1997 年）。

Kurisaka M, et al: "The relation of cell mediated immunity in the anti-pregnant-rat-kidney serum induced hydrocephalic rat fetus." Brain and Immunity. 10. 216-220 (1997)

Kurisaka M 等人：“抗妊娠大鼠肾血清诱导的脑积水大鼠胎儿中细胞介导的免疫的关系。”