A study of the mechanism of immune induced central nervous system anomaly

抗妊娠大鼠肾血清（ARKS）致中枢神经系统异常。为了阐明大鼠免疫诱导异常的机制，我们研究了几个类别。与癌基因相关的研究结果，我们无法证实s-myc、c-、nyc、fas和RB的表达。仅表达野生型P53，认为该抑制基因可能通过ARKS诱导修复受损细胞核。在肿瘤生长因子方面，以生理盐水代替抗血清的对照组，mib - 1和Ki-67不表达，只有PCNA表达高于ARKS注射组。提示ARKS对神经管上皮细胞的生长有抑制作用。此时未检出染色体异常。如果考虑到妊娠是器官移植之一，则有一定的细胞介导免疫作用。我们研究了该大鼠模型中细胞介导免疫的关系。注射ARKS组大鼠子宫、胎盘、吻合口均可见到较多的巨噬细胞。细胞介导免疫对经ARKS处理的胎儿没有直接作用，而是间接作用。荧光免疫组织化学研究定位了两种凝集素，魔豆蛋白A （Con A）和小麦胚芽凝集素（WGA）在神经上皮上与糖结合物的结合。我们发现胚胎在妊娠第9天（妊娠第9天）在细胞间区神经上皮管腔侧和神经管基底膜上观察到荧光。在GD - 21胚胎中，注射ARKS的大鼠胚胎的细胞外基质和神经上皮细胞表面的室管膜衬里细胞的荧光强度明显高于注射NRS的正常胚胎。这些结果支持单纯不闭合和过度生长构成神经管缺损机制的观点。凋亡与中枢神经系统异常表达的关系目前尚不清楚。因为母胎组织免疫组化检查fas和bcl-2均为阴性。少

The central nervous system anomalies induced by anti-pregnant rat kidney serum (ARKS). In order to clarify the mechanism of immune induced anomalies in rat, we studied several numbers of categories. The results of study related to oncogene, we could not demonstrate an expression of s-myc, c-, nyc, fas and RB.Only wild type of P53 was expressed, and it was considered that this supressor gene may be induced to repair the damaged nuclei by ARKS.On the tumor growth factor, Mib-l and Ki-67 did not express and only PCNA revealed higher in control which were injected saline instead of antiserum than ARKS injected rats. This results suggested that the ARKS inhibit the growth of epithelial cells of the neural tube. The abnomality of chromosome was not detected at this time. If you may consider that the pregnancy is one of organ transplantation, there is some action of cell mediated immunity. We studied the relation of cell mediated immunity in this rat model. The antigens of T-cell, B-cell, and … More macrophages were demonstrated in uterus, placenta, amunion of ARKS injected rats group. The cell mediated immunity did not act directry but act indirectry to the fetus which treated by ARKS.Fluorescence immunohistochemical studies were performed to localize the binding of two lectins, concanavalin A (Con A) and wheat germ agglutinin (WGA), to glycoconjugates on neuroepithelium. We found fluorescence was observed on the luminal side of the neuroepithelium in the intercell- ular region and on the basement membrane of the neural tube in embryos on day 9 of gestation (GD 9). In GD 21 embryos there was much more intense fluorescence in the extracellular matrix and the ependymal lining cells of the cell surface of the neuroepithelium during neurulation was different in rat embryos injected with ARKS than in normal embryos injected with NRS.These results support the idea that simple nonclosure and overgrowth constitute the mechanism of neural tube defect. The relation of apoptosis in expression of CNS anomaly is not clear at present. Because, fas and bcl-2 was totally negative in immunohistochemical examinations on the both mother and fetal tissues. Less