Biochemical and clinical studies of melatonin secretion of the brain tumors in pineal region.

松果体区脑肿瘤褪黑激素分泌的生化和临床研究。

• 批准号: 01570816
• 负责人: KURISAKA Masahiro
• 金额: $ 1.28万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570816/
• 关键词: Pineal body; Melatonin; Pineal cell tumor; Germ cell tumor; Circadian rhythm; Immunocytochemistry; 胚芽腫; 水頭症; 髄液

The changes in daytime levels of melatonin (MLT) in the cerebrospinal fluid (CSF) of twenty seven hydrocephalic patients were studied by the high-performance liquid chromatiography (HPLC) method. Patients comprised three with congenital hydrocephalus (spina bifida 1, Chiari type II malformation 2), four postmeningitic hydrocephalus, fifteen brain tumors (chiasmal germinoma 3 ; malignant glioma of frontal 3, and temporal lobes 1 ; germinoma 1, teratoma 2, yolk sac tumor 1, epidergerminoma 1, teratoma 2, yolk sac tumor 1, epidermoid 1 in pineal region) and five cases of normal pressure hydrocephalus. CSF was collected between 0930 and 1030h through puncture of the flushing device of shunt systm or the lateral ventricle. The lowest value of MLT detected by HPLC was 15 pg/ml.Melatonin values were higher in patients aged under 10 years than over 20 years in the absence of meningitis or tumor in the pineal region. Even at ages over 15 years, higher CSF MLT values were obtained in the patient … More s with meningitis or tumors in the pineal region. These results suggest that the inflammation or invasion of tumor into the pineal gland may stimulate the secretion of MLT by the pineal gland. However, lower MLT values were obtained in all patients over 40 years old. For these reasons, if one may use the changes of MLT values in CSF as a tumor maker or for determination of the treatment modality, time of CSF collection, age of meningitis should be given due consideration.Also, the presence or absence of the rhythmical changes of melatonin values in a day following circadian rhythm are very important in determination of the treatment modality.Melatonin values in cerebrospinal fluid (CSF) or serum of the pineal cell tumor (PCTS) patients do not always indicate the presence of PCT, and sometimes they go up in presence of the pineal stimulation by the gliomas or GCTs in and around the pineal gland. On the other hand, the histological diagnosis of GCTs and PCTs is sometimes very difficult on hematoxylin and eosin (HE) stained tissue. There are many tumors markers for GCTS, however, the tumor marker of PCTs has not yet been clarified. We studied whether melatonin is a specific tumor marker of PCTs or not using indirect immunocytochemical technique. PCT specimens stained positive with pineoblastoma cell showing higher positivity than pineocytoma cell. However, lower positivity was seen in older tissues of pineoblastoma. No positive stain was seen in all of the GCTs and control specimens. In the specimens of normal pineal glands, the positivity ratio was higher in adult than in the infant.In summary, melatonin is a useful tumor marker of PCTs and immunohistochemical staining for melatonin is a reliable examination for differential diagnosis of PCTs and other tumors. Less

本文应用高效液相色谱法（HPLC）研究了27例脑积水患者脑脊液（CSF）中褪黑素（MLT）的日间变化。患者包括3例先天性脑积水（脊柱裂1例，基亚里II型畸形2例），4例脑膜炎后脑积水，15例脑肿瘤（视交叉生殖细胞瘤3例，额叶恶性胶质瘤3例，颞叶恶性胶质瘤1例;生殖细胞瘤1例，畸胎瘤2例，卵黄囊瘤1例，表皮生殖细胞瘤1例，畸胎瘤2例，卵黄囊瘤1例，松果体区表皮样瘤1例）和正常压力脑积水5例。于09：30 ~ 10：30 h穿刺分流系统冲洗装置或侧脑室收集脑脊液。最低值的MLT检测到的HPLC为15 pg/ml。褪黑激素值高于年龄在10岁以下的患者比20岁以上的脑膜炎或肿瘤的情况下，在松果体区。即使在年龄超过15岁时，患者的CSF MLT值也较高 ...更多信息 患有脑膜炎或松果体区肿瘤的患者。提示炎症或肿瘤侵入松果体可刺激松果体分泌MLT。然而，在所有40岁以上的患者中获得了较低的MLT值。由于这些原因，如果可以使用CSF中MLT值的变化作为肿瘤标志物或用于确定治疗方式，则应适当考虑CSF收集时间、脑膜炎年龄。在一天中褪黑素值是否遵循昼夜节律的节律性变化对于确定治疗方式非常重要。脑脊液（CSF）中褪黑素值或松果体细胞瘤（PCTS）患者血清中PCT的水平并不总是指示PCT的存在，有时它们在松果体中或周围的胶质瘤或GCT的松果体刺激的存在下升高。另一方面，在苏木精和伊红（HE）染色的组织上，GCT和PCT的组织学诊断有时非常困难。GCTS的肿瘤标志物有很多，但PCTs的肿瘤标志物尚未明确。我们用间接免疫细胞化学技术研究褪黑激素是否是PCTs的特异性肿瘤标志物。松果体母细胞瘤PCT染色阳性率高于松果体细胞瘤。然而，在较老的松果体母细胞瘤组织中，阳性率较低。在所有GCT和对照标本中均未观察到阳性染色。在正常松果体标本中，褪黑素的阳性率成人高于婴儿，提示褪黑素是PCTs的一种有用的肿瘤标志物，免疫组化染色是PCTs与其他肿瘤鉴别诊断的可靠方法。少

项目成果

Masahiro Kurisaka: "Immunohistochemical Diagnosis of Pineal Tumors by Monoclonal Antibody for Human Melatonin" Cancer.

Masahiro Kurisaka：“通过人褪黑素单克隆抗体对松果体肿瘤进行免疫组织化学诊断”癌症。

栗坂 昌宏: "各種水頭症例における髄液中メラトニン値の検討" 脳と神経. 43. 631-636 (1991)

Masahiro Kurisaka：“各种脑积水病例中脑脊液褪黑激素水平的检查”《大脑与神经》43. 631-636 (1991)。

栗坂 昌広: "各種水頭症例における髄液中メトラニン値の検討" 脳と神経.

Masahiro Kurisaka：“不同脑积水病例中脑脊液中 metranin 水平的检查”《大脑与神经病学》。

栗坂 昌宏: "メラトニン" ホルモン最近の話題. 12. 14-19 (1991)

Masahiro Kurisaka：“褪黑激素”激素近期主题。12. 14-19 (1991)

栗坂 昌広: "メラトニン" ホルモン最近の話題. 12. 14-19 (1991)

Masahiro Kurisaka：“褪黑激素”激素近期主题。12. 14-19 (1991)