The study Epstein-Barr virus on the disease progression in patients with idiopathic interstitial pneumonia.

EB病毒(EBV)与特发性肺纤维化(LPF)的相关性在最近的文献中存在争议。本研究旨在阐明EB病毒的存在及其对IPF临床特征的影响。20例IPF患者、5例系统性硬化症肺纤维化(SSC)患者和15例正常对照(NC)的肺组织标本均为EBV感染。用两步聚合酶链式反应和免疫组化方法检测EB病毒DNA和EB病毒潜伏膜蛋白-1(LMP1)。分别进行了分析。两步聚合酶链式反应检测IPF患者肺组织的阳性率明显高于NC患者(IPF患者96%，NC_4患者71%，P<0.05)。免疫组织化学显示29例肺前部IPF患者中9例立方上皮细胞呈AND-LMP1和小体阳性。LMP I阳性的IPF患者病情进展的发生率明显高于LN‘IP1阴性患者(P<0.01)。我们的结论是，至少在部分IPF患者中，EBV通过表达LMP1参与了肺纤维化的进展。

The relevance of Epstein-Barr virus (EBV) for idiopathic pulmonary fibrosis (LPF) is controversial in recent literatures. Our aims in this study are to elucidate the existence of the virus as well as to clarify the influences of EBV to the clinical features of IPF.Twenty-time lung specimens from IPF patients and 5 from systemic sclerosis patients with puImonary fibrosis (SSc) and 15 from normal controls (NC) were prepared. Detection of EBV DNA and EBV latent membrane protein-1 (LMP1) were analyzed using two-step PCR methods and immunohistry. respectively. The detection ratio was significantly higher in the lungs with IPF patients than in those with NC by two-step PCR methods (96% in IPF vs 71% in NC_4 P<0.05). ImmunohistochemieaI analysis reveded taht cuboidai epithelial cells were positively stained with and-LMP1 andbody in 9 of 29 lungs front IPF patients. In contrast, none of the SSc patients and NC showed positive staining, It was significant that LMP I -positive IPF patients had more freqnency in "disease progression", defined as if PaO2 decreased snore than 15 torr during foIIow up periods, titan LMP1 -negative paIienis : 5 of 7 LMPI-positive vs 1 of 13 LN'IP1-negative patients ; p<0.01. We concluded that EBV was involved in the progression of the lung fibosis by expressing LMP1, at least, in some of the parients with IPF.